| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Cytotoxic T lymphocytes (CTLs) exert anti-tumor effects through recognition of tumor antigen-derived peptides bound to human leukocyte antigens (HLAs) on cell surfaces. To identify diverse CTL epitopes of tumor antigens, mass spectrometry-based approach are examined. To investigate CTL epitope panel through tumor-specific antigen presenting machinery, MCF10A cells were transduced mutated K-ras gene. As a result, the gene-modified MCF10A cells had constitutively active autophagosomes and produced autophagosome-dependent epitopes. These data suggest that the cells having tumor-specific antigen presenting machinery provide us information on various CTL epitopes processed through the machinery.
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