| Budget Amount *help |
¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2012: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2011: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
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| Research Abstract |
This study was evaluated the functional mechanism of NADPH oxidase (Nox) during host penetration. In black spot disease, two Nox homologues, NoxA and NoxB are present and only the NoxB was involved in host penetration. Therefore, we evaluated the expression pattern and localization of Nox(s). The expression of Nox were induced during spore germination. NoxB protein was localized at the appressorium. The disruption of noxB resulted in disorder of actin accumulation at the appressorium. Moreover, NoxB was interacted with RacA. The disruption mutant of racA was affected multiple hyphal growth stages and lost pathogenicity.
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