| Project/Area Number |
23658064
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Hokkaido University |
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Principal Investigator |
YOKOTA Atsushi 北海道大学, 大学院・農学研究院, 教授 (50220554)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIZUKA Satoshi 北海道大学, 大学院・農学研究院, 准教授 (00271627)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 二次胆汁酸 / デオキシコール酸 / リトコール酸 / フマル酸 / 嫌気呼吸 / 腸内細菌 / 大腸癌 / 腸溶性カプセル |
|---|
| Research Abstract |
Some secondary bile acids formed in the large intestine are known as a riskfactor of colon cancer. In this study a novel method that reduces such secondary bile acids formed by thereductive metabolism of gut microbes were investigated. We tested if the enhancement of oxidativemetabolism of the responsible gut microbes by the introduction of anaerobic respiration can reduce thetarget secondary bile acids. Thus, rats were fed sodium fumarate-supplemented diet to enhanceanaerobic respiration of the gut microbes. Although the results suggested a possibility of decreasingrelative abundance of deoxycholic acid, a representative colon-cancer-inducing secondary bile acid, theobserved effects were not practically applicable, because high dose of fumarate was required (5~10% ofthe diet), which resulted in the diarrhea in rats experiments. Therefore, improved method of fumaratefeeding, e.g. encapsulation, might be necessary to achieve this goal.
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