| Budget Amount *help |
¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
Post-transcriptional modifications at the tRNA anticodon loop are essential for precise decoding of the genetic code. The modification defects in mitochondrial tRNA are the main cause of the mitochondrial dysfunction. In this study, I determined the crystal structure of the tRNA modification enzyme, which is responsible for synthesizing the cyclic form of N6-threonylcarbamoyladenosine, as part of elucidating the tRNA modification mechanisms. Together with the structure-based mutational experiments, the amino acid residues crucial for the reaction were also identified.
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