| Project/Area Number |
23658012
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Breeding science
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
TOKI Seiichi 独立行政法人農業生物資源研究所, ゲノム機能改変研究ユニット, ユニット長 (80212067)
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| Co-Investigator(Kenkyū-buntansha) |
SAIKA Hiroaki 農業生物資源研究所, その他部局等, 研究員 (20435613)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 育種学 / 遺伝子 / ゲノム / 植物 / バイオテクノロジー |
|---|
| Research Abstract |
For the establishment of a high efficient gene targeting system via homologous recombination (HR) in higher plants, chemicals that can increase HR frequency were screened. Among 237 kinds of chemicals, 15 chemicals that can increase HR frequency were successfully selected using model system to estimate HR frequency in Arabidopsis plants. The expression analysis of Rad51 gene, which is reported to be induced by DNA double strand breaks (DSBs) showed that 3 chemicals can enhance HR frequency without further inducing DSBs.
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