Specification of sensory primordia by the synergistic action of SOX2 and its partner factor

• Project/Area Number: 23770248
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Osaka University
• Principal Investigator: UCHIKAWA Masanori 大阪大学, 生命機能研究科, 助教 (80346147)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 細胞分化 / 発現制御 / 感覚器原基 / エンハンサー / Sox2 / Sall4 / SOX蛋白質 / Sall4蛋白質 / 感覚器形成 / 嗅上皮プラコード / 内耳プラコード / 転写制御因子 / プラコード / 嗅上皮 / 内耳

Research Abstract

Sensory tissues, e.g. olfactory epithelium, inner ear and lens, develop from cephalic ectoderm through progressive specification steps. The transcription factor gene Sox2 is consistently expressed in the sensory primordia regardless of developmental stages, owing to the sequential activation of stage-/placode-specific enhancers. The NOP1 enhancer is specifically active in the nasal and otic placodes. An essential activating element of NOP1 enhancer was shown to be regulated by the synergistic action of Sox2 and Sall4, as well as Sox9 and Sall4 (but not Sox11 and Sall4). Inhibition of Sox activity in the posterior cephalic ectoderm inactivated the NOP1 enhancer. These data indicate that Sox-Sall4 synergism is essential for Sox2regulation in the nasal and otic placodes.

Research Products

• [Journal Article] Progression of neurogenesis in the inner ear requires inhibition of Sox2 transcription by neurogenin1 and neurod1 (2013)
  • Author(s): Evsen, L., Sugahara, S., Uchikawa, M., Kondoh, H., Wu, DK.
  • Journal Title: J Neurosci Volume: 33 Issue: 9 Pages: 879-890
  • DOI: 10.1523/jneurosci.4030-12.2013
  • Peer Reviewed
• [Journal Article] A systematic survey and characterization of enhancers that regulate Sox3 in neurosensory development in comparison with Sox2 enhancers (2012)
  • Author(s): Nishimura, N., Kamimura, Y., Ishida, Y., Takemoto, T., Kondoh, H., Uchikawa, M.
  • Journal Title: Biology Volume: 1 Issue: 3 Pages: 714-735
  • DOI: 10.3390/biology1030714
  • Peer Reviewed
• [Journal Article] The Prosensory Function of Sox2 in the Chicken Inner Ear Relies on the Direct Regulation of Atoh1. (2012)
  • Author(s): Joana Neves, Masanori Uchikawa, Anna Bigas, Fernando Giraldez
  • Journal Title: PLoS ONE Volume: 7(1) Issue: 1 Pages: e30871-e30871
  • DOI: 10.1371/journal.pone.0030871
  • Peer Reviewed
• [Journal Article] The Pou5f1/Pou3f-dependent but SoxB-independent regulation of conserved enhancer N2 initiates Sox2 expression during epiblast to neural plate stages in vertebrates (2011)
  • Author(s): Iwafuchi-Doi M
  • Journal Title: Developmental Biology Volume: 352 Issue: 2 Pages: 354-366
  • DOI: 10.1016/j.ydbio.2010.12.027
  • Peer Reviewed
• [Journal Article] B1 and B2 Sox gene expression during neural plate development in chicken and mouse embryos : Universal versus species-dependent features (2011)
  • Author(s): Uchikawa M
  • Journal Title: Development, Growth, and Differentiation Volume: 53 Issue: 6 Pages: 761-771
  • DOI: 10.1111/j.1440-169x.2011.01286.x
  • Peer Reviewed
• [Presentation] GroupB1/E Sox 因子と Sall4 の協調的な活性化と多数の抑制エレメントによるSox2 遺伝子の鼻・耳プラコード特異的NOP1 エンハンサーの発現制御 (2012)
  • Author(s): 内川 昌則,須賀原 智子,近藤 寿人
  • Organizer: 第35回日本分子生物学会年会 3P-0556
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
  • Year and Date: 2012-12-13
• [Presentation] 初期ニワトリ胚を用いたzfhx1 ファミリーの機能重複の検討 (2012)
  • Author(s): 安見 孝広, 内川 昌則, 近藤 寿人, 東 雄二郎
  • Organizer: 第35回日本分子生物学会年会 3P-0504
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
  • Year and Date: 2012-12-13
• [Presentation] 内耳原基で活性を示すニワトリ Sox3 遺伝子のエンハンサーの解析 (2012)
  • Author(s): 岡本 優, 西村 なおこ, 内川 昌則, 近藤 寿人
  • Organizer: 第35回日本分子生物学会年会 2P-0528
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
  • Year and Date: 2012-12-12
• [Presentation] Regulation of Sox2 NOP1 enhancer indicates Sox-Sall4 dependent activation and multiple repression mechanisms involved in the nasal and otic placode specification (2012)
  • Author(s): Masanori Uchikawa, Satoko Sugahara, Hisato Kondoh
  • Organizer: Chick 7: Avian Model System 7th International Chick Meeting P1-22
  • Place of Presentation: Nagoya University Noyori Conference Hall, Nagoya, Japan
• [Presentation] Small eye development caused by ectodermal Sox2 downregulation in enhancer N4 knockout mice (2011)
  • Author(s): Masanori Uchikawa, Miho Morishima, Yuka Saigou, Hisato Kondoh
  • Organizer: 日本発生生物学会 第44回大会 P-2086
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2011-05-20
• [Presentation] Nasal/otic placode-specific regulation of Sox2 enhancer NOP-1 depends on SoxB1/E-Sall4 interaction and multiple repression mechanisms (2011)
  • Author(s): Satoko Sugahara, Toru Fujimoto, Masanori Uchikawa, Hisato Kondoh
  • Organizer: Third International Sox Meeting: From Structure To Function
  • Place of Presentation: Grainau/Zugspitzdorf, Germany
• [Presentation] Small eye development caused by ectodermal Sox2 downregulation in enhancer N4 knockout mice (Sox2遺伝子エンハンサーN4ノックアウトマウスにおける小眼発生の機構) (2011)
  • Author(s): Masanori Uchikawa, Miho Morishima, Yuka Saigou, Hisato Kondoh
  • Organizer: 日本発生生物学会 第44回大会
  • Place of Presentation: 沖縄コンベンションセンター
• [Presentation] Nasal/otic placode-specific regulation of Sox2 enhancer NOP-1 depends on SoxB1/E-Sall4 interaction and multiple repression mechanisms (2011)
  • Author(s): Masanori Uchikawa, Satoko Sugahara, Toru Fujimoto, Hisato Kondoh
  • Organizer: Third International Sox Meeting
  • Place of Presentation: Grainau/Zugspitzdorf(ドイツ)
• [Presentation] Regulation of Sox2 NOP1 enhancer indicates Sox-Sall4 dependent activation and multiple repression mechanisms involved in the nasal and otic placode specification
  • Author(s): Masanori Uchikawa
  • Organizer: 7th International Chick Meeting
  • Place of Presentation: Nagoya University Noyori Conference Hall, Nagoya, Japan
• [Presentation] GroupB1/E Sox 因子とSall4 の協調的な活性化と多数の抑制エレメントによるSox2 遺伝子の鼻・耳プラコード特異的NOP1 エンハンサーの発現制御
  • Author(s): 内川 昌則
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Presentation] 内耳原基で活性を示すニワトリSox3 遺伝子のエンハンサーの解析
  • Author(s): 岡本 優
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Presentation] 初期ニワトリ胚を用いたzfhx1 ファミリーの機能重複の検討
  • Author(s): 安見 孝広
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡