| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Alkyne-tag Raman imaging was proposed as an approach for live cell imaging of small molecules by using an alkyne-tag. Structure-Raman shift/intensity relationships of various alkynes was examined by use of EdU as standard. On the basis of obtained results, simultaneous imaging of two alkyne-tagged molecules, EdU and alkyne-tagged CoQ analogue (AltQ), and semiquantitative estimation of cellular concentration of CoQ analogues have been achieved. Finally, simultaneous imaging of protonated and deprotonated carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP) was successfully demonstrated.
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