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Diagnostic bioimaging for acute cellular rejection using antibody probes in mouse liver transplantation

Research Project

Project/Area Number 23659607
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General surgery
Research InstitutionHokkaido University

Principal Investigator

MITSUCHIKA Hosoda  北海道大学, 大学病院, 講師 (40443931)

Co-Investigator(Kenkyū-buntansha) OZAKI Michitaka  北海道大学, 保健科学研究院, 教授 (80256510)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords肝移植 / 拒絶反応 / 生体イメージング
Research Abstract

We conducted this study to develop a new diagnostic method for allograft rejection in liver transplantation where infiltrating T-cells were quantified using antibody probes. We succeeded in inducing an acute cellular rejection in a mouse model of allogeneic liver transplantation. Immunohistologically, we confirmed that CD3 was strongly expressed on the infiltrating T-cells. However, a biological imaging after systemic injection of anti CD3 antibody probes could not detect the infiltrating T-cells. To deliver antibody probes to the infiltrating T-cells directly, we developed an extracorporeal perfusion machine for mouse liver and administered antibody probes to liver graft via the perfusion fluid. Even in this method, anti CD3 antibody probes could not detect the infiltrating T-cells.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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