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Development of Inhibitors of Biofilm Formation Using Inhibition of Polyamines

Research Project

Project/Area Number 23658079
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionKyoto Institute of Technology

Principal Investigator

Suzuki Hideyuki  京都工芸繊維大学, 工芸科学研究科, 教授 (10202136)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywordsポリアミン / プトレッシン / スペルミジン / 大腸菌 / バイオフィルム / トランスポーター / カダベリン
Outline of Final Research Achievements

We have shown that spermidine is essential for E. coli biofilm formation. In a strain lacking spermidine acetyltransferase, when spermidine was added to the medium, spermidine was not metabolized to acetylspermidine, so that the concentration of spermidine in the cells increased, and the amount of biofilm formed increased accordingly. It was revealed that a strain that could not synthesize spermidine and lacked the spermidine importer PotABCD took up spermidine and became PotFGHI, which was considered to be a spermidine transporter. Furthermore, it was clarified that the presence of PotD protein as well as spermidine is essential for biofilm formation.

Academic Significance and Societal Importance of the Research Achievements

尿路感染症やカテーテルを介した感染において病原菌がバイオフィルムを形成すると難治性に陥りやすく、バイオフィルム形成を阻害する薬剤は重要である。本研究成果は、バイオフィルム形成を阻害する薬剤開発のターゲットとしてPotDタンパク質が重要であることを明らかにした。

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (19 results)

All 2014 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (11 results) (of which Invited: 1 results) Book (4 results)

  • [Journal Article] Properties of putrescine uptake by PotFGHI and PuuP and their physiological significance in Escherichia coli.2014

    • Author(s)
      Terui, Y. et al.
    • Journal Title

      Amino Acids

      Volume: 46 Issue: 3 Pages: 661-670

    • DOI

      10.1007/s00726-013-1517-x

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Upregulation of colonic luminal polyamines produced by intestinal microbiota delays senescence in mice.2014

    • Author(s)
      Kibe R, Kurihara S, Sakai Y, Suzuki H, Ooga T, Sawaki E, Muramatsu K, Nakamura A, Yamashita A, Kitada Y, Kakeyama M. Benno Y. Matsumoto M.
    • Journal Title

      Sci Rep.

      Volume: 4 Issue: 1 Pages: 4548-4548

    • DOI

      10.1038/srep04548

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Putrescine importer PlaP contributes to swarming motility and urothelial cell invasion in Proteus mirabilis.2013

    • Author(s)
      S. Kurihara, Y. Sakai, H. Suzuki, A. Muth, O. Phanstiel, and P. N. Rather.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 288 Issue: 22 Pages: 15668-15676

    • DOI

      10.1074/jbc.m113.454090

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulation mechanism of the putrescine utilization pathway by the transcription factor PuuR in Escherichia coli K-122012

    • Author(s)
      N. Nemoto, S. Kurihara, Y. Kitahara, K. Asada, K. Kato, and H. Suzuki
    • Journal Title

      Journal of Bacteriology

      Volume: (in press) Issue: 13 Pages: 3437-3447

    • DOI

      10.1128/jb.00097-12

    • Related Report
      2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Transcription regulation of puu genes by a transcriptional regulator PuuR in Escherichia coli.2012

    • Author(s)
      H. Suzuki, N. Nemoto, S. Kurihara, Y. Kitahara, K. Asada, and K. Kato.
    • Organizer
      International Congress on “Polyamines Biological and Clinical Perspectives”
    • Place of Presentation
      Istanbul Kultur University Istanbul, Turkey.
    • Related Report
      2012 Research-status Report
  • [Presentation] Spermidine is required for biofilm formation in Escherichia coli.2012

    • Author(s)
      S. Kakegawa, and H. Suzuki.
    • Organizer
      International Congress on “Polyamines Biological and Clinical Perspectives”
    • Place of Presentation
      Istanbul Kultur University Istanbul, Turkey.
    • Related Report
      2012 Research-status Report
  • [Presentation] 大腸菌におけるバイオフィルム形成とポリアミンの関係.2011

    • Author(s)
      掛川苑美、鈴木秀之
    • Organizer
      第3回日本ポリアミン学会年会
    • Place of Presentation
      さいたま市民会館おおみや
    • Related Report
      2011 Research-status Report
  • [Presentation] 腸内細菌がつくりだすシンビオジェニック因子ポリアミン~その機能と生産最適化~.

    • Author(s)
      栗原新、松本光晴、鈴木秀之、辨野義己.
    • Organizer
      2014年度日本農芸化学会大会
    • Place of Presentation
      川崎市・明治大学生田キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 大腸菌におけるバイオフィルム形成とポリアミンの関係.

    • Author(s)
      掛川苑美、鈴木秀之.
    • Organizer
      2014年度日本農芸化学会大会
    • Place of Presentation
      川崎市・明治大学生田キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 大腸菌におけるプトレッシン生産法の開発.

    • Author(s)
      古道紗斗里、鈴木秀之.
    • Organizer
      2014年度日本農芸化学会大会
    • Place of Presentation
      川崎市・明治大学生田キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌の細胞外シグナル分子としてのポリアミンとその輸送.

    • Author(s)
      栗原新、鈴木秀之、Otto Phanstiel IV、Philip N. Rather.
    • Organizer
      日本ポリアミン学会年会
    • Place of Presentation
      銚子市・千葉科学大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 大腸菌におけるプトレッシン生産能の向上.

    • Author(s)
      古道紗斗里、鈴木秀之.
    • Organizer
      日本ポリアミン学会年会
    • Place of Presentation
      銚子市・千葉科学大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 大腸菌のバイオフィルム形成に及ぼすプトレッシンインポーターPlaPとスペルミジンの影響.

    • Author(s)
      掛川苑美、鈴木秀之.
    • Organizer
      日本ポリアミン学会年会
    • Place of Presentation
      銚子市・千葉科学大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 大腸菌におけるバイオフィルム形成とポリアミンの関係.

    • Author(s)
      掛川苑美、鈴木秀之.
    • Organizer
      「ポリアミンと核酸の共進化」第12回合同シンポジウム
    • Place of Presentation
      東京都・東京慈恵会医科大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌のポリアミントランスポーターとその生理的役割.

    • Author(s)
      鈴木秀之、掛川苑美、栗原新
    • Organizer
      平成25年度日本農芸化学会大会 シンポジウム
    • Place of Presentation
      東北大学、仙台
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] Bacterial polyamine transporters. In Polyamines: A universal molecular nexus for growth, survival and specialized metabolism.2014

    • Author(s)
      S. Kurihara, and H. Suzuki.
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report
  • [Book] Polyamine catabolism in prokaryotes. In Polyamines: A universal molecular nexus for growth, survival and specialized metabolism.2014

    • Author(s)
      H. Suzuki, and S. Kurihara.
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report
  • [Book] Microbial production of food ingredients, enzymes and nutraceuticals.2013

    • Author(s)
      H. Suzuki
    • Publisher
      Woodhead Publishing
    • Related Report
      2012 Research-status Report
  • [Book] Handbook of Proteolytic Enzymes. 3rd ed. Vol. 12013

    • Author(s)
      J. Hiratake, H. Suzuki, K. Fukuyama, K. Wada, and H. Kumagai.
    • Publisher
      Academic Press
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2022-01-27  

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