Identifying the evolutionary significant units of Japanese Siluridae: towards implementation of genetic monitoring
| Project/Area Number |
23710288
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Resource conservation science
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| Research Institution | National Institute for Environmental Studies |
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Principal Investigator |
MATSUZAKI Shin-ichiro 独立行政法人国立環境研究所, 生物・生態系環境研究センター, 研究員 (40548773)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 淡水魚 / 進化的重要単位 / 地域固有性 / マイクロサテライトマーカー / 系統地理 / 遺伝的多様性 / 国内移入 / 放流 / ナマズ / 地域集団 / 遺伝的モニタリング / ミトコンドリアDNA / DNA / ESU |
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| Research Abstract |
To elucidate the genetic population structure of three native Siluridae in Japan, we collected specimens from Japan, Korea, China and Vietnam, and sequenced the mitochondrial DNA control region. Phylogenetic analyses revealed one, two and three distinct genetic lineages for Silurus biwaensis, Silurus lithophilus and Silurus asotus, respectively. Although Silurus lithophilus is well-known as one of the endemic species of Lake Biwa, we found that other lineages are naturally distributed in the Kinki and Chubu regions. We also demonstrated that Silurus asotus in eastern Japan may be translocated intentionally from the native regions such as Kinki and Chugoku regions. Toward a genetic monitoring, we developed microsatellite markers using next-generation sequencing.
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Report
(4 results)
Research Products
(16 results)
