Analysis of polyamine regulation systems in plants and exploration of functional molecules based on polyamine structure

• Project/Area Number: 23780345
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Tohoku University
• Principal Investigator: TAKAHASHI Yoshihiro 東北大学, 大学院・生命科学研究科, 准教授 (20390891)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 代謝工学 / ポリアミン / ポリアミンオキシターゼ / イネ / 病原菌抵抗性反応 / シロイヌナズナ

Research Abstract

Polyamines are important not only for developmental processes but also for various environmental stress responses in plants. In order to elucidate the regulatory system responsible for polyamine homeostasis and action, I focused on polyamine degradationenzyme, polyamine oxidase (PAO), and identified three different genes coding for main PAOs in rice. These proteins were localized to peroxisomes and catalyzed polyamine back conversion. Moreover, it was found that one of the common tetraamines, thermospermine, is involved in disease resistance as a signal molecule like spermine.

Research Products

• [Journal Article] Exogenous thermospermine has an activity to induce a subset of the defense genes and restrict cucumber mosaic virus multiplication in Arabidopsis thaliana. (2012)
  • Author(s): Sagor GHM, Takahashi H, Niitsu M, Takahashi Y, Berberich T, Kusano T
  • Journal Title: Plant Cell Reports Volume: (In press) Issue: 7 Pages: 1227-1232
  • DOI: 10.1007/s00299-012-1243-y
  • Peer Reviewed
• [Journal Article] Constitutively and highly expressed Oryza sativa polyamine oxidases localize in peroxisomes and catalyze polyamine back conversion (2012)
  • Author(s): Ono, Y., Kim, D.W., Watanabe, K., Sasaki, A., Niitsu, M., Berberich, T., Kusano, T. and Takahashi, Y
  • Journal Title: Amino Acids Volume: Vol.42 Issue: 2-3 Pages: 867-876
  • DOI: 10.1007/s00726-011-1002-3
  • Peer Reviewed
• [Journal Article] Constitutively and highly expressed Oryza sativa polyamine oxidases localize in peroxisomes and catalyze polyamine back conversion (2012)
  • Author(s): Ono, Y., Kim, D.W., Watanabe, K., Sasaki, A., Niitsu, M., Berberich, T., Kusano, T. and Takahashi, Y.
  • Journal Title: Amino Acids Volume: 42 Pages: 867-876
  • Peer Reviewed
• [Journal Article] Spatio-temporal expression analysis of Arabidopsis thaliana spermine synthase gene promoter (2011)
  • Author(s): Sagor, G.H.M., Yamaguchi, K., Watanabe, K., Berberich, T., Kusano, T. and Takahashi, Y
  • Journal Title: Plant Biotechnology Volume: 28 Issue: 4 Pages: 407-411
  • DOI: 10.5511/plantbiotechnology.11.0704a
  • NAID: 10029707427
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] Spatio-temporal expression analysis of Arabidopsis thaliana spermine synthase gene promoter (2011)
  • Author(s): Sagor, G.H.M., Yamaguchi, K., Watanabe, K., Berberich, T., Kusano, T. and Takahashi, Y.
  • Journal Title: Plant Biotechnology Volume: 28 Pages: 407-411
  • NAID: 10029707427
  • Peer Reviewed
• [Presentation] スペルミンは熱ショック関連遺伝子群の発現を高めることによりシロイヌナズナを熱ショック傷害から保護する (2013)
  • Author(s): Sagor GHM,Berberich Thomas,高橋芳弘,新津勝,草野友延
  • Organizer: 日本ポリアミン学会第4回年会
  • Place of Presentation: 宮城
  • Year and Date: 2013-01-25
• [Presentation] T-DNA挿入pao変異体植物の外部投与ポリアミンへの反応性に基づくシロイヌナズナのポリアミン酸化酵素の基質特異性への考察 (2012)
  • Author(s): 土橋隼人,Sagor GHM,新津勝,高橋芳弘,Thomas Berberich,草野友延
  • Organizer: 日本ポリアミン学会第3回年会
  • Place of Presentation: 埼玉
  • Year and Date: 2012-01-26
• [Presentation] T-DNA挿入pao変異体植物の外部投与ポリアミンへの反応性に基づくシロイヌナズナのポリアミン酸化酵素の基質特異性への考察 (2012)
  • Author(s): 土橋隼人，Sagor GHM， 新津 勝，高橋芳弘，Thomas Berberich，草野友延
  • Organizer: 日本ポリアミン学会
  • Place of Presentation: 埼玉
• [Presentation] イネにおけるポリアミン酸化酵素の特徴づけ (2011)
  • Author(s): 金東〓,小野裕介,渡辺佳奈子,佐々木彩乃,新津勝,Berberich Thomas,草野友延,高橋芳弘
  • Organizer: 第146回日本農芸化学会東北支部会
  • Place of Presentation: 山形
  • Year and Date: 2011-10-08
• [Presentation] イネにおけるポリアミン酸化酵素の特徴づけ (2011)
  • Author(s): 金東煜，小野裕介，渡辺佳奈子，佐々木彩乃，新津 勝，Berberich Thomas，草野友延，高橋芳弘
  • Organizer: 日本農芸化学会東北支部会
  • Place of Presentation: 山形
• [Presentation] スペルミンは熱ショック関連遺伝子群の発現を高めることによりシロイヌナズナを熱ショック傷害から保護する
  • Author(s): Sagor GHM，Berberich Thomas，高橋芳弘，新津 勝，草野友延．
  • Organizer: 日本ポリアミン学会
  • Place of Presentation: 宮城