Synthetic Technique of Polymers using Enzyme-immobilized Microreactors

• Project/Area Number: 23710153
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Microdevices/Nanodevices
• Research Institution: Tokai University
• Principal Investigator: YAMAGUCHI Hiroshi 東海大学, 阿蘇教養教育センター, 講師 (00466236)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 酵素固定化 / マイクロリアクター / 高分子合成

Research Abstract

In this research, we developed laccase-immobilized microreactor by using cross-linking of complex of enzyme and poly-lysine. The immobilized laccase maintained it catalytic activity after used several times. We also applied this laccase-immobilized microreactor to develop the polymer synthesis.

Research Products

• [Journal Article] Enzyme-immobilized reactors for rapid and efficient sample preparation in MS-based proteomic studies (2013)
  • Author(s): H. Yamagucjhi, M. Miyazaki
  • Journal Title: Proteomics Volume: 13巻 Pages: 457-466
  • Peer Reviewed
• [Journal Article] Enzyme-immobilized reactors for rapid and efficient sample preparation in MS-based proteomic studies (2013)
  • Author(s): 山口 浩
  • Journal Title: Proteomics Volume: 13 Issue: 3-4 Pages: 457-468
  • DOI: 10.1002/pmic.201200272
  • Peer Reviewed
• [Journal Article] Protease-immobilized microreactor for rapid and site-specific affinity Tag cleavage (2012)
  • Author(s): Y. Asanomi, Y. Koga, H. Yamaguchi, M. Miyazaki, T. Nose, H. Kodama, and H. Maeda
  • Journal Title: Peptide Science 2011 Pages: 395-396
  • NAID: 10030204636
  • Peer Reviewed
• [Journal Article] Simple and rapid proteomic analysis by protease-immobilized microreactors (2012)
  • Author(s): H. Yamaguchi, M. Miyazaki, and H. Maeda
  • Journal Title: Integrative Proteomics (Leung E. ed) Pages: 93-110
  • Peer Reviewed
• [Journal Article] Protease-immobilized microreactor for rapid and site-specific affinity Tag cleavage (2012)
  • Author(s): 浅海裕也
  • Journal Title: Peptide Science Volume: 2011 Pages: 395-396
  • Peer Reviewed
• [Journal Article] Simple and rapid proteomic analysis by protease-immobilized microreactors (2012)
  • Author(s): H. Yamaguchi, M. Miyazaki, and H. Maeda
  • Journal Title: Integrative Proteomics Volume: 1 Pages: 93-110
  • Peer Reviewed
• [Journal Article] Enzyme-immobilized microfluidic process reactors (2011)
  • Author(s): Y. Asanomi, H. Yamaguchi, M. Miyazaki, and H. Maeda
  • Journal Title: Molecules Volume: 16巻 Pages: 6041-6059
  • Peer Reviewed
• [Journal Article] Poly-lysine supported cross-linked enzyme aggregates with efficient enzymatic activity and high operational stability (2011)
  • Author(s): H. Yamaguchi, M. Miyazaki, Y. Asanomi,and H. Maeda
  • Journal Title: Catal. Sci. Technol Volume: 1巻 Pages: 1256-1261
  • Peer Reviewed
• [Presentation] マイクロ化学デバイスを用いた効率的アフィニティータグ切断法の開発 (2012)
  • Author(s): 山口 浩ほか
  • Organizer: 平成24年度 日本生化学会九州支部例会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-05-26
• [Presentation] Laccase-immobilized microreactors for the removal of estrogenic compounds (2012)
  • Author(s): 山口 浩ほか
  • Organizer: 第25回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 熊本市
  • Year and Date: 2012-05-17
• [Presentation] マイクロ化学デバイスを用いた効果的アフィニティータグ切断法の開発 (2012)
  • Author(s): 浅海裕也
  • Organizer: 平成24年度 日本生化学会九州支部例会
  • Place of Presentation: 福岡大学
• [Presentation] Laccase-immobilized microreactors for the removal of estrogenic compounds (2012)
  • Author(s): L. Lloret
  • Organizer: 第25回 化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 崇城大学
• [Presentation] 酵素架橋凝集体の触媒能と安定性解析 (2011)
  • Author(s): 山口 浩ほか
  • Organizer: 第48回 化学関連支部合同九州大会
  • Place of Presentation: 北九州市
  • Year and Date: 2011-07-09
• [Presentation] 酵素架橋凝集体の触媒能と安定性解析 (2011)
  • Author(s): 山口 浩、浅海裕也、宮崎真佐也、前田英明
  • Organizer: 第48回化学関連支部合同九州大会
  • Place of Presentation: 北九州国際会議場