Development of High Performance Enzyme Activity Assay for Glycoside Hydrolase Using Metal-ions as Indicator
| Project/Area Number |
23750075
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Fukushima University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 酵素活性測定 / バイオマス / セルロース / セルラーゼ / 分析化学 / 酵素分析 / ICP-MS / 原子吸光光度分析 / ICP / 国際研究者交流 |
|---|
| Research Abstract |
In this study, the simple determination method of cellulase has been developed using copper(II)-phthalocyanine complex modified cellulose with the alternative of an inductively coupled plasma -optical emission spectrometry (ICP-OES) or flame-less atomic absorbance spectrometer(FL-AAS). Then, although the cellulase does not include the metal ion in the molecule, the metal ion is released by the biodigestion of copper(II)-phthalocyanine complex modified cellulose into the solution. The presented method can measure the cellulase concentration and the activity in short time (within 15min) as comparing with common spectrophotometric method.
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Report
(4 results)
Research Products
(32 results)
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[Book] 阿武隈川流域の環境学2011
Author(s)
（分担著書）高貝慶隆, 福島大学自然共生・再生研究プロジェクト 柴崎直明,塘忠顕, 長橋良隆, 渡邊明 編著
Total Pages
273
Publisher
福島民報社
Related Report
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