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Identification of genes involved in flower longevity

Research Project

Project/Area Number 23688004
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Horticulture/Landscape architecture
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

YAMADA Tetsuya  東京農工大学, (連合)農学研究科(研究院), 准教授 (20422511)

Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2011: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Keywords細胞老化 / プログラム細胞死 / 花弁老化 / 花持ち性 / アサガオ / 花弁展開 / 次世代シーケンサー / トランスクリプトーム / 開花 / 暗期応答 / 概日時計 / 品種間差異 / エチレン感受性 / 種子生産 / トランスクリプトーム解析 / アセンブラ / 参照配列 / 次世代シークエンサー / de novoアセンブル / 暗期応答遺伝子 / RNAi法 / エチレン / 切り花
Outline of Final Research Achievements

In this study, we performed comparative expression analysis by using RNA-Seq and RT-qPCR methods in the petals of morning glory and revealed that transcription factor gene, InNAP, and ethylene-related genes were associated with the strain difference of flower longevity. We also confirmed that several genes involved in the control of petal senescence by using transgenic plants which reduced expression of 14-3-3 protein gene, InPSR42. In addition, we detected dark-responsive genes, DR03 and DR11, involved with the determination of petal opening time of morning glory by SSH methods.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Products Report
  • Research Products

    (16 results)

All 2016 2015 2014 2013 2012 2011

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 1 results) Presentation (11 results)

  • [Journal Article] Morphological changes in senescing petal cells and the regulatory mechanism of petal senescence2016

    • Author(s)
      Kenichi Shibuya, Tetsuya Yamada, Kazuo Ichimura
    • Journal Title

      Journal of Experimental Botany

      Volume: 67 Issue: 20 Pages: 5909-5918

    • DOI

      10.1093/jxb/erw337

    • Description
      In press
    • Related Report
      Products Report
    • Peer Reviewed
  • [Journal Article] InPSR42, a putative 14-3-3 protein, regulates petal opening and senescence in Japanese morning glory2015

    • Author(s)
      Hanako Ono, Kazuo Ishii, Toshinori Kozaki, Keiichi Shimizu, Kenichi Shibuya, Kazuo Ichimura, Motoki Kanekatsu, Tetsuya Yamada
    • Journal Title

      Acta Horticulturae

      Volume: 印刷中

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Removal of redundant contigs from de novo RNA-Seq assemblies via homology search improves accurate detection of differentially expressed genes2015

    • Author(s)
      Hanako Ono, Kazuo Ishii, Toshinori Kozaki, Isao Ogiwara, Motoki Kanekatsu, Tetsuya Yamada
    • Journal Title

      BMC Genomics

      Volume: 16 Issue: 1 Pages: 1031-1031

    • DOI

      10.1186/s12864-015-2247-0

    • Related Report
      Products Report
    • Peer Reviewed
  • [Journal Article] Length of the dark period affects flower opening and the expression of circadian-clock associated genes as well as xyloglucan endotransglucosylase/hydrolase genes in petals of morning glory (Ipomoea nil)2014

    • Author(s)
      Yoshihito Shinozaki, Ryusuke Tanaka, Hanako Ono, Isao Ogiwara, Motoki Kanekatsu, Wouter G. van Doorn, Tetsuya Yamada
    • Journal Title

      Plant Cell Reports

      Volume: 33 Pages: 1121-1131

    • DOI

      10.1007/s00299-014-1601-z

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of an AtNAP gene homolog in senescing morning glory (Ipomoea nil) petals of two cultivars with a different flower life span2014

    • Author(s)
      Yoshihito Shinozaki, Toshimitsu Tanaka, Isao Ogiwara, Motoki Kanekatsu, Wouter G. van Doorn, Tetsuya Yamada
    • Journal Title

      Journal of Plant Physiology

      Volume: 171 Issue: 8 Pages: 633-638

    • DOI

      10.1016/j.jplph.2014.01.008

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] RNA-Seqリードのde novo assemblyで構築したコンティグから相同性検索により冗長性を除去すると発現変動遺伝子の検出精度は向上する2016

    • Author(s)
      小野華子, 石井一夫, 古崎利紀, 荻原勲, 金勝一樹, 山田哲也
    • Organizer
      園芸学会平成28年度春季大会
    • Place of Presentation
      東京農業大学(神奈川県厚木市)
    • Year and Date
      2016-03-26
    • Related Report
      Products Report
  • [Presentation] アサガオの花弁展開に関する細胞・分子生物学的研究2015

    • Author(s)
      田中龍佑,篠崎良仁,山田哲也
    • Organizer
      第8回アサガオ研究集会
    • Place of Presentation
      筑波大学(茨城県つくば市)
    • Year and Date
      2015-03-06 – 2015-03-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 開花時のアサガオ花弁で部位特異的な発現変動を示す遺伝子群の同定2014

    • Author(s)
      田中龍佑,篠崎良仁,小野華子,渋谷健市,市村一雄,金勝一樹,山田哲也
    • Organizer
      園芸学会平成26年度秋季大会
    • Place of Presentation
      佐賀大学(佐賀県佐賀市)
    • Year and Date
      2014-09-26 – 2014-09-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] InPSR42, a putative 14-3-3 protein, regulates petal opening and senescence in Japanese morning glory2014

    • Author(s)
      Hanako Ono, Kazuo Ishii, Toshinori Kozaki, Keiichi Shimizu, Kenichi Shibuya, Kazuo Ichimura, Motoki Kanekatsu, Tetsuya Yamada
    • Organizer
      The 29th International Horticultural Congress
    • Place of Presentation
      Brisbane Convention & Exhibition Centre (Queensland, Australia)
    • Year and Date
      2014-08-17 – 2014-08-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 花持ち性に差異のあるアサガオ2品種間の花弁に見出されたエチレン感受性の違い2014

    • Author(s)
      石井裕也、田中俊光、篠崎良仁、金勝一樹、山田哲也
    • Organizer
      日本育種学会第125回
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2014-03-20 – 2014-03-22
    • Related Report
      2013 Annual Research Report
  • [Presentation] 花持ち性に差異のあるアサガオ2系統間の花弁における次世代シーケンサーを用いた比較トランスクリプトーム解析2013

    • Author(s)
      小野華子、石井一夫、古崎利紀、 山田哲也、金勝一樹
    • Organizer
      園芸学会平成25年度秋季大会
    • Place of Presentation
      岩手大学(岩手県盛岡市)
    • Year and Date
      2013-09-20 – 2013-09-22
    • Related Report
      2013 Annual Research Report
  • [Presentation] 暗期応答性を示すアサガオの開花に伴う花弁でのエクスパンシンおよびエンド型キシログルカン転移酵素/加水分解酵素遺伝子の発現変動2013

    • Author(s)
      田中龍佑、篠崎良仁、小野華子、山田哲也、金勝一樹
    • Organizer
      園芸学会平成25年度秋季大会
    • Place of Presentation
      岩手大学(岩手県盛岡市)
    • Year and Date
      2013-09-20 – 2013-09-22
    • Related Report
      2013 Annual Research Report
  • [Presentation] メラトニン処理がアサガオの葉や花弁の老化に及ぼす影響2013

    • Author(s)
      小野華子
    • Organizer
      園芸学会平成25年度春季大会
    • Place of Presentation
      東京農工大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] アサガオの開花および花弁老化の制御に関与する暗期応答遺伝子の探索2012

    • Author(s)
      篠崎良仁
    • Organizer
      園芸学会平成24年度春季大会
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] アサガオ花弁の新規mRNAアセンブルによる次世代シーケンス解析用参照配列の構築2012

    • Author(s)
      小野華子
    • Organizer
      園芸学会平成24年度秋季大会
    • Place of Presentation
      福井県立大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 14-3-3タンパク質コード遺伝子In42はアサガオ花弁のプログラム細胞死に関与する2011

    • Author(s)
      小野華子
    • Organizer
      園芸学会平成23年度秋季大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2011-09-26
    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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