Development of stem cell therapy for epidermolysis bullosa model mice expressing humanized dermo-epidermal junctional protein
| Project/Area Number |
23659539
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Hokkaido University |
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Principal Investigator |
SHIMIZU Hiroshi 北海道大学, 大学院・医学研究科, 教授 (00146672)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Riichiro 北海道大学, 大学院・医学研究科, 准教授 (60344511)
FUJITA Yasuyuki 北海道大学, 北海道大学病院, 助教 (80374437)
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| Project Period (FY) |
2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 皮膚炎症・再生学 / 表皮水疱症 / VII型コラーゲン / 骨髄移植 / 骨髄由来細胞 / transdifferentiation |
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| Research Abstract |
The aim of the project is to investigate the effect of stem cell therapy for congenital severe skin disorder, recessive dystrophic epidermolysis bullosa(EB), using protein-humanized EB model mice. We performed bone marrow transplantation from GFP Tg mice to EB model mice lacking murine type VII collagen(COL7) and expressing incomplete/complete human COL7. Epithelized skin areas showed GFP+bone marrow-derived keratinocytes as many as 0. 2%. Murine COL7 mRNA and COL7 protein were also expressed in some mice, but the expression levels were low. We are now performing and analyzing another bone marrow transplantation model using HMGB1, CCL27 and CCL19, which are known to enhance transdifferentiation and recruitment from bone marrow cells to skin component cells.
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Report
(2 results)
Research Products
(4 results)
