Analysis of human fetal hematopoiesis using human ES cells

• Project/Area Number: 23659533
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Embryonic/Neonatal medicine
• Research Institution: The University of Tokyo
• Principal Investigator: TSUJI Kohichiro 東京大学, 医科学研究所, 准教授 (50179991)
• Co-Investigator(Kenkyū-buntansha): OTSU Makoto 東京大学, 医科学研究所, 特任准教授 (30361330)
• Project Period (FY): 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: ヒトES細胞 / ヒト胎生期造血 / 間葉系幹細胞 / 造血前駆細胞

Research Abstract

We established a coculture system of human ES cells with autologous stromal cells derived from human ES cells to induce their differentiation into hematopoietic/blood cells, truly reflecting human fetal hematopoiesis. The human ES cell-derived stromal cells revealed a homologous phenotype of mesenchymal stem cells(MSCs), and hematopoietic/blood cells developed in the coculture with these human ES cell-derived MSCs were shown to originate from adult-type hematopoiesis. The present culture system using autologous human ES cell. derived MSCs can be useful for the analysis of human fetal hematopoiesis.

Research Products

• [Journal Article] The antigen CD34 : Amolecule displaying an implication in regenerative signal in hematopoietic stem cell (2012)
  • Author(s): Singh VK, Satija NK, Tripathi RP, et al.
  • Journal Title: STEM CELLS Trans. Med Volume: (in press)
  • Peer Reviewed
• [Journal Article] Unrelated cord blood transplantation after myeloablative conditioning regimen in adolescent and young adult patients with hematologic malignancies : a single institute analysis (2012)
  • Author(s): Ebihara, Y., et al.
  • Journal Title: Leuk.Res. Volume: 36 Issue: 2 Pages: 128-131
  • DOI: 10.1016/j.leukres.2011.09.016
  • Peer Reviewed
• [Journal Article] The antigen CD34 : A molecule displaying an implication in regenerative signal in hematopoietic stem cell (2012)
  • Author(s): Singh VK, et al
  • Journal Title: STEM CELLS Trans.Med. Volume: (in press)
  • Peer Reviewed
• [Journal Article] In vivo imaging visualizes discoid platelet aggregations without endothelium disruption and implicates contribution of inflammatory cytokine and integrin signaling (2011)
  • Author(s): S.Nishimura
  • Journal Title: Blood Volume: 119 Issue: 8 Pages: 128-131
  • DOI: 10.1182/blood-2011-09-381400
  • Peer Reviewed
• [Journal Article] Sero-prevalence of pandemic (H1N1) 2009 influenza A virus among schoolchildren and their parents in Tokyo, Japan (2011)
  • Author(s): Iwatsuki-Horimoto, K., et al.
  • Journal Title: Clin.Vaccine Immunol. Volume: 18 Issue: 5 Pages: 860-866
  • DOI: 10.1128/cvi.00428-10
  • Peer Reviewed
• [Journal Article] Frequent pathway mutations of splicing machinery in myelodysplasia (2011)
  • Author(s): Kenichi Yoshida
  • Journal Title: Nature Volume: 478 Issue: 7367 Pages: 64-69
  • DOI: 10.1038/nature10496
  • Peer Reviewed
• [Journal Article] Minimum requirement of donor cells to reduce the glycolipid storage following bone marrow transplantation in a murine model of Fabry disease (2011)
  • Author(s): Yokoi T, Kobayashi H, Shimada Y, Eto Y, Ishige N, Kitagawa T, Otsu M, Nakauchi H, Ida H, Ohashi T
  • Journal Title: J Gene Med Volume: 13 Issue: 5 Pages: 262-8
  • DOI: 10.1002/jgm.1566
  • Peer Reviewed
• [Journal Article] Intracellular estrogen receptor-binding fragment-associated antigen 9 exerts in vivo tumor-promoting effects via its coiled-coil region (2011)
  • Author(s): Maeyama, Y., Otsu, M., Kubo, S.
  • Journal Title: Int J Oncol Volume: 39 Pages: 41-49
  • DOI: 10.3892/ijo.2011.1026
  • Peer Reviewed
• [Journal Article] Heterozygous ITGA2B R995W mutation inducing constitutive activation of the {alpha}IIb{beta}3 receptor affects proplatelet formation and causes congenital macrothrombocytopenia (2011)
  • Author(s): Kunishima S, Kashiwagi H, Otsu M, Takayama N, Eto K, Onodera M, Miyajima Y, Takamatsu Y, Suzumiya J, Matsubara K, Tomiyama Y, Saito H
  • Journal Title: Blood Volume: 117 Issue: 20 Pages: 5479-5484
  • DOI: 10.1182/blood-2010-12-323691
  • Peer Reviewed
• [Journal Article] Growth promotion ofgenetically modified hematopoieticprogenitors using an antibody/c-Mpl chimera (2011)
  • Author(s): Kawahara, M., Chen, J., Sogo, T., Teng, J., Otsu, M., Onodera, M., Nakauchi, H., Ueda, H. and Nagamune, T.
  • Journal Title: Cytokine Volume: 55 Issue: 3 Pages: 402-408
  • DOI: 10.1016/j.cyto.2011.05.024
  • Peer Reviewed
• [Presentation] 誰でもわかる造血幹細胞の基礎知識 (2012)
  • Author(s): 辻浩一郎
  • Organizer: 第34回日本造血細胞移植学会
  • Place of Presentation: 大阪:大阪国際会議場
• [Presentation] 3回の同種臍帯血移植後の再発ALLに対し非寛解期でのT細胞非除去HLA半合致移植を施行した一例 (2012)
  • Author(s): 望月慎史, 他
  • Organizer: 第34回日本造血細胞移植学会
  • Place of Presentation: 大阪:大阪国際会議場
• [Presentation] 成人臍帯血移植患者におけるボリコナゾールとシクロスポリン併用療法の安全性評価 (2012)
  • Author(s): 前浩史, 他
  • Organizer: 第34回日本造血細胞移植学会
  • Place of Presentation: 大阪:大阪国際会議場
• [Presentation] 成人臍帯血移植患者におけるアンホテリシンBリポゾーム製剤投与の有効性と安全性の評価 (2012)
  • Author(s): 前浩史, 他
  • Organizer: 第34回日本造血細胞移植学会
  • Place of Presentation: 大阪:大阪国際会議場
• [Presentation] 3回の同種臍帯血移植後の再発ALLに対し非〓解期でのT細胞非除去HLA半合致移植を施行した一例 (2012)
  • Author(s): 望月慎史, 他
  • Organizer: 第34回日本造血細胞移植学会
  • Place of Presentation: 大阪:大阪国際会議場
• [Presentation] 動物細胞・動物血清を用いないヒトiPS細胞から血液細胞への分化誘導 (2011)
  • Author(s): 海老原康博
  • Organizer: 第21回日本サイトメトリー学会
  • Place of Presentation: 京都:京都国際交流会館
  • Year and Date: 2011-06-25
• [Presentation] Suppressed neutrophil development in hematopoiesis of induced pluripotent stem cells derivedfrom a severe congenital neutropenia patient with ELA2 mutation (2011)
  • Author(s): Hiramoto T., et al.
  • Organizer: 53^<rd> ASH Annual Meeting
  • Place of Presentation: San Diego, USA
• [Presentation] Matched HLA Haplotype Contributes to Reduce Sever Acute GVHD with Conserving GVL Effect in HLA-Mismatched Cord Blood Transplantation (2011)
  • Author(s): Takahashi S., et al.
  • Organizer: 53^<rd> ASH Annual Meeting
  • Place of Presentation: San Diego, USA
• [Presentation] Analysis of impaired hematopoiesis in Down syndrome using patient iPScells (2011)
  • Author(s): 望月慎史, 他
  • Organizer: 第73回日本血液学会学術集会
  • Place of Presentation: 名古屋:名古屋国際会議場
• [Presentation] GVL effect closely related with moderate a GVHD on CBT (2011)
  • Author(s): 河北敏郎, 他
  • Organizer: 第73回日本血液学会学術集会
  • Place of Presentation: 名古屋:名古屋国際会議場
• [Presentation] 患者由来iPS細胞を用いたダウン症候群における造血障害の解析 (2011)
  • Author(s): 望月慎史, 他
  • Organizer: 第114回日本小児科学会学術集会
  • Place of Presentation: 東京:高輪プリンスホテル
• [Presentation] Induction of hematopoiesis from human induced pluripotent stem cells(hiPSCs) in coculture with autologus hiPSC-derived mesenchymal stem cells under animal serum-free conditions (2011)
  • Author(s): Ebihara Y., et al.
  • Organizer: 9^<th> ISSCR Annual Meeting
  • Place of Presentation: Toronto, Canada
• [Presentation] 重症先天性好中球減少症患者由来のiPS細胞の樹立とその解析 (2011)
  • Author(s): 平本貴史, 他
  • Organizer: 第32回日本炎症・再生医学会
  • Place of Presentation: 京都:京都国際会館
• [Presentation] I Induction of hematopoiesis from human induced pluripotent stem cells (hiPSCs) in coculture with autologus hiPSC-derived mesenchymal stem cells under animal serum-free conditions (2011)
  • Author(s): Ebihara Y., et al
  • Organizer: 9^<th> ISSCR Annual Meeting
  • Place of Presentation: Toronto, Canada
• [Presentation] Suppressed neutrophil development in hematopoiesis of induced pluripotent stem cells derived from a severe congenital neutropenia patient with ELA2 mutation (2011)
  • Author(s): Hiramoto T., et al
  • Organizer: 53^<rd> ASH Annual Meeting
  • Place of Presentation: San Diego, USA
• [Presentation] GVL effect" closely related with moderate aGVHD on CBT (2011)
  • Author(s): 河北敏郎, 他
  • Organizer: 第73回日本血液学会学術集会
  • Place of Presentation: 名古屋:名古屋国際会議場
• [Book] 今日の小児治療指針(第15版) (2012)
  • Author(s): 辻浩一郎
  • Publisher: 医学書院
• [Book] Kerala Stem Cells in Medicine (2011)
  • Author(s): Ma, F., et al.
  • Publisher: Transworld Research Network
• [Book] Vienna Embryonic Stem Cells (2011)
  • Author(s): Ma, F., et al.
  • Publisher: Intech
• [Book] New York Human Embryonic and Induced Pluripotent Stem Cells (2011)
  • Author(s): Ma, F., et al.
  • Publisher: Springer Science
• [Book] Stem Cells in Medicine (2011)
  • Author(s): Ma, F., et al
  • Publisher: Transworld Research Network, Kerala
• [Book] Embryonic Stem Cells (2011)
  • Author(s): Ma, F., et al
  • Publisher: Intech, Vienna
• [Book] Human Embryonic and Induced Pluripotent Stem Cells (2011)
  • Author(s): Ma, F., et al
  • Publisher: Springer Science, New York
• [Remarks] 東京大学医科学研究所幹細胞治療研究センター幹細胞プロセシング分野
  • URL: http://stemcell-u-tokyo.org/scp/
• [Remarks] 東京大学医科学研究所幹細胞治療研究センター幹細胞プロセシング分野
  • URL: http://stemcell-u-tokyo.org/scp/
• [Patent(Industrial Property Rights)] 多能性幹細胞からの好酸球の製造方法 (2011)
  • Inventor(s): 辻浩一郎、馬峰、斎藤博久、松本健治、中畑龍俊
  • Industrial Property Rights Holder: 京都大学
  • Filing Date: 2011-12-02
  • Overseas
• [Patent(Industrial Property Rights)] ヒト由来の多能性幹細胞を分化させる方法 (2011)
  • Inventor(s): 辻浩一郎、海老原康博
  • Industrial Property Rights Holder: 東京大学
  • Filing Date: 2011-09-30
  • Overseas
• [Patent(Industrial Property Rights)] ヒト由来の多能性幹細胞を分化させる方法 (2011)
  • Inventor(s): 辻浩一郎、海老原康博
  • Industrial Property Rights Holder: 東京大学
  • Filing Date: 2011-09-30
  • Overseas
• [Patent(Industrial Property Rights)] 多能性幹細胞からの好酸球の製造方法 (2011)
  • Inventor(s): 辻浩一郎、馬峰、斎藤博久、松本健治、中畑龍俊
  • Industrial Property Rights Holder: 京都大学
  • Filing Date: 2011-12-02
  • Overseas