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Development of core techniques for microbial endosymbiosis engineering

Research Project

Project/Area Number 23658058
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant nutrition/Soil science
Research InstitutionIbaraki University

Principal Investigator

OHTA Hiroyuki  茨城大学, 農学部, 教授 (80168947)

Co-Investigator(Kenkyū-buntansha) NARISAWA Kazuhiko  茨城大学, 農学部, 教授 (90431650)
SATO Yoshinori  独立行政法人国立文化財機構東京文化財研究所, 保存修復科学センター, 研究員 (50466645)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords土壌糸状菌 / 内生細菌 / Morea eongta / Burkhor rtielllaldeia属細菌 / 内生化技術 / ゲノム解析 / 細胞内共生 / 接合菌類 / 共生工学 / エンドファイト / Mortierella / Burkholderia / Pythium
Research Abstract

This study aimed to develop a new technique for the internalization of a useful bacterium into fungal cells, contributing to microbial endosymbiosis engineering. To provide a bacterium-recipient fungus, a technique of making an endobacterium (EB)-free fungus from an EB-possessing fungus was invented. Further, an EB fraction was rtiellasuccessfully recovered from an EB-possessing soil fungus, Morea elongta and its genome sequence was determined. Biosynthetic pathway prediction revealed the lack of sulfur metabolism pathways, suggesting the strict dependence of this endobacterium on the fungus.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (10 results)

All 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (8 results)

  • [Journal Article] 糸状菌細胞に内生する細菌の存在とその検出法2011

    • Author(s)
      佐藤嘉則、成澤才彦、西澤智康、小松崎将一、太田寛行
    • Journal Title

      土と微生物

      Volume: 65巻 Pages: 49-54

    • NAID

      110009468808

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 糸状菌細胞に内生する細菌の存在とその検出法2011

    • Author(s)
      佐藤嘉則・成澤才彦・西澤智康・小松崎将一・太田寛行
    • Journal Title

      土と微生物

      Volume: 65 Pages: 49-54

    • NAID

      110009468808

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 糸状菌Morirea eonaの菌糸内部に生息telllgatする細菌の除去及び再導入化に関する研究2012

    • Author(s)
      大島翔子、佐藤嘉則、西村 歩、藤村玲子、木川りか、成澤才彦、太田寛行
    • Organizer
      第28回日本微生物生態学会大会
    • Place of Presentation
      豊橋
    • Year and Date
      2012-09-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 糸状菌Morirea eona菌糸内telllgat部に生息する細菌のゲノム解析2012

    • Author(s)
      藤村玲子、西村 歩、佐藤嘉則、大島翔子、大島健志朗、服部正平、成澤才彦、太田寛行
    • Organizer
      第28回日本微生物生態学会大会
    • Place of Presentation
      豊橋
    • Year and Date
      2012-09-20
    • Related Report
      2012 Final Research Report
  • [Presentation] Pthpium及びP. nunn に内生する細菌様構造物の検出2011

    • Author(s)
      高島勇介、佐藤嘉則、東條元昭、成澤才彦、y ium ahandermat
    • Organizer
      第27回日本微生物生態学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-10-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Change in the community of associated bacteria with Vernapsis simlxin theoeoperesponse of high temperature stress2011

    • Author(s)
      Rida Khastini 、Yoshinori Sato 、Hiroyuki Ohta、Kazuhiko Narisawa
    • Organizer
      第27回日本微生物生態学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-10-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Change in the community of associated bacteria with Veronaeopsis simplex in2011

    • Author(s)
      Rida Khastini, Yoshinori Sato, Hiroyuki Ohta, Kazuhiko Narisawa
    • Organizer
      日本微生物生態学会第27回大会
    • Place of Presentation
      京都大学
    • Related Report
      2011 Research-status Report
  • [Presentation] Pythium aphanidermatum 及び P. nunn に内生する細菌様構造物の検出2011

    • Author(s)
      高島勇介・佐藤嘉則・東條元昭・成澤才彦
    • Organizer
      日本微生物生態学会第27回大会
    • Place of Presentation
      京都大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 糸状菌Mortierella elongata 菌糸内部に生息する細菌のゲノム解析

    • Author(s)
      藤村玲子,西村歩,佐藤嘉則,大島翔子,大島健志朗,服部正平,成澤才彦,太田寛行
    • Organizer
      第28回日本微生物生態学会大会
    • Place of Presentation
      豊橋技術科学大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 糸状菌Mortierella elongata の菌糸内部に生息する細菌の除去及び再導入化に関する研究

    • Author(s)
      大島翔子,佐藤嘉則,西村歩,藤村玲子,木川りか,成澤才彦,太田寛行
    • Organizer
      第28回日本微生物生態学会大会
    • Place of Presentation
      豊橋技術科学大学
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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