Start-up study of the structural biology of cellulose synthase complex
| Project/Area Number |
23688021
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Wood science
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| Research Institution | Kyoto University |
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Principal Investigator |
IMAI Tomoya 京都大学, 生存圏研究所, 准教授 (90509142)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2011: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
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| Keywords | セルロース / セルロース合成酵素 / 電子顕微鏡 / 単粒子解析 / 構造生物学 / 機能再構成 / c-di-GMP / セルロース合成酵素複合体 / CesA / 大腸菌発現系 |
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| Research Abstract |
Electron microscopy was used for the three-dimensional structural determination of cellulose synthase complex. Preliminary analysis showed a three-dimensional model with some features. Our model is too primitive to be analyzed in detail, but it was shown as a result that the first X-ray crystallographic structure of cellulose synthase, which was reported in 2013 by another group, fits well to our structural model. We thus believe that our strategy is available for the structural biology of cellulose synthase, which will be used for future studies.
We also conducted, for discussing the structural data, some functional analyses of cellulose synthase: kinetics of crude enzyme and functional reconstitution of recombinant cellulose synthase in E. coli. Especially the latter result allows the functional analysis to be done with recombinant protein. We designated this system as CESEC (CEllulose Synthesis in E. Coli), which will be a promising tool for studying cellulose synthase.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Extraction ofcellulose-synthesizing activity ofGluconacetobacter xylinus byalkylmaltoside2011
Author(s)
Hashimoto, A., Shimono, K., Horikawa, Y., Ichikawa, T., Wada, M., Imai, T., Sugiyama, J
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Journal Title
Carbohydrate Research
Volume: 346(17)
Issue: 17
Pages: 2760-2768
DOI
Related Report
Peer Reviewed
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[Presentation] Functional reconstitution of cellulose synthase in Escherichia coli2013
Author(s)
Imai, T., Sun, S., Horikawa, Y., Wada, M., Sugiyama, J.
Organizer
EPNOE2013
Place of Presentation
Nice, France
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