| Project/Area Number |
23659186
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Ehime University |
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Principal Investigator |
KITAZAWA Sohei 愛媛大学, 医学(系)研究科(研究院), 教授 (90186239)
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| Co-Investigator(Kenkyū-buntansha) |
KITAZAWA Riko 愛媛大学, 大学院・医学系研究科, 准教授 (00273780)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | DNA / メチル化シトシン / 組織細胞化学 / 遺伝子増幅 / in situ hybridization / エピジェネティクス / メチル化 / 組織化学 / PCR / 減数分裂 |
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| Research Abstract |
By making a ICON probe which specifically bound to methylated cytosine on chromosomal DNA in a sequence specific manner, a strong chemical cross-linking was made by osmium reaction. By so-called PRINS method, a DNA elongation reaction was established using bounded ICON probe on the specific chromosome. Modified nucleotides were incorporated during this elongation stem and visualized by a conventional histochemical techniques. By this easy, methyalation status of specific chromosome or DNA sequence could be visualized. So far we gathered a lot of basic and fundamental data. We are now currently trying to visualize a single-copy gene during spermatogenesis in mouse testis, and updating our protocol for more stable and reliable one.
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