Development of a monitoring system of in vivo cell dynamics for therapeutic evaluation of psychiatric disorders

• Project/Area Number: 23659562
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Psychiatric science
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: OUCHI Yasuomi 浜松医科大学, メディカルフォトニクス研究センター, 教授 (40436978)
• Co-Investigator(Kenkyū-buntansha): UEKI Takatoshi 浜松医科大学, 医学部, 准教授 (60317328)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 臨床精神分子遺伝学 / 神経幹細胞

Research Abstract

It has been reported that neural stem cells are variously changed in the process of not only normal neural growth but also neuronal degeneration occurring in neuropsychiatric disorders. Despite these findings, there was no study about the contribution of neural stem cells to the physiological and pathophysiological significance. Hence we tried to develop an in vivo monitoring system with which the dynamics of the stem cells can be visualized. Using positron emission tomography with a new gene-reporter system and amino acid transporter, we were able to make a new in vivo system for measuring live neural stem cells in vivo specifically.

Research Products

• [Journal Article] SPG3A-linked hereditary spastic paraplegia associated with cerebral glucose hypometabolism. (2013)
  • Author(s): Terada T, Kono S, Ouchi Y, Yoshida K, Hamaya Y, Kanaoka S, Miyajima H
  • Journal Title: Ann Nucl Med Volume: 27(3) Issue: 3 Pages: 303-8
  • DOI: 10.1007/s12149-012-0673-5
  • NAID: 10031168098
  • Peer Reviewed
• [Journal Article] Effect of subthalamic nucleus stimulation during exercise on the mesolimbocorital dopaminergic region in Parkinson's disease : A positron Emission Tomography Study (2013)
  • Author(s): Nozaki T, 他 8 人, Ouchi Y
  • Journal Title: J Cereb Blood Flow Metab Volume: 33 Issue: 3 Pages: 415-421
  • DOI: 10.1038/jcbfm.2012.183
• [Journal Article] Microglial activation in young adults with autism spectrum disorder (2013)
  • Author(s): Suzuki K
  • Journal Title: JAMA Psychiatry Volume: 70 Issue: 1 Pages: 49-58
  • DOI: 10.1001/jamapsychiatry.2013.272
  • Peer Reviewed
• [Journal Article] Alterations in α4β2 nicotinic receptors in cognitive decline in Alzheimer's etiopathology (2013)
  • Author(s): Okada H, Ouchi Y, Ueki T, 他 10 人
  • Journal Title: Brain Volume: 136(Pt10) Issue: 10 Pages: 3004-17
  • DOI: 10.1093/brain/awt195
  • Peer Reviewed
• [Journal Article] A review of the default mode network in aging and dementia based on molecular imaging (2012)
  • Author(s): Ouchi Y, Kikuchi M
  • Journal Title: Rev Neurosci Volume: 23(3) Issue: 3 Pages: 263-8
  • DOI: 10.1515/revneuro-2012-0029
• [Journal Article] In vivo mesolimbic D2/3 receptor binding predicts posttherapeutic clinical responses in restless legs syndrome: a positron emission tomography study (2012)
  • Author(s): Oboshi Y, Ouchi Y, Yagi S, Kono S, Nakai N, Yoshikawa E, Futatsubashi M, Terada T, Kim K, Harada K
  • Journal Title: J Cereb Blood Flow Metab Volume: 32(4) Issue: 4 Pages: 654-62
  • DOI: 10.1038/jcbfm.2011.201
  • Peer Reviewed
• [Journal Article] In vivo changes in microglial activation and amyloid deposits in brain regions with hypometabolism in Alzheimer's disease (2011)
  • Author(s): Yok oku r a M, Mo r i N, Yag i S, Yos h ik awa E, Kikuchi M, Yoshihara Y, Wakuda T, Sugihara G, Takebayashi K, Suda S, Iwata Y, Ueki T, Tsuchiya KJ, Suzuki K, Nakamura K, Ouchi Y
  • Journal Title: Eur J Nucl Med Mol Imaging Volume: 38 Issue: 2 Pages: 343-51
  • DOI: 10.1007/s00259-010-1612-0
• [Journal Article] Reduced acetylcholinesterase activity in the fusiform gyrus in adults with autism spectrum disorders (2011)
  • Author(s): Suzuki K, Sugihara G, Ouchi Y, Nakamura K, Tsujii M, Futatsubashi M, Iwata Y, Tsuchiya KJ, Matsumoto K, Takebayashi K, Wakuda T, Yoshihara Y, Suda S, Kikuchi M, Takei N, Sugiyama T, Irie T, Mori N
  • Journal Title: Arch Gen Psychiatry Volume: 68 Issue: 3 Pages: 306-13
  • DOI: 10.1001/archgenpsychiatry.2011.4
• [Journal Article] Effects of brain amyloid deposition and reduced glucose metabolism on the default mode of brain function in normal aging (2011)
  • Author(s): Kikuchi M, Hirosawa T, Yokokura M, Yagi S, Mori N, Yoshikawa E, Yoshihara Y, Sugihara G, Takebayashi K, Iwata Y, Suzuki K, Nakamura K, Ueki T, Minabe Y, Ouchi Y
  • Journal Title: Neurosci Volume: 31 Issue: 31 Pages: 11193-9
  • DOI: 10.1523/jneurosci.2535-11.2011
  • Peer Reviewed
• [Journal Article] New computer-aided diagnosis of dementia using positron emission tomography : brain regional sensitivity-mapping method (2011)
  • Author(s): Kakimoto A, Kamekawa Y, 他 5 人 Ouchi Y
  • Journal Title: PLoS One Volume: 6(9) Issue: 9 Pages: e25033-e25033
  • DOI: 10.1371/journal.pone.0025033
  • Peer Reviewed
• [Journal Article] Effects of brain amyloid deposition and reduced glucose metabolism on the default mode of brain function in normal aging. (2011)
  • Author(s): Kikuchi M, Hirosawa T, Yokokura M, Yagi S, Mori N, Yoshikawa E, Yoshihara Y, Sugihara G, Takebayashi K, Iwata Y, Suzuki K, Nakamura K, Ueki T, Minabe Y, Ouchi Y.
  • Journal Title: J Neurosci Volume: 31 Pages: 11193-11199
  • Peer Reviewed
• [Journal Article] Reduced acetylcholinesterase activity in the fusiform gyrus in adults with autism spectrum disorders (2011)
  • Author(s): Suzuki K, Sugihara G, Ouchi Y, Nakamura K, Tsujii M, Futatsubashi M, et al
  • Journal Title: Arch Gen Psychiatry Volume: 68 Pages: 306-313
  • Peer Reviewed
• [Journal Article] In vivo changes in microglial activation and amyloid deposits in brain regions with hypometabolism in Alzheimer’s disease. (2011)
  • Author(s): Yokokura M, Mori N, Yagi S, Yoshikawa E, Kikuchi M, Yoshihara Y, Wakuta T, Sugihara G, Takebayashi K, Suda S, Iwata Y, Ueki T, Tsuchiya KJ, Suzuki K, Nakamura K, Ouchi Y.
  • Journal Title: Eur J Nucl Med Mol Imaging Volume: 38 Pages: 343-351
  • Peer Reviewed
• [Journal Article] New computer-aided diagnosis of dementia using positron emission tomography: brain regional sensitivity-mapping method. (2011)
  • Author(s): Kakimoto A, Kamekawa Y, Ito S, Yoshikawa E, Okada H, Nishizawa S, Minoshima S, Ouchi Y.
  • Journal Title: PLoS One Volume: 6 Pages: 25033-25033
  • Peer Reviewed
• [Presentation] ミクログリアを治療標的とした神経変性疾患治療戦略の構築 (2014)
  • Author(s): 植木孝俊
  • Organizer: 第119回日本解剖学会総会・全国学術集会シンポジウム
  • Place of Presentation: 下野
  • Year and Date: 2014-03-29
• [Presentation] Effect of amyloid deposition on α4β2 nicotinic cholinergic system in Alzheimer's disease (2013)
  • Author(s): Ouchi Y, Terada T, Ueki T, 他7人
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都
  • Year and Date: 2013-06-22
• [Presentation] In vivo imaging of neuroinflammation using a new PET tracer [11C]-DPA713 (2013)
  • Author(s): Yokokura M, Ouchi Y, 他4人
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都
  • Year and Date: 2013-06-21
• [Presentation] NMR による多発性硬化症治療標的分子の生細胞内動態解析技術の創出 (2013)
  • Author(s): 植木孝俊
  • Organizer: 第54回日本神経学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2013-05-31
• [Presentation] The role of α4β2 nicotinic acetylcholine system in aging and Alzheimer's disease. (2013)
  • Author(s): Yasuomi Ouchi, Tatsuhiro Terada, Yumi Oboshi, Etsuji Yoshikawa, Masami Futatsubashi, Toshihiko Kanno, Mikako Ogawa, Yasuhiro Magata.
  • Organizer: Annual Meeting of the Organization for Human Brain Mapping
  • Place of Presentation: Seattle
• [Presentation] NG2 細胞のCD44 を分子標的とする分子イメージング技術の創出と、その多発性硬化症治療への応用 (2012)
  • Author(s): 植木孝俊
  • Organizer: 第53回日本神経学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2012-05-24
• [Presentation] Binding of α4β2 nicotinic acetylcholine receptor and cognition
  • Author(s): Ouchi Y, Terada T, Yagi S, Oboshi Y, Yoshikawa E, Futatsubashi M, Kanno T, Ogawa M, Magata Y.
  • Organizer: Annual Meeting of the Organization for Human Brain Mapping
  • Place of Presentation: Beijing