| Project/Area Number |
23659979
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontal dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
NAGATA Toshihiko 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (10127847)
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| Co-Investigator(Kenkyū-buntansha) |
INAGAKI Yuji 徳島大学, 病院, 助教 (50380019)
BANDO Mika 徳島大学, 病院, 医員 (10510000)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 模擬無重力 / 3D クリノスタット / 骨芽細胞 / 歯周病原性因子 / 歯周病 / 無重力 |
|---|
| Research Abstract |
This study investigated the effect of periodontopathic factors on osteoblastic differentiation of cultured rat bone-marrow cells under simulated microgravity. Experimental conditions were made up using 3D-clinostat for the simulated microgravity and lipopolysaccharide from P. gingivalis(P-LPS) for the periodontopathic factor. Osteoblastic differentiation of bone marrow cells was progressed by the microgravity, showing increases of bone-associated gene expression and bone-nodule formation in culture. These markers were inhibited by the addition of P-LPS. DNA-microarray analysis revealed an increase of gene expression of caspase, a molecule associated with endoplasmic reticulum stress. These results suggest that P-LPS may affect on osteoblastic differentiation under the microgravity condition.
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