Elucidation of molecular mechanisms involved in the immunomodulatory function of newly developed adipose tissue derived mesenchymal stem cells
Project/Area Number |
23659443
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Kidney internal medicine
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Research Institution | Nagoya University |
Principal Investigator |
MATSUO Seiichi 名古屋大学, 医学系研究科, 教授 (70190410)
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Co-Investigator(Kenkyū-buntansha) |
MARUYAMA Shoichi 名古屋大学, 医学(系)研究科(研究院), 准教授 (10362253)
AKIYAMA Shinichi 名古屋大学, 医学(系)研究科(研究院), 特任講師 (20500010)
OZAKI Takenori 名古屋大学, 医学部附属病院, 病院助教 (10452195)
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | 幹細胞 / 再生医療 / 免疫抑制 / 脂肪由来間葉系幹細胞 / 強皮症 / マクロファージ / メタボローム解析 |
Research Abstract |
We have proven that low serum cultured adipose derived mesenchymal stem cells (LASC) suppress T cell proliferation and induce polarization of macrophages into immunomodulatory M2 type, and that these effects of LASC were much stronger than high serum cultured adipose derived mesenchymal stem cells (HASC) or bone marrow derived mesenchymal stem cells (BM-MSC). Inthis study, we studied the mechanisms involved in the immunosuppressive functionof LASC by comparing with HASC or BM-MSC. We found by neutralization experiment and stimulation experiment that LASC derived PGE2 and IL-6 are necessary for the polarization of M2 macrophages, and that PGE2 function via EP4 receptor.
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Report
(3 results)
Research Products
(177 results)
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[Journal Article] Distribution of hydrogen sulfide (H(2)S)-producing enzymes and the roles of the H(2)S donor sodium hydrosulfide in diabetic nephropathy2013
Author(s)
Yamamoto J, Sato W, Kosugi T, Yamamoto T, Kimura T, Taniguchi S, Kojima H, Maruyama S, Imai E, Matsuo S, Yuzawa Y, Niki I
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Journal Title
Clin Exp Nephrol
Volume: 17(1)
Pages: 32-40
Related Report
Peer Reviewed
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[Journal Article] Transforming growth factor- β induces vascular endothelial growth factor-C expression leading to lymphangiogenesis in rat unilateral ureteral obstruction2012
Author(s)
Suzuki Y, Ito Y, Mizuno M, Kinashi H, Sawai A, Noda Y, Mizuno T, Shimizu H, Fujita Y, Matsui K, Maruyama S, Imai E, Matsuo S, Takei Y
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Journal Title
Kidney Int
Volume: 81(9)
Pages: 865-79
Related Report
Peer Reviewed
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[Journal Article]2012
Author(s)
Glomerular hyperfiltration in prediabetes and prehypertension. Okada R, Yasuda Y, Tsushita K, Wakai K, Hamajima N, Matsuo S
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Journal Title
Nephrol Dial Transplant
Volume: 27(5)
Pages: 1821-5
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Peer Reviewed
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[Journal Article] Combined Values of Serum Albumin, C-Reactive Protein and Body Mass Index at Dialysis Initiation Accurately Predicts Long-Term Mortality2012
Author(s)
Takahashi R, Ito Y, Takahashi H, Ishii H, Kasuga H, Mizuno M, Suzuki Y, Yuzawa Y, Maruyama S, Murohara T, Imai E, Matsuo S
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Journal Title
Am J Nephrol
Volume: 36(2)
Pages: 136-43
Related Report
Peer Reviewed
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[Journal Article] Complete remission within 2 years predicts a good prognosis after methylprednisolone pulse therapy in patients with IgA nephropathy2012
Author(s)
Tatematsu M, Yasuda Y, Morita Y, Sakamoto I, Kurata K, Naruse T, Yamamoto R, Tsuboi N, Sato W, Imai E, Matsuo s, Maruyama S
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Journal Title
Clin Exp Nephrol
Volume: 16(6)
Pages: 883-91
NAID
Related Report
Peer Reviewed
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[Journal Article] ANCA-associated systemic vasculitis in Japan: clinical features and prognostic changes2012
Author(s)
Yamagata K, Usui J, Saito C, Yamaguchi N, Hirayama K, Mase K, Kobayashi M, Koyama A, Sugiyama H, Nitta K, Wada T, Muso E, Arimura Y, Makino H, Matsuo s
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Journal Title
Clin Exp Nephrol
Volume: 16(4)
Pages: 580-8
NAID
Related Report
Peer Reviewed
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[Journal Article] Atrial natriuretic peptide ameliorates peritoneal fibrosis in rat peritonitis model2012
Author(s)
Kato H, Mizuno T, Mizuno M, Sawai A, Suzuki Y, Kinashi H, Nagura F, Maruyama S, Noda Y, Yamada K, Matsuo s, Ito Y
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Journal Title
Nephrol Dial Transplant
Volume: 27(2)
Pages: 526-36
Related Report
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[Journal Article] Transforming growth factor-β induces vascular endothelial growth factor-C expression leading to lymphangiogenesis in rat unilateral ureteral obstruction2012
Author(s)
Suzuki Y, Ito Y, Mizuno M, Kinashi H, Sawai A, Noda Y, Mizuno T, Shimizu H, Fujita Y, Matsui K, Maruyama S, Imai E, Matsuo S, Takei Y
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Journal Title
Kidney Int.
Volume: (印刷中)(未定)
Issue: 9
Pages: 865-79
DOI
Related Report
Peer Reviewed
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[Journal Article] Adipose-derived stromal cells cultured in a low-serum medium, but not bone marrow-derived stromal cells, impede xenoantibody production2011
Author(s)
Saka Y, Furuhashi K, Katsuno T, Kim H, Ozaki T, Iwasaki K, Haneda M, Sato W, Tsuboi N, Ito Y, Matsuo s, Kobayashi T, Maruyama S
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Journal Title
Xenotransplantation
Volume: 18(3)
Pages: 196-208
Related Report
Peer Reviewed
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[Journal Article] Growth factor Midkine is involved in the pathogenesis of renal injury induced by protein overload containing endotoxin2011
Author(s)
Kato K, Kosugi T, Sato W, Arata-Kawai H, Ozaki T, Tsuboi N, Ito I, Tawada H, Yuzawa Y, Matsuo s, Kadomatsu K, Maruyama S
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Journal Title
Clin Exp Nephrol
Volume: 15(3)
Pages: 346-54
NAID
Related Report
Peer Reviewed
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[Journal Article]
Author(s)
Low serum cultured adipose tissue-derived stromal cells ameliorate acute kidney injury in rats. Katsuno T, Ozaki T, Saka Y, Furuhashi K, Kim H, Yasuda K, Yamamoto T, Sato W, Tsuboi N, Mizuno M, Ito Y, Imai E, Matsuo S, Maruyama S
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Journal Title
Related Report
Peer Reviewed
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[Presentation] Low Serum Cultured Adipose-Derived Mesenchymal Stromal Cells Ameliorate Rat Model with Zymosan Induced Severe Peritonitis2012
Author(s)
Hangsoo Kim, Masashi Mizuno, Kazuhiro Furuhashi, Takayuki Katsuno, Takenori Ozaki, Kaoru Yasuda, Waichi Sato, Naotake Tsuboi, Yasuhiko Ito, Enyu Imai, Shoichi M aruyama Seiichi Matsuo
Organizer
45rd Annual Meeting of The American Society of Nephrology
Place of Presentation
San Diego, USA
Year and Date
2012-11-03
Related Report
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[Presentation] POTENTIAL OF ADIPOSE-DERIVED STEM CELLS FROM HUMAN/RAT/MOUSE AS A NEW TOOL FOR THE TREATMENT OF SYSTEMIC SCLEROSIS2012
Author(s)
Maruyama Shoichi, Shimizu, Asuka, Furuhashi, Kazuhiro, Ozaki Takenori, Kim, Hang Su, Abe, Tomoko, Katsuno, Takayuki, Tsuboi, Naotake, Matsuo seiichi
Organizer
International Society for Stem Cell Research 10th Annual Meeting
Place of Presentation
横浜
Year and Date
2012-06-13
Related Report
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[Presentation] Low Serum Cultured Adipose-derived Mesenchymal Stem Cells Ameliorate Crescentic Glomerulonephritis by Functional Polarization of Macrophages into Immunoregulatory M2 Phenotype2012
Author(s)
Kazuhiro Furuhashi, Naotake Tsuboi, Asuka Shimizu, Hangsoo Kim, Takayuki Katsuno, Yousuke Saka, Takenori Ozaki, Waichi Sato, Enyu Imai, Seiichi Matsuo,Shoichi Maruyama
Organizer
The Asia Pacific Meeting of Vasculitis and ANCA Workshop 2012(AP-VAS)
Place of Presentation
東京コンファレンスセンター
Year and Date
2012-03-29
Related Report
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[Presentation] Low Serum Cultured Adipose Derived Stem Cells Ameliorate Zymosan Induced Severe Rat Peritonitis Model2011
Author(s)
Hangsoo Kim, Masashi Mizuno,Kazuhiro Furuhashi, Takayuki Katsuno, Kaoru Yasuda, Takenori Ozaki, Waichi Sato, Naotake Tsuboi, Yasuhiko Ito, Enyu Imai, Shoichi M aruyama Seiichi Matsuo
Organizer
44rd Annual Meeting of The American Society of Nephrology
Place of Presentation
Philadelphia, PA
Year and Date
2011-11-11
Related Report
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[Presentation] Association of Adipokines with Cardiovascular Mortality in Patients on Hemodialysis2011
Author(s)
Shoichi Maruyama, Kaoru Yasuda, Hirotake Kasuga, Yoshinari Yasuda, Tomoki Kosugi, Waichi Sato, Naotake Tsuboi, Yasuhiko Ito, Enyu Imai, Seiichi Matsuo
Organizer
44rd Annual Meeting of The American Society of Nephrology
Place of Presentation
Philadelphia, PA
Year and Date
2011-11-10
Related Report
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[Presentation] Low Serum Cultured Adipose-Derived Mesenchymal Stem Cells, but Not Bone-Marrow Derived Mesenchymal Stem Cells, Ameliorate Rat Crescentic Glomerulonephritis by Functional Polarization of Macrophages into Immunoregulatory Phenotype2011
Author(s)
Kazuhiro Furuhashi, Naotake Tsuboi, PhD, Hangsoo Kim, Takayuki Katsuno,Takenori Ozaki, Waichi Sato, Enyu Imai, Seiichi Mats u o , S hoichi Maruyama
Organizer
44rd Annual Meeting of The American Society of Nephrology
Place of Presentation
Philadelphia, PA
Year and Date
2011-11-10
Related Report
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