| Budget Amount *help |
¥28,470,000 (Direct Cost: ¥21,900,000、Indirect Cost: ¥6,570,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
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| Research Abstract |
To understand the molecular mechanism of biomolecular reactions, the dynamics of the molecules along the reaction have to be revealed by the real-time measurements. However, the yield of protein is usually very low, ug-mg, and the time-resolved measurements is hard to apply for many biomolecular reactions because of the relatively large consumption. In this research, the time-resolved spectroscopies applicable to the low-yield protein samples were developed and applied to observe the folding, membrane transport and enzymatic reactions.
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