Why don't you try negative ionization? Alternative challenge to solve inherent problems in proteomics
| Project/Area Number |
23659016
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
OE Tomoyuki 東北大学, 大学院・薬学研究科, 教授 (10203712)
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| Co-Investigator(Kenkyū-buntansha) |
GOTO Takaaki 東北大学, 大学院・薬学研究科, 講師 (40344684)
LEE Seonhwa 東北大学, 大学院・薬学研究科, 助教 (60519776)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 分析化学 / プロテオミクス / 質量分析 / 負イオン / 化学修飾 |
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| Research Abstract |
Optimization and application of negative ionization MALDI
●MALDI conditions have been optimized for negative ionization.
●Novel peptide mass fingerprinting method has been developed using negative ionization.
●Novel screening method for chemical modifications on HSA has been developed.
Optimization and application of negative ionization ESI
● ESI conditions have been optimized for negative ionization
●Fragmentation pattern has been investigated for each chemically modified peptide.
●A screening method for chemical modifications has been developed using the specific fragmentations.
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Report
(3 results)
Research Products
(27 results)
