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Molecular basis and biological significance of flavonoid cocoons of the silkworm, Bombyx mori

Research Project

Project/Area Number 23688008
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Applied entomology
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

DAIMON Takaaki  独立行政法人農業生物資源研究所, 昆虫成長制御研究ユニット, 主任研究員 (70451846)

Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywordsカイコ / フラボノイド / 遺伝子 / ゲノム / 生態学 / ゲノム編集 / RAD-seq / フェロモン / 繭 / TALEN / ノックアウト / 進化 / 遺伝学 / ポジショナルクローニング
Outline of Final Research Achievements

The purpose of this study is to reveal the molecular basis of flavonoid cocoons and their biological significances in the silkworm and its relative species. I performed a ddRAD-seq analysis in the silkworm and found that a novel locus that is involved in the flavonoid cocoon trait is located on the chromosome 20. I also revealed biological and ecological features of several species relative to the domesticated silkworm, and a great diversity in the flavonoid compounds contained in their cocoons. In addition, I established a highly efficient system for targeted mutagenesis in the silkworm, which can be a powerful tool for studying gene functions in a more sophisticated way.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (31 results)

All 2015 2014 2013 2012 Other

All Journal Article (14 results) (of which Peer Reviewed: 13 results,  Acknowledgement Compliant: 3 results,  Open Access: 2 results) Presentation (15 results) (of which Invited: 3 results) Book (2 results)

  • [Journal Article] The silkworm glutathione S-transferase gene noppera-bo is required for ecdysteroid biosynthesis and larval development2015

    • Author(s)
      Sora Enya, Takaaki Daimon, Fumihiko Igarashi, Hiroshi Kataoka, Miwa Uchibori, Hideki Sezutsu, Tetsuro Shinoda & Ryusuke Niwa.
    • Journal Title

      Insect Biochemistry and Molecular Biology

      Volume: 61 Pages: 1-7

    • DOI

      10.1016/j.ibmb.2015.04.001

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas92014

    • Author(s)
      S. Nakade, T. Tsubota, Y. Sakane, S. Kume, N. Sakamoto, M. Obara, T. Daimon, H. Sezutsu, T. Yamamoto, T. Sakuma and K. T. Suzuki
    • Journal Title

      Nat Commun

      Volume: 5 Issue: 1 Pages: 5560-5560

    • DOI

      10.1038/ncomms6560

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The transcription factor apontic-like controls diverse coloration pattern in caterpillars.2014

    • Author(s)
      Yoda S., Yamaguchi J., Mita K., Yamamoto K., Banno Y., Ando T., Daimon T. and Fujiwara H.
    • Journal Title

      Nat. Commun.

      Volume: 5 Issue: 1 Pages: 4936-4936

    • DOI

      10.1038/ncomms5936

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Antennal lobe organization and pheromone usage in bombycid moths2014

    • Author(s)
      Shigehiro Namiki, Takaaki Daimon, Chika Iwatsuki, Toru Shimada, Ryohei Kanzaki
    • Journal Title

      Biology Letters

      Volume: 10 Issue: 4 Pages: 20140096-20140096

    • DOI

      10.1098/rsbl.2014.0096

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] カイコガ亜科のバイオロジーと性フェロモン2014

    • Author(s)
      大門高明
    • Journal Title

      蚕糸・昆虫バイオテック

      Volume: 83 Pages: 105-114

    • NAID

      130004705721

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Anatomical and functional analysis of domestication effects on the olfactory system of the silkmoth Bombyx mori.2014

    • Author(s)
      Sonja Bisch-Knaden, Takaaki Daimon, Toru Shimada, Bill S. Hansson, and Silke Sachse
    • Journal Title

      Proceedings of the Royal Society B: Biological Sciences

      Volume: 281(1774) Issue: 1774 Pages: 20132582-20132582

    • DOI

      10.1098/rspb.2013.2582

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recent progress in genome engineering techniques in the silkworm, Bombyx mori.2014

    • Author(s)
      Takaaki Daimon, Takashi Kiuchi, and Yoko Takasu
    • Journal Title

      Develpment, Growth, and Differentiation

      Volume: 56 Issue: 1 Pages: 14-25

    • DOI

      10.1111/dgd.12096

    • NAID

      40019949214

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient TALEN construction for Bombyx gene targeting.2013

    • Author(s)
      Takasu Y, Sajwan S, Daimon T, Osanai-Futahashi M, et al(他4名)
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 9 Pages: e73458-e73458

    • DOI

      10.1371/journal.pone.0073458

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Flavonoids from the cocoon of Rondotia menciana2013

    • Author(s)
      Chikara Hirayama, Hiroshi Ono, Yan Meng, Toru Shimada, and Takaaki Daimon
    • Journal Title

      Phytochemistry

      Volume: 94 Pages: 108-112

    • DOI

      10.1016/j.phytochem.2013.05.023

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Function, diversity, and application of insect juvenile hormone epoxidases (CYP15)2013

    • Author(s)
      Takaaki Daimon
    • Journal Title

      Biotechnology and Applied Biochemistry

      Volume: 60 Pages: 82-91

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reinvestigation of the sex pheromone of the wild silkmoth Bombyx mandarina: The effects of bombykal and bombykyl acetate2012

    • Author(s)
      Takaaki Daimon
    • Journal Title

      Journal of Chemical Ecology

      Volume: 38 Pages: 1031-1035

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Precocious metamorphosis in the juvenile hormone-deficient mutant of the silkworm, Bombyx mori2012

    • Author(s)
      Takaaki Daimon, Toshinori Kozaki, Ryusuke Niwa, Isao Kobayashi, Kenjiro Furuta, Toshiki Namiki, Keiro Uchino, Yutaka Banno, Susumu Katsuma, Toshiki Tamura, Kazuei Mita, Hideki Sezutsu, Masayoshi Nakayama, Kyo Itoyama, Toru Shimada, Tetsuro Shinoda
    • Journal Title

      PLoS Genetics

      Volume: 8

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Female sex pheromone and male behavioral responses of the bombycid moth Trilocha varians : comparison with those of the domesticated silkmoth Bombyx mori2012

    • Author(s)
      Takaaki Daimon, Takeshi Fujii, Masaya Yago, Yu-Feng Hsu, Yumiko Nakajima, Tsuguru Fujii, Susumu Katsuma, Yukio Ishikawa, Toru Shimada
    • Journal Title

      Naturwissenschaften

      Volume: 99 Pages: 207-215

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular phylogeny, laboratory rearing, and karyotype of the bombycid moth, Trilocha varians (Lepidoptera : Bombycidae : Bombycinae)

    • Author(s)
      Takaaki Daimon, Masaya Yago, Yu-Feng Hsu, Tsuguru Fujii, Yumiko Nakajima, Ryuhei Kokusho, Hiroaki Abe, Susumu Katsuma, Toru Shimada
    • Journal Title

      Journal of Insect Science

      Volume: (in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] PTTHノックアウトカイコにおけるエクジステロイド生合成能2015

    • Author(s)
      内堀美和, 大門高明, 粥川琢巳, 瀬筒秀樹, 篠田徹郎
    • Organizer
      第59回日本応用動物昆虫学会大会
    • Place of Presentation
      山形大学
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] PTTH is not essential for development, but regulates developmental timing in Bombyx mori2014

    • Author(s)
      Uchibori M, Daimon T, Sezutsu H, Shinoda T
    • Organizer
      10th International Conference on Juvenile Hormones
    • Place of Presentation
      つくば市
    • Year and Date
      2014-06-09 – 2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Importance of JH signaling arises with competence of larvae to metamorphose2014

    • Author(s)
      Smykal V, Daimon T, Kayukawa T, Takaki K, Shinoda T, Jindra M
    • Organizer
      10th International Conference on Juvenile Hormones
    • Place of Presentation
      つくば市
    • Year and Date
      2014-06-09 – 2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] JH signalling molecules as targets for developing new IGRs2014

    • Author(s)
      Shinoda T, Kayukawa T, Furuta K, Daimon T
    • Organizer
      10th International Conference on Juvenile Hormones
    • Place of Presentation
      つくば市
    • Year and Date
      2014-06-09 – 2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Knockout studies on JH receptors and JH biosynthetic genes in the silkworm, Bombyx mori2014

    • Author(s)
      Daimon T, Uchibori M, Nakao H, Sezutsu H, Shinoda T
    • Organizer
      10th International Conference on Juvenile Hormones
    • Place of Presentation
      つくば市
    • Year and Date
      2014-06-09 – 2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] カイコとその近縁種における性フェロモンシステムの進化2012

    • Author(s)
      大門高明、嶋田透
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      近畿大学奈良キャンパス
    • Year and Date
      2012-03-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] Precocious metamorphosis in the CYP15C1-null mutant of the silkworm, Bombyx mori2012

    • Author(s)
      Takaaki Daimon
    • Organizer
      11th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
    • Place of Presentation
      イタリア、トリノ
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] カイコのゲノム編集とJH研究への展開

    • Author(s)
      大門高明
    • Organizer
      第58回日本応用動物昆虫学会大会 小集会
    • Place of Presentation
      高知大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] ノックアウトカイコを用いたJHシグナリング の解析

    • Author(s)
      大門高明・中尾 肇・内堀美和・ 瀬筒秀樹・篠田徹郎
    • Organizer
      平成26 年度 蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      日本大学湘南キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Highly efficient targeted mutagenesis in the silkworm Bombyx mori using TALENs

    • Author(s)
      Takaaki DAIMON
    • Organizer
      International symposium in RNAi and Genome editing methods
    • Place of Presentation
      徳島大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] カイコにおけるゲノム編集と幼若ホルモン研究への展開

    • Author(s)
      大門高明
    • Organizer
      Interdisciplinary symposium on advanced biology and biotechnology 2013
    • Place of Presentation
      産総研つくば中央
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Molecular characterization of moltinism mutants of the silkworm, Bombyx mori

    • Author(s)
      Takaaki Daimon
    • Organizer
      XXIV International Congress of Entomology
    • Place of Presentation
      韓国、テグ
    • Related Report
      2012 Annual Research Report
  • [Presentation] TALENのススメ:ノックアウト時代の到来

    • Author(s)
      大門 高明
    • Organizer
      平成25年度蚕糸・昆虫機能利用学術講演会-日本蚕糸学会第83回大会
    • Place of Presentation
      茨城県つくば市
    • Related Report
      2012 Annual Research Report
  • [Presentation] テンオビシロカサンのバイオロジーと性フェロモン

    • Author(s)
      大門 高明
    • Organizer
      平成25年度蚕糸・昆虫機能利用学術講演会-日本蚕糸学会第83回大会
    • Place of Presentation
      茨城県つくば市
    • Related Report
      2012 Annual Research Report
  • [Presentation] ノックアウトカイコ作出によるJHシグナリングの分子機構の解析

    • Author(s)
      大門 高明
    • Organizer
      第57回日本応用動物昆虫学会大会
    • Place of Presentation
      神奈川県藤沢市
    • Related Report
      2012 Annual Research Report
  • [Book] Targeted genome editing using site-specific nucleases2015

    • Author(s)
      Takaaki Daimon
    • Publisher
      Springer, Japan
    • Related Report
      2014 Annual Research Report
  • [Book] 実験医学別冊 最強のステップUPシリーズ「今すぐ始めるゲノム編集」2014

    • Author(s)
      大門高明
    • Total Pages
      9
    • Publisher
      羊土社
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-15   Modified: 2019-07-29  

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