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Mechanism of structural maintenance of mammalian rDNA cluster

Research Project

Project/Area Number 23770009
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Genetics/Genome dynamics
Research InstitutionNational Institute of Genetics

Principal Investigator

AKAMATSU Yufuko  国立遺伝学研究所, 細胞遺伝研究系, 助教 (50381661)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsrDNA / DNA複製 / 複製フォーク進行阻害 / ゲノム再編成 / リボソームRNA遺伝子 / DNA複製阻害
Research Abstract

To understand molecular mechanism maintaining mammalian rDNA cluster, DNA replication fork barrier (RFB) at 3' end of rRNA coding region was investigated. Using two -dimensional gel electrophoresis method, DNA replication fork movement was visualized. In mammalian cells, multiple RFB signals were detected and a part of them, as previously reported, were TTF1 dependent to block movement of replication fork. The other RFB, however, did not involve TTF-1, rather previously unknown mechanism functions. These results imply that collision between replication fork and rRNA transcription is prevented through multiple pathways in mammalian rDNA cluster.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (3 results)

All 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] Rad51 presynaptic filament stabilization function of the mouse Swi5-Sfr1 heterodimeric complex.2012

    • Author(s)
      Shang-Pu Tsai
    • Journal Title

      Nucleic Acids Research

      Volume: 40 Issue: 14 Pages: 6558-6569

    • DOI

      10.1093/nar/gks305

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Characterization of the mammalian rDNA replication fork barrier2012

    • Author(s)
      Yufuko Akamatsu
    • Organizer
      The 8th 3R Symposium
    • Place of Presentation
      淡路夢舞台国際会議場
    • Year and Date
      2012-11-25
    • Related Report
      2012 Final Research Report
  • [Presentation] Characterization of the mammalian rDNA replication fork barrier2012

    • Author(s)
      Yufuko Akamatsu
    • Organizer
      The 8th 3R Symposium
    • Place of Presentation
      Awaji Yumebutai International Conference Center, Japan
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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