Structural basis of the autophagy-specific E1 enzyme Atg7
| Project/Area Number |
23687012
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Microbial Chemistry Research Foundation |
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Principal Investigator |
NODA Nobuo 公益財団法人微生物化学研究会, 微生物化学研究所, 主席研究員 (40396297)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2011: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Keywords | オートファジー / Atg7 / E1酵素 / 構造生物学 / 結晶構造 / Atg3 / Atg12-Atg5 / E2酵素 / E3酵素 / Atg10 / Atg8 |
|---|
| Research Abstract |
The crystal structure of Atg7, the E1 enzyme specific to autophagy, has been determined alone and in complex with various Atg factors. Using the structural information, mutational analyses were performed, which revealed that Atg7 recognizes ubiquitin-like proteins and E2 enzymes using unique structures that were not observed in other E1 enzymes and that Atg7 catalyzes the conjugation reaction between ubiquitin-like proteins and E2 enzymes using a novel mechanism that has never been observed in other E1 enzymes. Since the reactions catalyzed by Atg7 are essential only to autophagy, the obtained knowledge will contribute to the development of novel compounds that specifically regulate autophagy.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] Atg12-Atg5 conjugate enhances E2 activity of Atg3 by rearranging its catalytic site2013
Author(s)
Sakoh-Nakatogawa, M., Matoba, K., Asai, E., Kirisako, H., Ishii, J., Noda, N. N., Inagaki, F., Nakatogawa, H. and Ohsumi, Y
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Journal Title
Nature Structural & Molecular Biology
Volume: Vol.20
Issue: 4
Pages: 433-439
DOI
Related Report
Peer Reviewed
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[Journal Article] Noncanonical recognition and UBL loading of distinct E2s by autophagy-essential Atg7.2012
Author(s)
Yamaguchi M, Matoba K, Sawada R, Fujioka Y, Nakatogawa H, Yamamoto H, Kobashigawa Y, Hoshida H, Akada R, Ohsumi Y, Noda NN, Inagaki F.
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Journal Title
Nat. Struct. Mol. Biol.
Volume: 19
Issue: 12
Pages: 1250-1256
DOI
Related Report
Peer Reviewed
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[Journal Article] Structural insights into Atg10-mediated formation of the autophagy-essential Atg12-Atg5 conjugate2012
Author(s)
Yamaguchi, M., Noda, N. N., Yamamoto, H., Shima, T., Kumeta, H., Kobashigawa, Y., Akada, R., Ohsumi, Y. and Inagaki, F
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Journal Title
Structure
Volume: Vol.20
Issue: 7
Pages: 1244-1254
DOI
Related Report
Peer Reviewed
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[Journal Article] Structural basis of Atg8 activation by a homodimeric E1, Atg72011
Author(s)
Noda, N.N., Satoo, K., Fujioka, Y., Kumeta, H., Ogura, K., Nakatogawa, H., Ohsumi, Y., Inagaki, F.
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Journal Title
Molecular Cell
Volume: 44
Pages: 462-475
Related Report
Peer Reviewed
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