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Development of Novel Chip for Isolation of Circulating Tumor Cells Using Size-Selection and Photochemical Reaction

Research Project

Project/Area Number 23790023
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Chemical pharmacy
Research InstitutionTokyo University of Science

Principal Investigator

ARIYASU Shinya  東京理科大学, 総合研究機構, ポストドクトラル研究員 (50586998)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords光切断リンカー / 血中循環癌細胞 / 光分解反応 / シリコン基板 / 抗体 / キノリノール
Research Abstract

The isolation of circulating tumor cells (CTCs) is expected toearly-diagnosis and tailor-made care of cancer. However, although the detection of CTCs was reported, the collection of living CTCs have been not realized. In this project, we developed microfluidic device composed of size-selection andIgG-coated photoreactive silicon device. This photoreactive IgG-coated silicon device can capture the target cells from cell-mixture and collect the living target cells by photoirradiation.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (33 results)

All 2013 2012 2011 2010 2009 2008 2007

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (23 results)

  • [Journal Article] The Selective Capture and Collection of Live Target Cells Using a Silicon Wafer Device Modified with Antibodies via a Photocleavable Linker2012

    • Author(s)
      S. Ariyasu, K. Hanaya, E. Watanabe, T. Suzuki, K. Horie, M. Hayase, R. Abe, S. Aoki
    • Journal Title

      Langmuir

      Volume: 28巻 Pages: 13118-13126

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selective Capture and Collection of Live Target Cells Using a Photoreactive Silicon Wafer Device Modified with Antibodies via a Photocleavable Linker2012

    • Author(s)
      Shinya Ariyasu
    • Journal Title

      Langmuir

      Volume: 28 Issue: 36 Pages: 13118-13126

    • DOI

      10.1021/la302393p

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photochemical Cleavage Reaction of 8-Quinolinyl Sulfonates That Are Halogenated and Nitrated at the 7-Position2011

    • Author(s)
      S. Ariyasu, K. Hanaya, M. Tsunoda, M. Kitamura, M. Hayase, R. Abe, S. Aoki
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 59巻 Pages: 1355-1362

    • NAID

      130001274810

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Affinity Clusters: An Adenine-Coated Gold Cluster Binds to Thymine Loops in DNA2011

    • Author(s)
      T. Yamamura, S. Ariyasu, R. Sakamoto
    • Journal Title

      Chemistry an Asian Journal

      Volume: 6巻 Pages: 1761-1765

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Photochemical Cleavage Reaction of 8-Quinolinyl Sulfonates That Are Haloganated and Nitrated at the 7-Position2011

    • Author(s)
      有安 真也、花屋 賢悟、角田めぐみ、北村 正典、早瀬 仁則、安部 良、青木 伸
    • Journal Title

      Chemical & Pharmaceutical Bulletin

      Volume: 59巻 Pages: 1355-1362

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Inorganic Bioconjugates of Gold Clusters and Nanoparticles -Briefly History and Recent Development-2010

    • Author(s)
      T. Yamamura, S. Ariyasu
    • Journal Title

      Bulletin of Japan Coordination Chemistry

      Volume: 55巻 Pages: 71-77

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Alignment of Gold Clusters on DNA via a DNA-recognizing Zinc Finger-Metallothionein Fusion Protein2009

    • Author(s)
      S. Ariyasu, A. Onoda, R. Sakamoto, T. Yamamura
    • Journal Title

      Bioconjugate Chemistry

      Volume: 20巻 Pages: 2278-2285

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Conjugation of Au11 Cluster with Cys-rich Peptides Containing the α-Domain of Metallothionein2009

    • Author(s)
      S. Ariyasu, A. Onoda, R. Sakamoto, T. Yamamura
    • Journal Title

      Dalton Transactions

      Pages: 3742-3747

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Circular Dichroism of Neutral Zinc Porphyrin-Oligonucleotide Conjugates Modified with Flexible Linker2009

    • Author(s)
      A. Onoda, M. Igarashi, S. Naganawa, K. Sasaki, S. Ariyasu, T. Yamamura
    • Journal Title

      Bulletin of the Chemical Society Japan

      Volume: 82巻 Pages: 1280-1286

    • NAID

      10025864717

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Minimal motif peptide structure of metzincin clan zinc peptidases in micelles2009

    • Author(s)
      A. Onoda, T. Suzuki, H. Ishizuka, R. Sugiyama, S. Ariyasu, T. Yamamura
    • Journal Title

      Journal of Peptide Science

      Volume: 15巻 Pages: 832-841

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] p53を標的とする放射線防護剤の探索と機構解析2013

    • Author(s)
      有安真也
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] The Selective Capture and Collection of Live Target Cells Using a Photoreactive Silicon Wafer Device Modified with Antibodies via Photocleavable Linkers2012

    • Author(s)
      S. Ariyasu, K. Hanaya, E. Watanabe, M. Hoshi, T. Suzuki, K. Horie, M. Hayase, R. Abe, S. Aoki
    • Organizer
      13thTetrahedron Symposium Asia Edition (ポスター)
    • Place of Presentation
      中国台湾台北市
    • Related Report
      2012 Final Research Report
  • [Presentation] 光分解可能なケミカルリンカーを用いた目的細胞の選択的捕捉、回収用デバイスの開発(ポスター)2012

    • Author(s)
      有安真也、花屋賢悟、渡邉瑛太、星美里、鈴木利宙、堀江和峰、早瀬仁則、安部良、青木伸
    • Organizer
      第38回反応と合成の進歩シンポジウム
    • Place of Presentation
      東京都
    • Related Report
      2012 Final Research Report
  • [Presentation] 光分解性リンカーを介した抗体修飾デバイスによる目的細胞の選択的捕捉と回収(口頭)2012

    • Author(s)
      有安真也、花屋賢悟、渡邉瑛太、星美里、鈴木利宙、堀江和峰、早瀬仁則、安部良、青木伸
    • Organizer
      第6回バイオ関連化学シンポジウム
    • Place of Presentation
      北海道
    • Related Report
      2012 Final Research Report
  • [Presentation] C3対称性イリジウム錯体を中心骨格とする人工デスリガンドの設計・合成と活性評価2012

    • Author(s)
      渋谷愛
    • Organizer
      第63回有機合成化学協会関東支部シンポジウム
    • Place of Presentation
      東京理科大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] がん細胞のイメージングと細胞死誘導を目的とするシクロメタレート型イリジウム錯体の設計と合成2012

    • Author(s)
      青木伸
    • Organizer
      第22回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      金沢大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of photochemical devices for diagnosis and treatment of cancer2012

    • Author(s)
      青木伸
    • Organizer
      1st International Postgraduate Conference (iPOPs) 2012
    • Place of Presentation
      Puncak Alam
    • Related Report
      2012 Annual Research Report
  • [Presentation] 光分解性リンカーを介した抗体修飾デバイスによる目的細胞の選択的捕捉と回収2012

    • Author(s)
      有安真也
    • Organizer
      第6回バイオ関連化学シンポジウム
    • Place of Presentation
      北海道大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 光分解可能なケミカルリンカーを用いた目的細胞の選択的捕捉、回収用デバイスの開発2012

    • Author(s)
      有安真也
    • Organizer
      第38回反応と合成の進歩シンポジウム
    • Place of Presentation
      船堀タワーホール
    • Related Report
      2012 Annual Research Report
  • [Presentation] The Selective Capture and Collection of Live Target Cells Using a Photoreactive Silicon Wafer Device Modified with Antibodies via Photocleavable Linkers2012

    • Author(s)
      有安真也
    • Organizer
      13th Tetrahedron Symposium Asia Edition
    • Place of Presentation
      台北
    • Related Report
      2012 Annual Research Report
  • [Presentation] 光分解性リンカーを用いた目的細胞の単離・回収用シリコンデバイスの開発2012

    • Author(s)
      有安 真也、花屋 賢悟、星 美里、渡邉 瑛太、鈴木 利宙、早瀬 仁則、安部 良、青木 伸
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] Development of the Specific Antibodies-Coating Photoreactive Silicon Devices for Efficient Detection and Collection of Target Cell (口頭)2011

    • Author(s)
      S. Ariyasu, K. Hanaya, M. Hoshi, E. Watanabe, T. Suzuki, M. Hayase, R. Abe, S. Aoki
    • Organizer
      8th AFMC International Medicinal and Chemical Symposium (AIMECS11)
    • Place of Presentation
      東京都
    • Related Report
      2012 Final Research Report
  • [Presentation] 8-キノリノールスルホン酸エステルの光分解能向上のための官能基導入2011

    • Author(s)
      有安 真也、花屋 賢悟、角田 めぐみ、安部 良、青木 伸
    • Organizer
      第60回有機合成化学協会関東支部シンポジウム
    • Place of Presentation
      千葉大学(千葉県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 二核亜鉛酵素の選択的阻害剤とそのプロドラッグの設計と合成2011

    • Author(s)
      花屋 賢悟、末次 美帆、小林 航也、西條 慎也、有安 真也、北村正典、山登 一郎、青木 伸
    • Organizer
      第21回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      千葉大学(千葉県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Design and Synthesis of a Photocleavable Linker for the Isolation Device of Circulating Tumor Cells (CTCs).2011

    • Author(s)
      有安 真也、花屋 賢悟、渡邊 瑛太、鈴木 利宙、角田 めぐみ、星 美里、北村 正典、早瀬 仁則、安部 良、青木 伸
    • Organizer
      International Symposium on Technologies against Cancer(招待講演)
    • Place of Presentation
      船堀タワーホール(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 8-Quinolinolを母核とする二核亜鉛酵素選択的阻害剤の設計と合成2011

    • Author(s)
      青木 伸、花屋 賢悟、末次 美帆、西條 慎也、小林 航也、水清田 有紀、有安 真也、安部 良、北村 正典、山登 一郎
    • Organizer
      第61回錯体化学討論会
    • Place of Presentation
      岡山理科大学(岡山県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 目的細胞の効率的検出・回収を目的とした光応答性抗体修飾シリコン基板の開発2011

    • Author(s)
      有安 真也、花屋 賢悟、星 美里、渡邉 瑛太、鈴木 利宙、早瀬 仁則、安部 良、青木 伸
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      つくば国際会議場(茨城県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Development of the Specific Antibodies-Coating Photoreactive Silicon Devices for Efficient Detection and Collection of Target Cell2011

    • Author(s)
      有安 真也、花屋 賢悟、星 美里、渡邊 瑛太、鈴木 利宙、早瀬 仁則、安部 良、青木 伸
    • Organizer
      8th AFMC International Medicinal and Chemical Symposium
    • Place of Presentation
      京王プラザホテル(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] Alignment of Gold Clusters on DNA via a Zinc Finger-Metallothionein Fusion Protein (ポスター)2010

    • Author(s)
      S. Ariyasu, T. Yamamura, S. Aoki
    • Organizer
      2010 International Chemical Congress of Pacific Basin Societies (PACICHEM2010)
    • Place of Presentation
      アメリカハワイ州
    • Related Report
      2012 Final Research Report
  • [Presentation] Location of a gold cluster at the desired position of DNA via a zinc finger-metallothionein conjugate (口頭、招待講演)2009

    • Author(s)
      S. Airyasu, R. Sakamoto, T. Yamamura
    • Organizer
      2ndInternational Symposium on Bioinorganic Chemistry of the New Era
    • Place of Presentation
      岐阜県
    • Related Report
      2012 Final Research Report
  • [Presentation] Conjugation of Au11 Cluster with Cys-rich Peptides Containing the α-Domain of Metallothionein (ポスター)2009

    • Author(s)
      S. Ariyasu, A. Onoda, R. Sakamoto, T. Yamamura
    • Organizer
      International Conference on Biological Inorganic Chemistry 2009
    • Place of Presentation
      愛知県
    • Related Report
      2012 Final Research Report
  • [Presentation] Location of a gold cluster at the desired position of DNA via a zinc finger-metallothionein conjugate (ポスター)2008

    • Author(s)
      S. Ariyasu, R. Sakamoto, T. Yamamura
    • Organizer
      International Union of Materials Research Societies-International Conference in Asia 2008
    • Place of Presentation
      愛知県
    • Related Report
      2012 Final Research Report
  • [Presentation] ジンクフィンガー・メタロチオネイン融合蛋白質を用いたAu クラスターのDNA 上への配列(口頭、招待講演)2007

    • Author(s)
      有安真也
    • Organizer
      錯体化学若手の会勉強会
    • Place of Presentation
      東京都
    • Related Report
      2012 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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