| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Research Abstract |
In present study, I investigated the osteoclast inhibition ability in CS and the mechanism how CS inhibit osteoclast differentiation in vitro. CS was significantly inhibited the number of tartrate-resistance acid phosphatase (TRAP)-positive multinucleated cells and bone resorption activity in pit formation assay.In quartz-crystal microbalance method, CS revealed the binding ability to RANKL and interrupted binding of RANK to RANKL. Furthermore, CS reduced the phosphorylation of extracellular ERK in pre-osteoclast cells. These results indicate that CS suppress osteoclast size and osteoclastogenesis by binding to RANKL and inhibited RANK signal pathway.
|
