Development of a yeast-based high-throughput screening system for therapeutic drugs against amyloidoses
| Project/Area Number |
23659045
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Nagoya City University |
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Principal Investigator |
HOSHINO Shinichi 名古屋市立大学, 大学院・薬学研究科, 教授 (40219168)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 分子生物学 / 翻訳終結 / eRF3 / 酵母プリオン / 翻訳終結因子 / アミロイドーシス / 終止コドン |
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| Research Abstract |
In the present study, we have constructed a yeast strain expressing chimeric prion protein in which prion domain of the yeast eRF3 (Sup35) was replaced by that of human PrP. Most of the modified yeast cells spontaneously transformed to [PSI^+] and the [PSI^+] transformants were returned to [psi^-] when the cells were treated with guanidine hydrochrolide. The eRF3-PrP prion had no effect on the growth of yeast cells. Thus, we confirmed that the modified [PSI^+] prion, the amyloidgenic isoform of eRF3-PrP, could be an ideal model for investigating prion disease in yeast and for screening therapeutic drugs against prion.
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Report
(3 results)
Research Products
(39 results)
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[Journal Article] Biological role of the two overlapping poly(A)-binding protein interacting motifs 2 (PAM2) of eukaryotic releasing factor eRF3 in mRNA decay2012
Author(s)
Osawa, M., Hosoda, N., Nakanishi, T., Uchida, N., Kimura, T., Imai, S., Machiyama, A., Katada, T., Hoshino, S., Shimada, I
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Journal Title
RNA
Volume: 18
Pages: 1957-1967
Related Report
Peer Reviewed
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[Journal Article] Anti-proliferative protein Tob negatively regulates CPEB3 target by recruiting Caf1 deadenylase2011
Author(s)
Hosoda, N., Funakoshi, Y., Hirasawa, M., Yamagishi, R., Asano, Y., Miyagawa, R., Ogami, K., Tsujimoto, M., Hoshino, S.
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Journal Title
EMBO J
Volume: 30
Pages: 1311-1323
Related Report
Peer Reviewed
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