Development of effective blood purification treatment method usingHSA bound toxic comnpodus designed bpyhase display tecnohlogy

• Project/Area Number: 23659088
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Sojo University
• Principal Investigator: OTAGIRI Masaki 崇城大学, 薬学部, 教授 (80120145)
• Co-Investigator(Kenkyū-buntansha): MARUYAMA Toru 熊本大学, 薬学部, 教授 (90423657) ; WATANABE Hiroshi 熊本大学, 薬学部, 准教授 (70398220)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: アルブミン / 血液浄化法 / 分子進化工学 / 毒素中和 / ハイブリッド / 毒性中和

Research Abstract

This study was undertaken to design and evaluate recombinant albumin mutant( specially, domain II mutant)with high bilirubin (BR) binding affinity via phage display technology.The results obtained here identified the amino acid residues that interact carboxylatesof BR in HSA, and also produces HSA domain II mutants with high BR binding affinity. To our knowledge, this is the first finding on HSA mutant with enhanced binding affinity to BR. This approach could be applied to other HSA bound toxic compounds that responsible for the progression oifseadse and thereby it will pave the way for the development ofminimally invasive and low cost blood clarification method.

Research Products

• [Journal Article] Long-acting human serum albumin-thioredoxin fusion protein suppresses bleomycin-induced pulmonary fibrosis progression. (2013)
  • Author(s): Tanaka R, Watanabe H, Kodama A, Chuang VT, Ishima Y, Hamasaki K, Tanaka K, Mizushima T, Otagiri M, Maruyama T.
  • Journal Title: J Pharmacol Exp Ther. Volume: 345 Issue: 2 Pages: 271-283
  • DOI: 10.1124/jpet.112.201814
  • Peer Reviewed
• [Journal Article] UW solution improved with high anti-apoptotic activity by S-nitrosated human serum albumin. (2013)
  • Author(s): Ishima Y
  • Journal Title: Nitric Oxide. Volume: 印刷中 Pages: 36-42
  • DOI: 10.1016/j.niox.2013.01.004
  • Peer Reviewed
• [Journal Article] Albumin domain II mutant with high bilirubin binding affinity has a great potential as serum bilirubin excretion enhancer for hyperbilirubinemia treatment. (2013)
  • Author(s): Minomo A
  • Journal Title: Biochim Biophys Acta. Volume: 印刷中 Issue: 4 Pages: 141-8
  • DOI: 10.1016/j.bbagen.2013.01.006
  • Peer Reviewed
• [Journal Article] Pharmacokinetic alteration of (99m)Tc-MAG3 using serum protein binding displacement method. (2013)
  • Author(s): Nishi K, Kobayashi M, Nishii R, Shikano N, Takamu ra N, Kuga N, Yamasaki K, Nagamachi S, Tamura S, Otagiri M, Kawai K.
  • Journal Title: Nucl Med Biol. Volume: 40 Pages: 366-70
  • Peer Reviewed
• [Journal Article] A human serum albumin-thioredoxin fusion protein prevents experimental contrast-induced nephropathy. (2013)
  • Author(s): Kodama A
  • Journal Title: Kidney Int. Volume: 印刷中 Issue: 3 Pages: 446-54
  • DOI: 10.1038/ki.2012.429
  • Peer Reviewed
• [Journal Article] Carbon Monoxide Bound Red Blood Cells Protect Red Blood Cell Transfusion-induced Hepatic Cytochrome P450 Impairment in Hemorrhagic-shock Rats. Drug Metab Dispos. (2013)
  • Author(s): Ogaki S, Taguchi K, Watanabe H, Otagiri M, and Maruyama T.
  • Journal Title: Drug Metab Dispos. Volume: 41 Issue: 1 Pages: 141-8
  • DOI: 10.1124/dmd.112.048744
  • Peer Reviewed
• [Journal Article] S-nitrosylated a-l-acid glycoprotein kills drug-resistant bacteria and aids survival in sepsis (2013)
  • Author(s): Watanabe K, Ishima Y, Akaike T, Sawa T, Kuroda T, Ogawa W, Watanabe H, Suenaga A, Kai T, Otagiri M, and Maruyama T.
  • Journal Title: FASEB J Volume: 27 Issue: 1 Pages: 391-398
  • DOI: 10.1096/fj.12-217794
  • Peer Reviewed
• [Journal Article] Interaction between two sulfate-conjugated uremic toxins, p-cresyl sulfate and indoxyl sulfate, during binding with human serum albumin. (2013)
  • Author(s): Watanabe H
  • Journal Title: Biochim Biophys Acta. Volume: 40 Issue: 7 Pages: 1423-8
  • DOI: 10.1124/dmd.112.045617
  • Peer Reviewed
• [Journal Article] Effect of acetaminophen on progression of renal damage in adenine induced renal failure model rat. (2012)
  • Author(s): Kadowaki D, Sumikawa S, Arimizu K, Taguchi K, Kitamura K, Ishitsuka Y, Narita Y, Irie T, Maruyama T, Otagiri M, Hirata S
  • Journal Title: Life Sci. Volume: 91 Issue: 25-26 Pages: 1304-1308
  • DOI: 10.1016/j.lfs.2012.09.018
  • Peer Reviewed
• [Journal Article] Effect of acetaminophen on the progression of renal damage in adenine induced renal failure model rats. (2012)
  • Author(s): Kadowaki D, Sumikawa S, Arimizu K, Taguchi K, Kitamura K, Ishitsuka Y, Narita Y, Irie T, Chuang VT, Maruyama T, Otagiri M, Hirata S.
  • Journal Title: Life Sci Volume: 91 Pages: 1304-8
  • Peer Reviewed
• [Journal Article] Elucidation of the therapeutic enhancer mechanism of poly -S-nitrosated human serum albumin against multidrug-resistant tumor in animal models. (2012)
  • Author(s): Ishima Y, Hara M, Kragh-Hansen U, Inoue A, Suenaga A, Kai T, Watanabe HOtagiri M, Maruyama T .
  • Journal Title: J Control Release. Volume: 164 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Liposome-encapsulated hemoglobin (hemoglobin-vesicle) is not transferred from mother to fetus at the late stage of pregnancy in the rat model. (2012)
  • Author(s): Kaga M, Li H, Ohta H, Taguchi K, Ogaki S, Izumi H, Inagaki M, Tsuchiya S, Okamura K, Otagiri M, Sakai H, and Yaegashi N.
  • Journal Title: Life Sci. Volume: 91 Issue: 11-12 Pages: 420-8
  • DOI: 10.1016/j.lfs.2012.08.021
  • Peer Reviewed
• [Journal Article] A uremic toxin, 3-carboxy-4-methyl-5-propyl-2-furanpropionate induces cell damage to proximal tubular cells via the generation of a radical intermediate. (2012)
  • Author(s): Miyamoto Y, Iwao Y, Mera K, Watanabe H, Kadowaki D, Ishima Y, Chuang VT, Sato K, Otagiri M, Maruyama T.
  • Journal Title: Biochem Pharmacol. Volume: 84 Pages: 1207-14
  • Peer Reviewed
• [Journal Article] α(1)-Acid glycoprotein up -regulates CD163 via TLR4/CD14 protein pathway: possible protection against hemolysis-induced oxidative stress. (2012)
  • Author(s): Komori H, Watanabe H, Shuto T, Kodama A,Maeda H, Watanabe K, Kai H, Otagiri M, Maruyama T.
  • Journal Title: J Biol Chem.
  • Peer Reviewed
• [Journal Article] Pharmaceutical aspects of the recombinant human serum albumin dimer: structural characteristics, biological properties, and medical applications. (2012)
  • Author(s): Taguchi K, Chuang VT, Maruyama T, Otagiri M.
  • Journal Title: J Pharm Sci. Volume: 101 Issue: 9 Pages: 3033-46
  • DOI: 10.1002/jps.23181
  • Peer Reviewed
• [Journal Article] S-Guanylation of human serum albumin is a unique posttranslational modification and results in a novel class of antibacterial agents (2012)
  • Author(s): Ishima Y, Hoshino H, Shinagawa T, Watanabe K, Akaike T, Sawa T, Kragh-Hansen U, Kai T, Watanabe H, Maruyama T, and Otagiri M
  • Journal Title: J. Pharm. Sci Volume: 101 Issue: 9 Pages: 3222-3229
  • DOI: 10.1002/jps.23143
  • Peer Reviewed
• [Journal Article] Quantitative evaluation of the role of cysteine and methionine residues in the antioxidant activity of human serum albumin using recombinant mutants (2012)
  • Author(s): Iwao Y
  • Journal Title: IUBMB Life Volume: 64 Issue: 5 Pages: 450-454
  • DOI: 10.1002/iub.567
  • Peer Reviewed
• [Journal Article] Arginine 485 of human serum albumin interacts with the benzophenone moiety of ketoprofen in the binding pocket of subdomain III A and III B. (2012)
  • Author(s): Kaneko K, Chuang VT, Ito T, Suenaga A, Watanabe H, Maruyama T, Otagiri M. Pharmazie.
  • Journal Title: Pharmazie. Volume: 67 Pages: 414-8
  • Peer Reviewed
• [Journal Article] α1-acid glycoprotein up-regulates CD163 via TLR4/CD14 pathway: possible protection against hemolysis-induced oxidative stress. (2012)
  • Author(s): Komori H, Watanabe H, Shuto T, Kodama A, Maeda H, Watanabe K, Kai H, Otagiri M, Maruyama T.
  • Journal Title: J Biol Chem. Volume: 287 Issue: 36 Pages: 30688-700
  • DOI: 10.1074/jbc.m112.353771
  • Peer Reviewed
• [Journal Article] Pharmaceutical aspects of the recombinant human serum albumin dimer: Structural characteristics, biological properties, and medical applications. (2012)
  • Author(s): Taguchi K, Giam Chuang VT, Maruyama T, Otagiri M.
  • Journal Title: J Pharm Sci. Volume: 101 Pages: 3033-46
  • Peer Reviewed
• [Journal Article] A fourteen-day observation and pharmacokinetic evaluation after a massive intravenous infusion of hemoglobin-vesicles (artificial oxygen carriers) in cynomolgus monkeys. (2012)
  • Author(s): Taguchi K, Watanabe H, Sakai H, Horinouchi H, Kobayashi K, Maruyama T, and Otagiri M
  • Journal Title: J Drug Metab Toxicol. Volume: 3 Issue: 4 Pages: 128-34
  • DOI: 10.4172/2157-7609.1000128
  • Peer Reviewed
• [Journal Article] Elucidation of the therapeutic enhancer mechanism of poly-S-nitrosated human serum albumin against multidrug-resistant tumor in animal models. (2012)
  • Author(s): Ishima Y
  • Journal Title: J Control Release. Volume: 164 Issue: 1 Pages: 1-7
  • DOI: 10.1016/j.jconrel.2012.10.003
  • Peer Reviewed
• [Journal Article] Characterization of the hepatic cellular uptake of α(1) -acid glycoprotein (AGP), part 1: A peptide moiety of human AGP is recognized by the hemoglobin β-chain on mouse liver parenchymal cells. (2012)
  • Author(s): Nishi K, Komori H, Kikuchi M, Uehara N, Fukunaga N, Matsumoto K, Watanabe H, Nakajou K, Misumi S, Suenaga A, Maruyama T, Otagiri M.
  • Journal Title: J Pharm Sci. Volume: 101 Pages: 1599-606
  • Peer Reviewed
• [Journal Article] Characterization of hepatic cellular uptake of α1-acid glycoprotein (AGP), part 2: Involvement of hemoglobin β-chain on plasma membranes in the uptake of human AGP by liver parenchymal cells. (2012)
  • Author(s): Komori H, Nishi K, Uehara N, Watanabe H, Shuto T, Suenaga A, Maruyama T, Otagiri M.
  • Journal Title: J Pharm Sci. Volume: 101 Pages: 1607-15
  • Peer Reviewed
• [Journal Article] Quantitative evaluation of the role of cysteine and methionine residues in the antioxidant activity of human serum albumin using recombinant mutants. (2012)
  • Author(s): Iwao Y, Ishima Y, Yamada J, Noguchi T, Kragh-Hansen U, Mera K, Honda D, Suenaga A, Maruyama T, Otagiri M.
  • Journal Title: IUBMB Life Volume: 64 Pages: 450-54
  • Peer Reviewed
• [Journal Article] Characterization of the hepatic cellular uptake of α_1-acid glycoprotein (AGP), Part 2 : Involvement of the hemoglobin β-chain on plasma membranes in the uptake of human AGP by liver parenchymal cells (2011)
  • Author(s): 小森久和
  • Journal Title: Journal of Pharmaceutical Sciences Volume: 101 Issue: 4 Pages: 1607-1615
  • DOI: 10.1002/jps.22804
  • Peer Reviewed
• [Journal Article] Characterization of the hepatic cellular uptake of α_1-acid glycoprotein (AGP), Part 1 : A peptide moiety of human AGP is recognized by the hemoglobin β-chain on mouse liver parenchymal cells (2011)
  • Author(s): 西弘二、小森久和
  • Journal Title: Journal of Pharmaceutical Sciences Volume: 101 Issue: 4 Pages: 1599-1606
  • DOI: 10.1002/jps.23015
  • Peer Reviewed
• [Journal Article] Alteration in the pharmacokinetics of hemoglobin-vesicles in a rat model of chronic liver cirrhosis is associated with Kupffer cell phagocyte activity. (2011)
  • Author(s): Taguchi K, Miyasato M, Watanabe H, Sakai H, Tsuchida E, Horinouchi H, Kobayashi K, Maruyama T, Otagiri M
  • Journal Title: J Pharm Sci. Volume: 100 Pages: 775-83
  • Peer Reviewed
• [Journal Article] Repeated injection of high doses of hemoglobin-encapsulated liposomes (hemoglobin vesicles) induces accelerated blood clearance in a hemorrhagic shock rat model. (2011)
  • Author(s): Taguchi K, Iwao Y, Watanabe H, Kadowaki D, Sakai H, Kobayashi K, Horinouchi H, Maruyama T, Otagiri M.
  • Journal Title: Drug Metab Dispos. Volume: 26 Pages: 2498-502
  • Peer Reviewed
• [Journal Article] Fluid resuscitation with hemoglobin vesicles prevents Escherichia coli growth via complement activation in a hemorrhagic shock rat model. (2011)
  • Author(s): Taguchi K, Ogaki S, Watanabe H, Kadowaki D, Sakai H, Kobayashi K, Horinouchi H, Maruyama T, Otagiri M.
  • Journal Title: J Pharmacol Exp Ther. Volume: 337 Pages: 201-8
  • Peer Reviewed
• [Journal Article] Organic anion transporters play an important role in the uptake of p-cresyl sulfate, a uremic toxin, in the kidney. (2011)
  • Author(s): Miyamoto Y, Watanabe H, Noguchi T, Kotani S, Nakajima M, Kadowaki D, Otagiri M, Maruyama T.
  • Journal Title: Nephrol Dial Transplant. Volume: 26 Pages: 2498-502
  • Peer Reviewed
• [Journal Article] Spectroscopic studies of interactions of chondroitin sulfates with cisplatin. (2011)
  • Author(s): Zhang JS, Anraku M, KadowakiD, Imai T, Suenaga A, Odai A, Otagiri M
  • Journal Title: Carbohydr Res. Volume: 346 Pages: 631-637
  • Peer Reviewed
• [Journal Article] Structural insights into differences in drug-binding selectivity between two forms of human alpha1-acid glycoprotein genetic variants, the A and F1*S forms. (2011)
  • Author(s): Nishi K, Ono T, Nakamura T, Fukunaga N, Izumi M, Watanabe H, Suenaga A, Maruyama T, Yamagata Y, Curry S,Otagiri M
  • Journal Title: J Biol Chem. Volume: 286 Pages: 14427-34
  • Peer Reviewed
• [Journal Article] An autoantibody against N(ε)-(carboxyethyl)lysine (CEL): possible involvement in the removal of CEL-modified proteins by macrophages. (2011)
  • Author(s): Mera K, Nagai R, Takeo K, Izumi M, Maruyama T, Otagiri M.
  • Journal Title: Biochem Biophys Res Commun. Volume: 407 Pages: 420-5
  • Peer Reviewed
• [Journal Article] Vitamin D receptor activator reduces oxidative stress in hemodialysis patients with secondary hyperparathyroidism. (2011)
  • Author(s): Tanaka M, Tokunaga K, Komaba H, Itoh K, Matsushita K, Watanabe H, Kadowaki D, Maruyama T, Otagiri M, Fukagawa M.
  • Journal Title: Ther Apher Dial. Volume: 15 Pages: 161-8
  • Peer Reviewed
• [Journal Article] Quantitative analysis of cysteine-34 on the anitioxidative properties of human serum albumin in hemodialysis patients. (2011)
  • Author(s): Anraku M, Takeuchi K, Watanabe H, Kadowaki D, Kitamura K, Tomita K, Kuniyasu A, Suenaga A, Maruyama T, Otagiri M.
  • Journal Title: J Pharm Sci. Volume: 100 Pages: 3968-76
  • Peer Reviewed
• [Journal Article] Histidine146 of human serum albumin plays a prominent role at the interface of subdomains IA and IIA in allosteric ligand binding. (2011)
  • Author(s): Kaneko K, Chuang VT, Minomo A, Yamasaki K, Bhagavan NV, Maruyama T, Otagiri M
  • Journal Title: IUBMB Life. Volume: 63 Pages: 277-85
  • Peer Reviewed
• [Journal Article] Loss of functional albumin triggers acceleration of transthyretin amyloid fibril formation in familial amyloidotic polyneuropathy. (2011)
  • Author(s): Kugimiya T, Jono H, Saito S, Maruyama T, Kadowaki D, Misumi Y, Hoshii Y, Tasaki M, Su Y, Ueda M, Obayashi K, Shono M, Otagiri M, Ando Y.
  • Journal Title: Lab Invest. Volume: 91 Pages: 1219-28
  • Peer Reviewed
• [Journal Article] Parathyroidectomy markedly reduces oxidative stress in a patient with primary hyperparathyroidism. (2011)
  • Author(s): Tanaka M, Tokunaga K, Maruyama T, Otagiri M, Tominaga Y, Itoh K, Matsushita K, Komaba H, Fukagawa M
  • Journal Title: Ther Apher Dial. Volume: 15 Pages: 38-41
  • Peer Reviewed
• [Journal Article] Human serum albumin-thioredoxin fusion protein with long blood retention property is effective in suppressing lung injury. (2011)
  • Author(s): Furukawa M, Tanaka R, Chuang VT, Ishima Y, Taguchi K, Watanabe H, Maruyama T, Otagiri M.
  • Journal Title: J Control Release. Volume: 154 Pages: 189-95
  • Peer Reviewed
• [Journal Article] Toxicodynamic evaluation of a cisplatin-chondroitin sulfate complex using a perfused kidney and human proximal tubular cells. (2011)
  • Author(s): Zhang JS, Kadowaki D, Nonoguchi H, Hirata S, Seo H, Imai T, Suenaga A, Giam Chuang VT, Otagiri M.
  • Journal Title: Ren Fail. Volume: 33 Pages: 609-14
  • Peer Reviewed
• [Journal Article] Cellular uptake mechanisms and responses to NO transferred from mono- and poly-S-nitrosated human serum albumin. (2011)
  • Author(s): Ishima Y, Yoshida F, Kragh-Hansen U, Watanabe K, Katayama N, Nakajou K, Akaike T, Kai T, Maruyama T, Otagiri M.
  • Journal Title: Free Radic Res. Volume: 45 Pages: 1196-206
  • Peer Reviewed
• [Journal Article] Biological characteristics of two lysines on human serum albumin in the high-affinity binding of 4Z,15Z-bilirubin-IXα revealed by phage display. (2011)
  • Author(s): Minomo A, Ishima Y, Kragh-Hansen U, Chuang VT, Uchida M, Taguchi K, Watanabe H, Maruyama T, Morioka H, Otagiri M.
  • Journal Title: FEBS J. Volume: 278 Pages: 4100-11
  • Peer Reviewed
• [Journal Article] S-Nitrosated Human Serum Albumin Dimer is not only a Novel Anti-Tumor Drug but also a Potentiator for Anti-Tumor Drugs with Augmented EPR Effects.ｆ (2011)
  • Author(s): Ishima Y, Chen D, Fang J, Maeda H, Minomo A, Kragh-Hansen U, Kai T, Maruyama T, Otagiri M.
  • Journal Title: Bioconjug Chem. Volume: 23 Pages: 264-71
  • Peer Reviewed
• [Journal Article] New insight into the redox properties of uremic solute indoxyl sulfate as a pro- and anti-oxidant. (2011)
  • Author(s): Miyamoto Y, Watanabe H, Otagiri M, Maruyama T.
  • Journal Title: Ther Apher Dial. Volume: 15 Pages: 129-31
  • Peer Reviewed
• [Journal Article] Pharmacokinetic properties of hemoglobin vesicles as a substitute for red blood cells. (2011)
  • Author(s): Taguchi K, Maruyama T, Otagiri M.
  • Journal Title: Drug Metab Rev. Volume: 43 Pages: 362-73
  • Peer Reviewed
• [Journal Article] Update on the pharmacokinetics and redox properties of protein-bound uremic toxins. (2011)
  • Author(s): Watanabe H, Miyamoto Y, Otagiri M,Maruyama T.
  • Journal Title: J Pharm Sci. Volume: 100 Pages: 3682-95
  • Peer Reviewed
• [Presentation] ブレオマイシン誘発肺線維症に対するアルブミン-チオレドキシン融合タンパク質の有用性評価 (2013)
  • Author(s): 小田切優樹
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2013-03-28
• [Presentation] S-ニトロソ化により獲得するα1-酸性等タンパク質(AGP)の抗菌活性 (2013)
  • Author(s): 異島優
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: ィコ横浜
  • Year and Date: 2013-03-28
• [Presentation] エリスロポエチン骨髄デリバリーシステムの構築と腎性貧血治療への応用 (2013)
  • Author(s): 丸山徹
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2013-03-28
• [Presentation] エリスロポエチン骨髄デリバリーシステムの構築と腎性貧血治療への応用 (2013)
  • Author(s): 丸山 徹
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜（神奈川県
• [Presentation] S-ニトロソ化により獲得するα1－酸性等タンパク質（AGP）の抗菌活性 (2013)
  • Author(s): 異島優
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜（神奈川県
• [Presentation] ブレオマイシン誘発肺線維症に対するアルブミン-チオレドキシン融合タンパク質の有用性評価 (2013)
  • Author(s): 小田切優樹
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜（神奈川県
• [Presentation] ヒト血清アルブミン-チオレドキシン融合タンパク質の持続的な抗酸化・抗炎症作用によるブレオマイシン誘発肺線維症に対する治療効果 (2012)
  • Author(s): 田中遼大
  • Organizer: 日本薬物動態学会第27回年会
  • Place of Presentation: タワーホール船堀
• [Presentation] 尿毒症物質p-クレジル硫酸はOATsを介して近位尿細管上皮、血管内皮及び血管平滑筋細胞に取り込まれ酸化ストレス障害を誘発する (2012)
  • Author(s): 渡邊博志
  • Organizer: 日本薬物動態学会第27回年会
  • Place of Presentation: タワーホール船堀
• [Presentation] 高分子抗癌剤の腫瘍移行制御特性を有するSNO-Albumin Dimerの設計 (2012)
  • Author(s): 異島優
  • Organizer: 日本薬物動態学会第27回年会
  • Place of Presentation: タワーホール船堀
• [Presentation] 一酸化炭素付加型ヘモグロビン小胞体は抗炎症・抗酸化作用を介してブレオマイシン誘発肺線維症を抑制する (2012)
  • Author(s): 永尾 紗理
  • Organizer: 日本薬物動態学会第27回年会
  • Place of Presentation: タワーホール船堀
• [Presentation] ヒト血清アルブミン-チオレドキシン融合タンパク質は抗酸化作用によりシスプラチン誘発腎障害を抑制する (2012)
  • Author(s): 小玉あずさ
  • Organizer: 日本薬物動態学会第27回年会
  • Place of Presentation: タワーホール船堀
• [Presentation] ヒトa1-酸性糖タンパク質バリアントA体とF1*S体に対する薬物結合選択性の分子機序解明 (2012)
  • Author(s): 丸山徹
  • Organizer: 日本薬物動態学会第27回年会
  • Place of Presentation: タワーホール船堀
• [Presentation] 一酸化炭素付加赤血球によるクッパー細胞の不活化は輸血誘発肝チトクロームP450機能障害を保護する (2012)
  • Author(s): 大柿滋
  • Organizer: 第34回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 京都大学薬学部
• [Presentation] 尿毒症物質p-クレジル硫酸のレドックス特性と腎障害心血管疾患発症機序解明 (2012)
  • Author(s): 宮本洋平
  • Organizer: 第34回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 京都大学薬学部
• [Presentation] S-ニトロソ化に伴うα1-酸性糖タンパク質(AGP)の抗菌機能獲得と感染症治療への応用 (2012)
  • Author(s): 渡辺佳織
  • Organizer: 第34回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 京都大学薬学部
• [Presentation] ラット妊娠母体におけるヘモグロビン小胞体の胎盤通過性 (2012)
  • Author(s): 太田英伸
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール
• [Presentation] 血小板代替物H12(ADP)リポソームの体内動態に及ぼす血小板減少症の影響 (2012)
  • Author(s): 丸山徹
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール
• [Presentation] 細胞型人工酸素運搬体ヘモグロビン小胞体のカニクイザルへの大量投与の結果からみた実効性 (2012)
  • Author(s): 田口和明
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール
• [Presentation] ESI-TOF/MSを用いたシステイン付加型ヒト血清アルブミンの検出と機能相関ー慢性肝疾患の影響ー (2012)
  • Author(s): 南雲恒平
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール
• [Presentation] 出血性ショックも出るラットにおける一酸化炭素付加型赤血球の肝地とクロームP450保護効果 (2012)
  • Author(s): 大柿滋
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール
• [Presentation] The potential of albumin for use as a nanomedicine carrier (2012)
  • Author(s): Otagiri M
  • Organizer: Montr eal2012 international forum on enviroment and medicine.
  • Place of Presentation: Montreal,Quebec Canada
• [Presentation] ヒト血清アルブミンドメインIIによるビリルビン尿中排泄促進作用 (2012)
  • Author(s): 小田切優樹
  • Organizer: 第28回日本DDS学会学術集会
  • Place of Presentation: 札幌コンベンションセンター
• [Presentation] 高分子抗癌剤の腫瘍集積性を高めるS-ニトロソ化アルブミンダイマーのEPR効果増強作用 (2012)
  • Author(s): 異島優
  • Organizer: 第28回日本DDS学会学術集会
  • Place of Presentation: 札幌コンベンションセンター
• [Presentation] S-ニトロソ化アルブミンにより抗アポトーシス効果を付与した改良型臓器保存液の開発 (2012)
  • Author(s): 異島優
  • Organizer: 第12回日本NO学会学術集会
  • Place of Presentation: 神戸国際会議場
• [Presentation] S-ニトロソ化に伴うα1-酸性糖タンパク質(AGP)の抗菌機能獲得と感染症治療への応用 (2012)
  • Author(s): 渡辺 佳織
  • Organizer: 第12回日本NO学会学術集会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Human serum albumin as a nanomedecine carrir (2012)
  • Author(s): Otagiri M
  • Organizer: Nanoformulation 2012
  • Place of Presentation: Fira Palace, Barcelona (Spain)
• [Presentation] p -Cresyl sulfate causes renal tubular cell damage by inducing oxidative stress through the activation of NADPH oxidase (2012)
  • Author(s): Hiroshi Watanabe
  • Organizer: 49th ERA-EDTA Congress, Paris 2012
  • Place of Presentation: Paris(France)
• [Presentation] A uremic toxin, 3-carboxy-4-methyl-5-propyl-2-furanpropionate accumulates in proximal tubular cells and induces cell damage through increasing oxidative stress (2012)
  • Author(s): Yohei Miyamoto
  • Organizer: 49th ERA-EDTA Congress,
  • Place of Presentation: Paris (France)
• [Presentation] 新規翻訳後修飾体であるS -グアニル化アルブミンは抗菌効果を発揮する (2012)
  • Author(s): 異島 優
  • Organizer: 日本薬剤学会第27年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] S-ニトロソ化アルブミンダイマーはEPR効果を増強する (2012)
  • Author(s): 井上亜希
  • Organizer: 日本薬剤学会第27年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] ブレオマイシン誘発肺線維症に対するアルブミン-チオレドキシン融合体の有用性評価 (2012)
  • Author(s): 田中遼大
  • Organizer: 日本薬剤学会第27年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 骨髄指向性を有する新規エリスロポエチン製剤の開発 (2012)
  • Author(s): 宮崎裕理
  • Organizer: 日本薬剤学会第27年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] アルギニンペプチドを利用した細胞膜透過型アルブミンの設計と評価 (2012)
  • Author(s): 濱崎慶輔
  • Organizer: 日本薬剤学会第27年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Invivoファージディスプレイ法を用いた新規腎送達ペプチドの探索 (2012)
  • Author(s): 弥永直樹
  • Organizer: 日本薬剤学会第27年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] S -ニトロソ化アルブミンダイマーはEPR効果を増強する (2012)
  • Author(s): 井上 亜希
  • Organizer: 日本薬剤学会第27年会
  • Place of Presentation: 神戸国際会議場（兵庫県）
• [Presentation] アルギニンペプチドを利用した細胞膜透過型アルブミンの設計と評価 (2012)
  • Author(s): 濱崎 慶輔
  • Organizer: 日本薬剤学会第27年会
  • Place of Presentation: 神戸国際会議場（兵庫県）
• [Presentation] In vivoファージディスプレイ法を用いた新規腎送達ペプチドの探索 (2012)
  • Author(s): 弥永 直樹
  • Organizer: 日本薬剤学会第27年会
  • Place of Presentation: 神戸国際会議場（兵庫県）
• [Presentation] S-ニトロソ化に伴うα1-酸性糖タンパク質（AGP）の抗菌機能獲得と感染症治療への応用 (2012)
  • Author(s): 渡辺 佳織
  • Organizer: 第12回日本NO学会学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県）
• [Presentation] S-ニトロソ化アルブミンにより抗アポトーシス効果を付与した改良型臓器保存液の開発 (2012)
  • Author(s): 異島 優
  • Organizer: 第12回日本NO学会学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県）
• [Presentation] ヒト血清アルブミンドメインIIによるビリルビン尿中排泄促進作用 (2012)
  • Author(s): 小田切 優樹
  • Organizer: 第28回日本DDS学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
• [Presentation] 出血性ショックモデルラットにおける一酸化炭素付加型赤血球の肝地とクロームP450保護効果 (2012)
  • Author(s): 大柿 滋
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール（北海道）
• [Presentation] ESI-TOF/MSを用いたシステイン付加型ヒト血清アルブミンの検出と機能相関―慢性肝疾患の影響― (2012)
  • Author(s): 南雲 恒平
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール（北海道）
• [Presentation] 細胞型人工酸素運搬体 ヘモグロビン小胞体のカニクイザルへの大量投与の結果からみた実効性 (2012)
  • Author(s): 田口 和明
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール（北海道）
  • Invited
• [Presentation] 血小板代替物 H12 (ADP) リポソームの体内動態に及ぼす血小板減少症の影響 (2012)
  • Author(s): 丸山 徹
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール（北海道）
  • Invited
• [Presentation] ラット妊娠母体におけるヘモグロビン小胞体の胎盤通過性 (2012)
  • Author(s): 太田 英伸
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール（北海道）
• [Presentation] S-ニトロソ化に伴うα1-酸性糖タンパク質（AGP）の抗菌機能獲得と感染症治療への応用 (第34回生体膜と薬物の相互作用シンポジウム (2012)
  • Author(s): 渡辺 佳織
  • Organizer: 第34回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 京都大学薬学部（京都府）
• [Presentation] 尿毒症物質p-クレジル硫酸のレドックス特性と腎障害、心血管疾患発症機序解明 (2012)
  • Author(s): 宮本 洋平
  • Organizer: 第34回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 京都大学薬学部（京都府）
• [Presentation] 一酸化炭素付加赤血球によるクッパー細胞の不活化は輸血誘発肝チトクロームP450機能障害を保護する (2012)
  • Author(s): 大柿 滋
  • Organizer: 第34回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 京都大学薬学部（京都府）
• [Presentation] p-Cresyl sulfate causes renal tubular cell damage by inducing oxidative stress through the activation of NADPH oxidase. (2012)
  • Author(s): Watanabe H
  • Organizer: 49th ERA-EDTA Congress, Paris 2012.
  • Place of Presentation: Paris（France）
• [Presentation] A uremic toxin, 3-carboxy-4-methyl-5- propyl-2-furanpropionate accumulates in proximal tubular cells and induces cell damage through increasing oxidative stress. (2012)
  • Author(s): Miyamoto Y
  • Organizer: 49th ERA-EDTA Congress, Paris 2012.
  • Place of Presentation: Paris（France）
• [Presentation] Human serum albumin as a nanomedecine carrir. (2012)
  • Author(s): Otagiri M
  • Organizer: Nanoformulation 2012.
  • Place of Presentation: Fira Palace, Barcelona
  • Invited
• [Presentation] The potential of albumin for use as a nanomedicine carrier. (2012)
  • Author(s): Otagiri M.
  • Organizer: Montreal 2012 international forum on enviroment and medicine.
  • Place of Presentation: Montreal,Quebec,Canada
  • Invited
• [Presentation] Effect of genetically mannosylated-human serum albumin with nitric oxide on hepatic ischemia reperfusion injury (2011)
  • Author(s): Yu Ishima, Masaki Otagiri, et al.
  • Organizer: 4th Asia Pacific Regional ISSX Meeting
  • Place of Presentation: Tainan. Taiwan
• [Presentation] The uremic solute indoxyl sulfate acts as an antioxidant against superoxide anion radicals under normal-physiological conditions (2011)
  • Author(s): Yohei Miyamoto, Masaki Otagiri, et al.
  • Organizer: 4th Asia Pacific Regional ISSX Meeting
  • Place of Presentation: Tainan. Taiwan
• [Presentation] Characterization of structure and function of human serum albumin in patients with liver disease (2011)
  • Author(s): Nagumo K, Otagiri M, et al.
  • Organizer: 4th Asia Pacific Regional ISSX Meeting
  • Place of Presentation: Tainan. Taiwan
• [Presentation] Carbon monoxide binding red blood cells prevent cytochrome p450 degradation in resuscitation after hemorrhagic shock in a rat model (2011)
  • Author(s): Ogaki S, Otagiri M, et al.
  • Organizer: 4th Asia Pacific Regional ISSX Meeting
  • Place of Presentation: Tainan. Taiwan
• [Presentation] Improved therapeutic effect of thioredoxin by fusing to human serum albumin against ovalbumin-induced lung injury (2011)
  • Author(s): Ryota Tanaka, Masaki Otagiri, et al.
  • Organizer: 4th Asia Pacific Regional ISSX Meeting
  • Place of Presentation: Tainan. Taiwan
• [Presentation] Acute-phase protein alpha1-acid glycoprotein acquires anti-bacterial activity through S-nitrosylation by a nitric oxide-dependent mechanism (2011)
  • Author(s): Kaori Watanabe, Masaki Otagiri, et al.
  • Organizer: 4th Asia Pacific Regional ISSX Meeting
  • Place of Presentation: Tainan. Taiwan
• [Presentation] Albumin Fusion of Thioredoxin: Evaluation of Its Biological Activity for Potential Therapeutic Applications (2011)
  • Author(s): Masaki Otagiri
  • Organizer: 8th Retro metabolism Based Drug Design(招待講演)
  • Place of Presentation: Graz, Austria
• [Presentation] Quantitative Analysis of Cysteine-34 on the Anitioxidative Properties of Human Serum Albumin and its Physiological Significance in Hemodialysis Patients (2011)
  • Author(s): Makoto Anraku, Masaki Otagiri, et al.
  • Organizer: Helsinki Drug Research 2011 Congress
  • Place of Presentation: Helsinki, Finland
• [Presentation] Genetically engineered mannosylated-human serum albumin as a versatile carrier for liver-selective therapeutics (2011)
  • Author(s): Toru Maruyama, Masaki Otagiri, et al.
  • Organizer: 2011 AAPS Annual Meeting and Exposition
  • Place of Presentation: Chicago
• [Presentation] Involvement of hemoglobin beta-chain on plasma membrane in uptake of human alpha1-acid glycoprotein into liver (2011)
  • Author(s): Hisakazu Komori, Masaki Otagiri, et al.
  • Organizer: 2011 AAPS Annual Meeting and Exposition
  • Place of Presentation: Chicago
• [Presentation] A peptide moiety of human α1-acid glycoprotein is recognized by the hemoglobin (2011)
  • Author(s): Suenaga T, Otagiri M, et al.
  • Organizer: Asian Federation for Pharmaceutical Sciences 2011
  • Place of Presentation: Malaysia, Kuala Lumpur
• [Presentation] Interaction between p-cresyl sulfate and indoxyl sulfate during body disposition can influence their serum free concentrations in chronic kidney disease (2011)
  • Author(s): Watanabe H, Otagiri M, et al.
  • Organizer: Asian Federation for Pharmaceutical Sciences 2011
  • Place of Presentation: Malaysia, Kuala Lumpur
• [Presentation] Carbon monoxide bound red blood cells prevent alteration of hepatic cytochrome P450 activity after hemorrhagic shock and resuscitation (2011)
  • Author(s): Ogaki S, Otagiri M, et al.
  • Organizer: Asian Federation for Pharmaceutical Sciences 2011
  • Place of Presentation: Malaysia, Kuala Lumpur
• [Presentation] Human serum albumin-thioredoxin fusion protein with long blood retention property is effective in suppressing lung injury (2011)
  • Author(s): Ishima Y, Otagiri M, et al.
  • Organizer: Asian Federation for Pharmaceutical Sciences 2011
  • Place of Presentation: Malaysia, Kuala Lumpur
• [Presentation] Quantitative assessment of cysteinylated human serum albumin using ESI-TOF/MS and its clinical significance in chronic liver disease (2011)
  • Author(s): Nagumo K, Otagiri M, et al.
  • Organizer: Asian Federation for Pharmaceutical Sciences 2011
  • Place of Presentation: Malaysia, Kuala Lumpur
• [Book] HUMAN SERUM ALBUMIN (2011)
  • Author(s): Masaki Otagiri (Ed)
  • Total Pages: 300
  • Publisher: Sojo University Publishing Center