Research Project
Grant-in-Aid for Challenging Exploratory Research
In the present study, we have constructed a yeast strain expressing chimeric prion protein in which prion domain of the yeast eRF3 (Sup35) was replaced by that of human PrP. Most of the modified yeast cells spontaneously transformed to [PSI^+] and the [PSI^+] transformants were returned to [psi^-] when the cells were treated with guanidine hydrochrolide. The eRF3-PrP prion had no effect on the growth of yeast cells. Thus, we confirmed that the modified [PSI^+] prion, the amyloidgenic isoform of eRF3-PrP, could be an ideal model for investigating prion disease in yeast and for screening therapeutic drugs against prion.
All 2013 2012 2011 2010
All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (26 results)
J Biol Chem
Volume: (in press)
CPEB. Oncogene
Biochem Biophys Res Commun
Volume: 432 Pages: 135-140
Biochem Biophys Res Commun.
Volume: 432 Issue: 1 Pages: 135-140
10.1016/j.bbrc.2013.01.072
Oncogene
Volume: - Issue: 1 Pages: 55-64
10.1038/onc.2012.548
Apoptosis
Volume: 17 Pages: 1287-1299
Wiley Interdiscip Rev RNA
Volume: 3 Pages: 743-757
RNA
Volume: 18 Pages: 1957-1967
Volume: 17 Issue: 12 Pages: 1287-1299
10.1007/s10495-012-0765-7
Volume: 3 Issue: 6 Pages: 743-757
10.1002/wrna.1133
Volume: 18 Issue: 11 Pages: 1957-1967
10.1261/rna.035311.112
EMBO J
Volume: 30 Pages: 1311-1323
EMBO J.
Volume: 30 Pages: 1311-23
