Proteomic analysis of extracellular matrix proteins

• Project/Area Number: 23659727
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: The University of Tokushima
• Principal Investigator: SATO Nori 徳島大学, 大学病院, 医員 (00448333)
• Co-Investigator(Kenkyū-buntansha): YASUI Natsuo 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授 (00157984) ; HIGASHINO Kosaku 徳島大学, 大学院ヘルスバイオサイエンス研究部, 助教 (80380129) ; GODA Yuichiro 徳島大学, 病院, 助教 (70581148)
• Co-Investigator(Renkei-kenkyūsha): TANIGUCHI Hisaaki 徳島大学, 疾患酵素学研究センター, 教授 (10257636)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 細胞外マトリックス / コラーゲン / エラスチン / 質量解析 / 不溶性 / 電子顕微鏡 / 腰部脊柱管狭窄症

Outline of Final Research Achievements

Tendons and ligaments are mostly composed of extracellular matrix. These tissues are insoluble, and any study in whole proteomic analysis of them has not yet been reported. We succeeded in complete solubilization of human Achilles tendon and yellow ligament for HPLC-ESI-MS/MS. Our method will be applicable to investigation on protein composition of other extracellular matrix such as bone, cartilage, vessel, skin and so on, and it will be helpful to reveal the pathophysiology of pathological them.

Research Products

• [Journal Article] Mineral to matrix ratio determines biomaterial and biomechanical properties of rat femur-application of Fourier transform infrared spectroscopy (2011)
  • Author(s): Takata S
  • Journal Title: J Med Invest Volume: 58 Pages: 197-202
  • Peer Reviewed
• [Presentation] 腰部脊柱管狭窄症における黄色靭帯のタンパク解析 (2014)
  • Author(s): 合田有一郎、佐藤紀、谷口貴子、高田洋一郎、小坂浩史、酒井紀典、東野恒作、谷口寿章、西良浩一
  • Organizer: 第29回日本整形外科学会基礎学術集会
  • Place of Presentation: 城山観光ホテル（鹿児島県・鹿児島市）
  • Year and Date: 2014-10-09 – 2014-10-10
• [Presentation] 腰部脊柱管狭窄症における肥厚黄色靭帯のプロテオーム解析 (2013)
  • Author(s): 合田有一郎, 佐藤紀, 酒井紀典, 東野恒作, 加藤真介, 西良浩一
  • Organizer: 第120回中部日本整形外科災害外科学会学術集会
  • Place of Presentation: 和歌山県民文化会館・ホテルアバローム紀の国（和歌山県）
• [Presentation] Proteomic analysis of ligamentum flavum from lumbar spinal canal stenosis (2013)
  • Author(s): Yuichiro Goda, Nori Sato, Takako Taniguchi, Yoichiro Takata, Hirofumi Kosaka, Toshinori Sakai, Kosaku Higashino, Koichi Sairyo, Shinsuke Katoh, Hisaaki Taniguchi, Natsuo Yasui
  • Organizer: International Society for Study of the Lumbar Spine 2013
  • Place of Presentation: Fairmont Scottsdale Princess（Scottsdale, AZ, USA）
• [Presentation] Proteomic analysis of litamentum flavum (2013)
  • Author(s): Yuichiro Goda, Nori Sato, Takako Taniguchi, Yoichiro Takata, Hirofumi Kosaka, Toshinori Sakai, Kosaku Higashino, Koichi Sairyo, Shinsuke Katoh, Hisaaki Taniguchi, Natsuo Yasui
  • Organizer: 52nd International Spinal Cord Society
  • Place of Presentation: Halic Congress Center（Istanbul, Turkey）
• [Presentation] Quantitative Proteomic Analysis of Human Tendon and Ligament (2012)
  • Author(s): Nori Sato
  • Organizer: ORS 2012 Annual Meeting
  • Place of Presentation: San Francisco, CA, USA
• [Presentation] 不溶性細胞外マトリックスの可溶化と解析－ヒト腱・靭帯のプロテオーム解析－ (2011)
  • Author(s): 佐藤紀
  • Organizer: 第43回日本結合組織学会学術集会
  • Place of Presentation: 別府ビーコンプラザ（大分県）