Study of the molecular basis on evolution of plant development explored in a unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex

• Project/Area Number: 23770277
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Evolutionary biology
• Research Institution: Chuo University (2012-2014); Japan Women's University (2011)
• Principal Investigator: ABE Jun 中央大学, 研究開発機構, 機構助教 (10424764)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ヒメミカヅキモ / シャジクモ藻類 / 転写因子 / 発生進化 / 有性生殖 / 性決定 / 多細胞化 / ゲノム / Major Intrinsic Protein

Outline of Final Research Achievements

Charophycean green algae are the closest living relatives to land plants. To understand how plant transcription factors (TFs) diversified in Charophycean algae, we investigated genome in a unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex. We found that 75% of "land plant-specific" TF families that not found in Chlorophycean algae present in Closterium genome. We also found a Closterium TF, named CpMinus1, only present in mating-type minus genome. Transformation analysis indicated that the CpMinus1 is responsible for the sex determination in Closterium.

Research Products

• [Journal Article] The evolution of male-female sexual dimorphism predates the gender-based divergence of the mating locus gene MAT3/RB. (2013)
  • Author(s): Rintaro Hiraide
  • Journal Title: Molecular Biology and Evolution Volume: 30 Issue: 5 Pages: 1038-1040
  • DOI: 10.1093/molbev/mst018
  • Peer Reviewed
• [Journal Article] Properties of cell surface carbohydrates in sexual reproduction of the Closterium peracerosum-strigosum-littorale complex (Zygnematophyceae, Charophyta) (2012)
  • Author(s): Hori, S., Sekimoto, H., Abe, J
  • Journal Title: Phycol. Res Volume: 60 Issue: 4 Pages: 254-260
  • DOI: 10.1111/j.1440-1835.2012.00656.x
  • NAID: 10031136641
  • Peer Reviewed
• [Journal Article] StableNuclear Transformation of Closteriumperacerosum-strigosum-littoralecomplex. (2011)
  • Author(s): Abe, J., Hori, S., Tsuchikane, Y., Kitao, N.,Kato, M. & Sekimoto, H.
  • Journal Title: Plant Cell Physiol. Volume: 52 Issue: 9 Pages: 1676-1685
  • DOI: 10.1093/pcp/pcr103
  • Peer Reviewed
• [Journal Article] Conjugationprocesses of Penium margaritaceum(Zygnemophyceae, Charophyta) (2011)
  • Author(s): Tsuchikane, Y., Tsuchiya, M., Kokubun, Y.,Abe , J. & Sekimoto, H.
  • Journal Title: Phycol.Res Volume: 59 Issue: 1 Pages: 74-82
  • DOI: 10.1111/j.1440-1835.2010.00602.x
  • Peer Reviewed
• [Presentation] 油脂生産性緑藻Pseudococcomyxa sp. KJ株における形質転換と導入遺伝子発現系の開発 (2015)
  • Author(s): 阿部淳、井出曜子、高木さつき、今村壮輔、原山重明
  • Organizer: 第9回日本ゲノム微生物学会
  • Place of Presentation: 神戸大学（神戸）
  • Year and Date: 2015-03-06 – 2015-03-08
• [Presentation] ヒメミカヅキモの性フェロモンと結合するファシクリンIタンパク質の機能解析 (2014)
  • Author(s): 市原健介、滝口若菜、原真由美、土金勇樹、阿部淳、関本弘之
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学（川崎）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] ヒメミカヅキモの-型細胞ゲノム特異的遺伝子 CpMinus1 の機能解析 (2014)
  • Author(s): 小宮あゆみ、阿部淳、川井絢子、鈴木穣、豊田敦、藤山秋佐夫、大槻涼、土金勇樹、西山智明、関本弘之
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学（川崎）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] シャジクモ藻類ヒメミカヅキモにおける陸上植物特異的な転写因子の探索と発現解析 (2014)
  • Author(s): 川井絢子、阿部淳、鈴木穣、豊田敦、藤山秋佐夫、土金勇樹、西山智明、関本弘之
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学（川崎）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] ヒメミカヅキモの－型細胞特異的遺伝子CpMinus1の解析 (2014)
  • Author(s): 小宮あゆみ、阿部淳、川井絢子、鈴木穣、豊田敦、藤山秋佐夫、大槻涼、土金勇樹、西山智明、関本弘之
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山市 富山大学
• [Presentation] オイル生産藻類Pseudococcomyxa ellipsoideaにおける安定した導入遺伝子発現系の開発 (2013)
  • Author(s): 阿部 淳、吉満 勇也、高木 さつき、福原 いずみ、藏野 憲秀、今村 壮輔、原山 重明
  • Organizer: 第7回日本ゲノム微生物学会
  • Place of Presentation: 長浜バイオ大学（長浜）
• [Presentation] 藻類の遺伝子操作の現状と課題 ―単細胞シャジクモ藻類ヒメミカヅキモを例として― (2012)
  • Author(s): 阿部淳、関本弘之
  • Organizer: 日本植物整理学会第53回大会(招待講演)
  • Place of Presentation: 京都産業大学 （京都）
• [Presentation] Closterium and Chara genome and transcriptome analyses (2012)
  • Author(s): Jun Abe, Hidetoshi Sakayama, Yutaka Suzuki, Atsushi Toyoda, Yuki Tsuchikane, Hiroyuki Sekimoto, Tomoaki Nishiyama
  • Organizer: Plant and Animal Genome XX Conference
  • Place of Presentation: San Diego, USA
• [Presentation] 単細胞シャジクモ藻ヒメミカヅキモのゲノミクス (2011)
  • Author(s): 阿部淳、関本弘之
  • Organizer: 日本植物学会第75回大会(招待講演)
  • Place of Presentation: 東京大学（駒場）
• [Presentation] Identification and phylogenetic analyses of aquaporins from the unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex (2011)
  • Author(s): Jun Abe, Kaoru Taki, Tomoaki Nishiyama, Yuki Tsuchikane, Hiroyuki Sekimoto
  • Organizer: 6th Asian Pasific Phycological Forum
  • Place of Presentation: Yeosu, Korea
• [Presentation] Stable transformation system for a unicellular charophycean alga, Closterium-peracerosum-strigosum-littorale complex (2011)
  • Author(s): Jun Abe, Sachie Hori, Hiroyuki Sekimoto
  • Organizer: 6th Asian Pasific Phycological Forum
  • Place of Presentation: Yeosu, Korea
• [Presentation] Effect of lectin on the sexual reproduction of Closterium peracerosum-strigosum-littorale complex (2011)
  • Author(s): Sachie Hori, Jun Abe, Mamiko Sato and Hiroyuki Sekimoto
  • Organizer: 6th Asian Pasific Phycological Forum
  • Place of Presentation: Yeosu, Korea
• [Presentation] Characterization of a novel receptor-like protein kinase, CpRLK1, involved in the sexual reproduction of a unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex (2011)
  • Author(s): Naoko Hirano, Yuka Marukawa, Saori Hashiba, Yuki Tsuchikane, Jun Abe, Hiroyuki Sekimoto
  • Organizer: 6th Asian Pasific Phycological Forum
  • Place of Presentation: Yeosu, Korea
• [Presentation] Construction of stable transformation system for a unicellular charophycean alga, Closterium-peracerosum-strigosum-littorale complex (2011)
  • Author(s): Jun Abe, Sachie Hori, Hiroyuki Sekimoto
  • Organizer: International Symposium on Algal Biofuels
  • Place of Presentation: Tokyo, Japan
• [Presentation] 単細胞シャジクモ藻ヒメミカヅキモから見た植物アクアポリンファミリーの進化 (2011)
  • Author(s): 阿部淳、瀧薫、西山智明、土金勇樹、関本弘之
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学（駒場）
• [Presentation] ヒメミカヅキモの接合過程に関与するCon A標的分子の局在と機能 (2011)
  • Author(s): 堀早知恵、阿部淳、佐藤眞美子、関本弘之
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学（駒場）
• [Book] International review of cell and molecular biology, Jeon, K.W. ed. Academic Press (2012)
  • Author(s): Sekimoto H, Abe J, Tsuchikane Y
  • Publisher: New insights into the regulation of sexual reproduction in Closterium