in vitro artificial extracellular microenvironments to investigate the mechanisms of cell regulations

• Project/Area Number: 23681028
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Microdevices/Nanodevices
• Research Institution: Kyoto University
• Principal Investigator: KAMEI Ken-ichiro 京都大学, 物質-細胞統合システム拠点, 特定拠点助教 (00588262)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000); Fiscal Year 2012: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000); Fiscal Year 2011: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
• Keywords: マイクロバイオシステム / ヒト ES/iPS 細胞 / 細胞外微小環境 / ヒト多能性幹細胞 / マイクロ流体デバイス / ナノファイバー / 微細加工技術 / 細胞外微小環境因子 / ハイスループット技術

Research Abstract

Cells in a body or tissues are placed in cellular microenvironments, and regulated their functions as well as fate decision in a highly regulated manner. In this study, I tried to create artificial cellular microenvironments with microfluidic and nanotechnologies to establish a new regulatory system for human pluripotent stem cells (hPSCs). The obtained results and findings will contribute the advancement of regenerative medicine and drug development using hPSCs.

Research Products

• [Journal Article] Integration of porous coordination polymers and gold nano-rods into core-shell mesoscopic composites towards light-induced molecular release (2013)
  • Author(s): Kira Khaletskaya, Julien Reboul, Mikhail Meilikhov, Masashi Nakahama, Stéphane Diring, Masahiko Tsujimoto, Seiji Isoda, Franklin Kim, Ken-ichiro Kamei, Roland A. Fischer, Susumu Kitagawa and Shuhei Furukawa
  • Journal Title: Journal of the American Chemical Society Volume: 135 Issue: 30 Pages: 10998-11005
  • DOI: 10.1021/ja403108x
  • Peer Reviewed
• [Journal Article] Phenotypic and transcriptional modulation of human pluripotent stem cells induced by nano/microfabrication materials (2013)
  • Author(s): K. Kamei, Y. Hirai, Y. Makino, M. Yoshioka, Q. Yuan, M. Nakajima, Y. Chen, and O. Tabata
  • Journal Title: Advanced Healthcare Materials Volume: 2(2) Issue: 2 Pages: 287-291
  • DOI: 10.1002/adhm.201200283
  • Peer Reviewed
• [Journal Article] Fabrication of gelatin nanopatterns for cell culture studies (2013)
  • Author(s): Sisi Li
  • Journal Title: Microelectron. Eng. Volume: in press Pages: 70-74
  • DOI: 10.1016/j.mee.2013.01.053
  • Peer Reviewed
• [Journal Article] Integrated and diffusion-based micro-injectors for open access cell assays (2011)
  • Author(s): X.Li, L.Liu, L.Wang, K.Kamei, Q.Yuan, F.Zhang, J.Shi, A.Kusumi, M.Xie, Z.Zhao, Y.Chen
  • Journal Title: Lab Chip Volume: 11 Issue: 15 Pages: 2612-2617
  • DOI: 10.1039/c1lc20258h
  • Peer Reviewed
• [Journal Article] Delivery of Intact Transcription Factor by Using Self-Assembled Supramolecular Nanoparticles (2011)
  • Author(s): Y.Liu, H.Wang, K.Kamei, M.Yan, K.-J.Chen, Q.Yuan, L.Shi, Y.Lu, H.-R.Tseng
  • Journal Title: Angew.Chem.Int.Ed.Engl. Volume: 50 Issue: 13 Pages: 3058-3062
  • DOI: 10.1002/anie.201005740
  • Peer Reviewed
• [Journal Article] Cutting-edge microfabricated biomedical tools for human pluripotent stem cell research
  • Author(s): K. Kamei
  • Journal Title: Journal of Laboratory Automation Volume: (in press)
• [Presentation] Gelatin nanofibrous scaffold enables maintaining human ES/iPS cells for long-term period (2013)
  • Author(s): K. Kamei
  • Organizer: The 1st Bristol-Kyoto Symposium 2013
  • Place of Presentation: Bristol, UK
  • Invited
• [Presentation] Evaluation of Negative Photoresists on Phenotypes of Human Induced Pluripotent Stem Cells (hiPSCs) (2012)
  • Author(s): K. Kamei, Y. Hirai, Y. Makino, L. Liu, Q. Yuan, M. Yoshioka, Y. Chen, O. Tabata
  • Organizer: The IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2012-03-08
• [Presentation] Evaluation of Negative Photoresists on Phenotypes of Human Induced Pluripotent Stem Cells (hiPSCs) (2012)
  • Author(s): K.Kamei, Y.Hirai, Y.Makino, L.Liu, Q.Yuan, M.Yoshioka, Y.Chen, O.Tabata
  • Organizer: The IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS)
  • Place of Presentation: 京都大学
  • Year and Date: 2012-03-08
• [Presentation] Phenotypic modulation of pluripotent stem cells (PSCs) induced by microfabrication materials. (2012)
  • Author(s): K. Kamei, Y. Hirai, Y. Makino, M. Yoshioka, M. Nakajima, Q. Yuan, Y. Chen and O. Tabata
  • Organizer: The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS)
  • Place of Presentation: Okinawa, Japan
• [Presentation] 錯体フレームワークを用いた光駆動型生体ガス放出材料 (2012)
  • Author(s): 金 致源・Diring, Stephane・亀井 謙一郎・北川 進・古川 修平
  • Organizer: 日本化学会第92 春季年会
• [Presentation] 錯体フレームワークと生体適合材料の融合による機能性細胞外マトリクスの構 築 (2012)
  • Author(s): 中浜 雅士・Reboul, Julien・亀井 謙一郎・北川 進・古川 修平
  • Organizer: 日本化学会第92 春季年会
• [Presentation] Gelatin nanofibrous scaffold enables expanding human pluripotent stem cells for long-term period (2012)
  • Author(s): K. Kamei
  • Organizer: 6th Nanobiotechnology Symposium
  • Place of Presentation: 京都大学、京都
  • Invited
• [Presentation] Artificial regulatory microenvironments (niche) for controlling cell function (2012)
  • Author(s): K. Kamei
  • Organizer: IEEE Nanotechnology Material and Devices Conference (IEEE-NMDC)
  • Place of Presentation: Hawaii, USA
  • Invited
• [Presentation] Phenotypic modulation of pluripotent stem cells by microfabrication materials (2012)
  • Author(s): K. Kamei, Y. Hirai, Y. Makino, M. Yoshioka, M. Nakajima, Q. Yuan, Y. Chen and O. Tabata
  • Organizer: 10th International Society of Stem Cell Research (ISSCR) Annual Meeting
  • Place of Presentation: 横浜
• [Presentation] Phenotypic modulation of pluripotent stem cells (PSCs) induced by microfabrication materials (2012)
  • Author(s): K. Kamei, Y. Hirai, Y. Makino, M. Yoshioka, M. Nakajima, Q. Yuan, Y. Chen and O. Tabata
  • Organizer: The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS)
  • Place of Presentation: 沖縄
• [Presentation] 錯体フレームワークを用いた光駆動型生体ガス放出材料 (2012)
  • Author(s): 金致源・Diring, Stephane・亀井謙一郎・北川進・古川修平
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 慶應義塾大学
• [Presentation] 錯体フレームワークと生体適合材料の融合による機能性細胞外マトリクスの構築 (2012)
  • Author(s): 中浜雅士・Reboul, Julien・亀井謙一郎・北川進・古川修平
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 慶應義塾大学
• [Presentation] Micro-/nano-engineered cellular environments for controlling cellular functions (2011)
  • Author(s): 亀井謙一郎
  • Organizer: Seoul NanoHealth Symposium 2011
  • Place of Presentation: Yonsei University(韓国)(招待講演)
  • Year and Date: 2011-11-17
• [Presentation] Micro-/nano-engineered cellular environments for controlling cellular functions (2011)
  • Author(s): 亀井謙一郎
  • Organizer: AMS-iCeMS Joint Workshop 2011
  • Place of Presentation: Postech(韓国)(招待講演)
  • Year and Date: 2011-11-16
• [Presentation] Micro-/nano-engineered cellular environments for controlling cellular functions (2011)
  • Author(s): 亀井謙一郎
  • Organizer: MRC-CRM & iCeMS "Next Generation Stem Cells : Tools and Technologies Symposium"
  • Place of Presentation: The University of Edinburg, Edinburg(イギリス)(招待講演)
  • Year and Date: 2011-07-25
• [Presentation] Electrospun nanofibers for creating artificial cellular microenvironments for culturing mouse embryonic stem cells (2011)
  • Author(s): L. Liu, Q. Yuan, J. Shi, X. Li, D. Jung, L. Wang, K. Yamauchi, N. Nakatsuji, K. Kamei, Y. Chen
  • Organizer: 2nd Nanotoday Conference
  • Place of Presentation: Hawaii, USA
• [Presentation] Quantitative Cytological Feature Analysis by Microfluidic Image Cytometry Reveals Phenotypic Differences among Human Pluripotent Stem Cell Lines (2011)
  • Author(s): K. Kamei, M. Ohashi, N. A. Graham, Y. Chen, A. T. Clark, O. N. Witte, T. G. Graeber, A. D. Pyle and H.-R. Tseng
  • Organizer: The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS)
  • Place of Presentation: Seattle, WA, USA
• [Presentation] Quantitative Cytological Feature Analysis by Microfluidic Image Cytometry Reveals Phenotypic Differences among Human Pluripotent Stem Cell Lines (2011)
  • Author(s): K. Kamei, M. Ohashi, N. Graham, L. Liu, Q. Yuan, A. T. Clark, O.N. Witte, T. G. Graeber, A. D. Pyle, N. Nakatsuji, Y. Chen and H.-R. Tseng
  • Organizer: 9th International Society of Stem Cell Research (ISSCR) Annual Meeting
  • Place of Presentation: Toronto, Canada
• [Presentation] Electrospun nanofibers for creating artificial cellular microenviroments for culturing mouse embryonic stem cells (2011)
  • Author(s): L.Liu, Q.Yuan, J.Shi, X.Li, D.Jung, L.Wang, K.Yamauchi, N.Nakatsuji, K.Kamei, Y.Chen
  • Organizer: 2nd Nanotoday Conference
  • Place of Presentation: ハワイ(アメリカ)
• [Presentation] mESC and hiPSC Proliferation on Negative Photoresists for Microfluidics (2011)
  • Author(s): Y.Hirai, K.Kamei, Y.Makino, L.Liu, Q.Yuan, Y.Chen, O.Tabata
  • Organizer: Eurosensors XXV
  • Place of Presentation: アテネ(ギリシャ)
• [Presentation] Quantitative Cytological Feature Analysis by Microfluidic Image Cytometry Reveals Phenotypic Differences among Human Pluripotent Stem Cell Lines (2011)
  • Author(s): K.Kamei, M.Ohashi, N.A.Graham, Y.Chen, A.T.Clark, O.N.Witte, T.G.Graeber, A.D.Pyle, H.-R.Tseng
  • Organizer: microTAS2011
  • Place of Presentation: シアトル(アメリカ)
• [Presentation] Evaluation of Negative Photoresists for Culturing Mouse and Human Pluripotent Stem Cells (2011)
  • Author(s): K.Kamei, Y.Hirai, Y.Makino, L.Liu, Q.Yuan, Y.Chen, O.Tabata
  • Organizer: The 28th SENSOR SYMPOSIUM on Sensors, Micromachines and Application Systems, IEEJ Sensors and Micromachine Society
  • Place of Presentation: タワーホール船堀(東京都)
• [Presentation] Nano/microengineering approach to investigate and control stem cell function
  • Author(s): K. Kamei
  • Organizer: Monash University
  • Place of Presentation: Monash University, Clayton, Australia
  • Invited
• [Presentation] Gelatin nanofibrous scaffold to maintain human ES/iPS cells for long-term period
  • Author(s): K. Kamei
  • Organizer: Joint SCA and iCeMS Symposium
  • Place of Presentation: University of Melbourne, Melbourne, Australia
  • Invited
• [Presentation] Nanofiber scaffolds to maintain human pluripotent stem cells under feeder-free conditions
  • Author(s): K. Kamei
  • Organizer: Mini-symposium of WPI-iCeMS and Edinburg University
  • Place of Presentation: Edinburg University, Edinburg, UK
  • Invited
• [Remarks]
  • URL: http://www.chen.icems.kyoto-u.ac.jp/
• [Remarks]
  • URL: http://ken1kamei.wordpress.com/
• [Remarks] 亀井 謙一郎、古川 修平「ナノバイオテクノロジーが創出する学際融合研究の場: 6th Annual Symposium on Nanobiotechnology 」実験医学 , 31(3), 592-595 (2013)
• [Remarks] 亀井 謙一郎「ナノメディシン:ナノ粒子の臨床応用に向けて」実験医学, 30(12), 1928-1929 (2012)
• [Remarks] Ken-ichiro Kamei @ iCeMS, Kyoto University
  • URL: http://ken1kamei.wordpress.com
• [Remarks] Chen Group, iCeMS, Kyoto University
  • URL: http://www.chen.icems.kyoto-u.ac.jp
• [Remarks]
  • URL: http://www.chen.icems.kyoto-u.ac.jp/
• [Remarks]
  • URL: http://kenlkamei.wordpress.com/
• [Patent(Industrial Property Rights)] ファイバー・オン・ファイバーを用いた多能性幹細胞の3次元 (2013)
  • Inventor(s): 亀井 謙一郎、中辻 憲夫、劉 莉
  • Industrial Property Rights Holder: 京都大学
  • Industrial Property Number: 2013-117242
  • Filing Date: 2013-06-03
• [Patent(Industrial Property Rights)] 多能性幹細胞の培養方法及びそのための基材 (2012)
  • Inventor(s): 陳 勇、亀井 謙一郎、劉 莉
  • Industrial Property Rights Holder: 京都大学
  • Industrial Property Number: 2012-127580
  • Filing Date: 2012-06-04
• [Patent(Industrial Property Rights)] 多能性幹細胞の培養方法及びそのための基材 (2012)
  • Inventor(s): 陳 勇、亀井 謙一郎、劉 莉
  • Industrial Property Rights Holder: 京都大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-127580
  • Filing Date: 2012-06-04