Analysis of Ebola virus nucleocapsid formation

• Project/Area Number: 23780309
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied veterinary science
• Research Institution: The University of Tokyo
• Principal Investigator: NODA Takeshi 東京大学, 医科学研究所, 准教授 (00422410)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: エボラウイルス / ヌクレオカプシド / ウイルス / 電子顕微鏡

Research Abstract

Ebola virus VP35 is a cofactor of the viral RNA polymerase complex and, together with NP and VP24, is an essential component for nucleocapsid formation. In the present study, we examined the interactions between VP35 and NP and found that VP35 interacts with helical NP-RNA complexes through the C-terminus of NP. We also found that coexpression of excess VP35 with NP reduced the yields of NP-RNA complexes purified by CsCl gradient ultracentrifugation and inhibited the formation of the NP-induced inclusion bodies that typically form in Ebola virus-infected cells. These findings suggest that the NP to VP35 ratio is important in the Ebola virus replication cycleand advance our knowledge of nucleocapsid morphogenesis.

Research Products

• [Journal Article] Identification of novel influenza A virus proteins translated from PA mRNA. (2013)
  • Author(s): Muramoto Y
  • Journal Title: J Virol Volume: 87 Issue: 5 Pages: 2455-62
  • DOI: 10.1128/jvi.02656-12
  • Peer Reviewed
• [Journal Article] The microtubule motor protein KIF13A is involved in intracellular trafficking of the Lassa virus matrix protein Z. (2013)
  • Author(s): Fehling SK
  • Journal Title: Cell Microbiol Volume: 15 Issue: 2 Pages: 315-34
  • DOI: 10.1111/cmi.12095
  • Peer Reviewed
• [Journal Article] Structural dissection of Ebola virus and its assembly determinants using cryo-electron tomography (2012)
  • Author(s): Bharat T, Noda T, Riches JD, Krahling V, Kolesnikova L, Becker S, Kawaoka Y, Briggs J
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 109 Pages: 4275-80
  • Peer Reviewed
• [Journal Article] Inhibition of Marburg virus budding by nonneutralizing antibodies to the envelope glycoprotein (2012)
  • Author(s): Kajihara M, Marzi A, Nakayama E, Noda T, Kuroda M, Manzoor R, Matsuno K, Feldmann H, Yoshida R, Kawaoka Y, Takada A
  • Journal Title: J. Virol Volume: 86 Pages: 13467-74
  • NAID: 120005228739
  • Peer Reviewed
• [Journal Article] Packaging of influenza virus genome: robustness of selection. (2012)
  • Author(s): Noda T
  • Journal Title: Proc Natl Acad Sci U S A Volume: 109 Issue: 23 Pages: 8797-8
  • DOI: 10.1073/pnas.1206736109
  • Peer Reviewed
• [Journal Article] Inhibition of Marburg virus budding by nonneutralizing antibodies to the envelope glycoprotein. (2012)
  • Author(s): Kajihara M, Marzi A, Nakayama E, Noda T, Kuroda M, Manzoor R, Matsuno K, Feldmann H, Yoshida R, Kawaoka Y, Takada A.
  • Journal Title: J Virol. Volume: 86 Issue: 24 Pages: 13467-13474
  • DOI: 10.1128/jvi.01896-12
  • NAID: 120005228739
  • Peer Reviewed
• [Journal Article] A large extension to HIV-1 Gag, like Pol, has negative impacts on virion assembly. (2012)
  • Author(s): Haraguchi H
  • Journal Title: PLoS One Volume: 7 Issue: 10 Pages: e47828-e47828
  • DOI: 10.1371/journal.pone.0047828
  • Peer Reviewed
• [Journal Article] Structural dissection of Ebola virus and its assembly determinants using cryo-electron tomography. (2012)
  • Author(s): Bharat TA, Noda T, Riches JD, Kraehling V, Kolesnikova L, Becker S, Kawaoka Y, Briggs JA.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 109 Pages: 4275-80
  • Peer Reviewed
• [Journal Article] The importance of the NP:VP35 ratio in Ebola virus nucleocapsid formation (2011)
  • Author(s): Noda T, Kolesnikova L, Becker S, Kawaoka Y
  • Journal Title: J. Infect. Dis Volume: 3
  • Peer Reviewed
• [Journal Article] Identification of amino acids in Marburg virus VP40 that are important for virus-like particle budding (2011)
  • Author(s): Makino A, Yamayoshi S, Shinya K, Noda T, Kawaoka Y
  • Journal Title: J. Infect. Dis Volume: 3
  • Peer Reviewed
• [Journal Article] Contribution of Sec61a to the life cycle of Ebola virus (2011)
  • Author(s): Iwasa A, Halfmann P, Noda T, Oyama M, Kozuka-Hata H, Watanabe S, Shimojima M, Watanabe T, Kawaoka Y
  • Journal Title: J. Infect. Dis. Suppl Volume: 3
  • Peer Reviewed
• [Journal Article] The importance of the NP: VP35 ratio in Ebola virus nucleocapsid formation. (2011)
  • Author(s): Noda T, Kolesnikova L, Becker S, Kawaoka Y.
  • Journal Title: J Infect Dis. Volume: 204 Suppl 3
  • Peer Reviewed
• [Journal Article] Identification of amino acids in Marburg virus VP40 that are important for virus-like particle budding. (2011)
  • Author(s): Makino A, Yamayoshi S, Shinya K, Noda T, Kawaoka Y.
  • Journal Title: J Infect Dis. Volume: 204 Suppl 3
  • Peer Reviewed
• [Journal Article] Contribution of Sec61α to the life cycle of Ebola virus. (2011)
  • Author(s): Iwasa A, Halfmann P, Noda T, Oyama M, Kozuka-Hata H, Watanabe S, Shimojima M, Watanabe T, Kawaoka Y.
  • Journal Title: J Infect Dis. Volume: 204 Suppl 3 Pages: 919-26
  • Peer Reviewed
• [Presentation] Three-dimensional analysis of ribonucleoprotein complexes in influneza A virus particles (2012)
  • Author(s): Takeshi Noda
  • Organizer: Joint Seminar CBIS-Structural biology division of Japan EM
  • Place of Presentation: Singapore
• [Presentation] インフルエンザウイルスの細胞内増殖機構
  • Author(s): 野田岳志
  • Organizer: 平成24年度遺伝子病制御研究所研究集会 感染・免疫・炎症・発癌
  • Place of Presentation: 札幌
  • Invited
• [Presentation] Three dimensional analysis of ribonucleoprotein complexes in influenza A virus particles
  • Author(s): Takeshi Noda
  • Organizer: 31st American Society for Virology Meeting
  • Place of Presentation: Madison, WI, USA
• [Presentation] A型インフルエンザウイルスPA遺伝子由来新規ウイルス蛋白質の探索
  • Author(s): 野田岳志
  • Organizer: 第22回日本数理生物学会
  • Place of Presentation: 岡山
• [Presentation] インフルエンザウイルスのゲノムパッケージング機構の解析
  • Author(s): 野田岳志
  • Organizer: 第61回日本感染症学会東日本地方学術集会
  • Place of Presentation: 東京
  • Invited
• [Presentation] インフルエンザウイルスのゲノムパッケージング機構
  • Author(s): 野田岳志
  • Organizer: 第61回日本分子生物学会
  • Place of Presentation: 福岡
  • Invited