| Project/Area Number |
23659486
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Kanazawa University |
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Principal Investigator |
NAKAO Shinji 金沢大学, 医学系, 教授 (70217660)
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| Co-Investigator(Kenkyū-buntansha) |
AKATSUKA Yoshiki 藤田保健衛生大学, 医学部, 准教授 (70333391)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 再生不良性貧血 / 6pLOH / 自己抗原 / CLT / HLA-B*40:02 / CTL / 肝炎関連再生不良性貧血 / HLA / 片親性二倍体 / 抗HLA抗体 |
|---|
| Research Abstract |
To determine the exact prevalence of HLA-A allele-lacking leukocytes (HLA-LLs) in patients with acquired aplastic anemia (AA), we improved the sensitivity of flow cytometry and examined peripheral blood leukocytes of patients with newly-diagnosed AA patients. HLA-LLs were detectable in 6 (28.6%) of 21 patients who were heterozygous with the HLA-A allele. The percentage of HLA-A allele-lacking granulocytes in the total granulocytes ranged from 3.9% to 61.1% (median 8.4%), which was lower than that in patients in remission after immunosuppressive therapy. Cytotoxic T cell (CTL) clones (A6) were successfully established from one of the patients possessing HLA-LLs by stimulating patient’s CD8+T cells with K562 cells transfected with HLA-B*40:02 gene. A6 CTL killed K562 in B*40:02 restricted manner but did not kill other cells including EB virus-transformed lymphoblastoid cell line and Jurkat T cell line transduced with B*40:02.
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