Development of new regenerative therapy for central nerve system using the conditioned media derived from human dental pulp stem cells
| Project/Area Number |
23659914
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Nagoya University |
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Principal Investigator |
UEDA Minoru 名古屋大学, 医学系研究科, 教授 (00151803)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Akihito 名古屋大学, 大学院・医学系研究科, 准教授 (50244083)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ヒト歯髄幹細胞 / 培養上清 / 脊髄損傷 / 難治性中枢神経系疾患 / 神経再生 / 歯髄幹細胞 / ミクログリア / 抗炎症 |
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| Research Abstract |
We found that conditioned medium (CM) from stem cells derived from human exfoliated deciduous teeth (SHED), administered intrathecally into the severely injured adult rat SC, resulted in marked functional recovery. SHED-CM treatment inhibited SCI-induced apoptosis, preserved neural fibers and myelin sheaths, and promoted descending serotonergic raphespinal axon growth. Importantly, these pathophysiological recoveries were supported by a remarkable immunoregulatory function of SHED-CM, which converted the pro-inflammatory SCI conditions to a tissue repair/regenerating platform by modulating the microglia/macrophage phenotype. SHED-CM directly induced IL-10-producing M2 microglia synergistically with a major glial-scar extracellular matrix component, chondroitin sulphate proteoglycan, in vitro. Thus, SHED-CM may enable new stem-cell-based regenerative therapies for SCI that do not involve cell transplantation.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Human Dental Pulp-Derived Stem Cells Promote Locomotor Recovery after Complete Transection of the Rat Spinal Cord by Multiple Neuro-Regenerative Mechanisms2012
Author(s)
K. Sakai, A. Yamamoto, K. Matsubara, S. Nakamura, M. Naruse, M. Yamagata, K. Sakamoto, R. Tauchi, N. Wakao, S. Imagama, H. Hibi, K. Kadomatsu, N. Ishiguro, and M. Ueda
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Journal Title
The Journal of Clinical Investigation
Volume: Vol.122, No.1
Pages: 80-90
NAID
Related Report
Peer Reviewed
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