Characterization of the reversion mechanisms of polyphosphate-accumulating mutants
| Project/Area Number |
23780083
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Hiroshima University |
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Principal Investigator |
HIROTA Ryuichi 広島大学, 大学院・先端物質科学研究科, 助教 (90452614)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | ポリリン酸 / PhoU / リン酸レギュロン / phoU / 変異 |
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| Research Abstract |
We previously revealed that a mutation inPhoU, a negative regulator of bacterial phosphate regulon, results in the elevated levels of polyphosphate (polyP) in cell. However, PhoU mutants were rapidly outgrownby revertant(s) that lost the ability to accumulate polyP. In this study, we analyzed the mechanisms that the revertants lost polyP-accumulating ability, and established a method to screen stable PhoU mutants. The discovery in this study would be applicable to the development of a phosphorus-recovering technology.
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Report
(3 results)
Research Products
(31 results)
