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Development of a novel fluorescence derivatization method for the screening of the risk of side effects caused by anticancer drugs

Research Project

Project/Area Number 23790051
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Physical pharmacy
Research InstitutionNagasaki University

Principal Investigator

SHIBATA Takayuki  長崎大学, 医歯薬学総合研究科(薬学系), 助教 (10448491)

Research Collaborator FUJITA Jun-ya  
DRAGUSHA Shpend  
YIN Sheng  
SHIMAMURA Ryosuke  
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsDPD欠損症 / ウラシル / ベンズアミドオキシム / 蛍光反応 / 臨床検査 / スクリーニング / シトシン / オロト酸 / 尿中ウラシル定量 / 核酸代謝 / ハイスループット診断 / ウラシル特異性
Outline of Final Research Achievements

In this research, a uracil-specific fluorogenic reaction developed by our research group was applied to the quantification of urinary uracil concentration for the development of a facile and rapid diagnostic method of DPD deficiency. As a result, we have succeeded in a simultaneous analysis of urinary uracil level using multiple samples, which requires a simple addition of aqueous reagent solutions and heating for a few minutes. The result suggested that the present method would be applicable to the screening test of DPD deficiency at a clinical site. In addition, by changing the reaction conditions of the uracil-specific fluorogenic reaction, we also have discovered novel fluorescence derivatization methods specific to cytosine and orotic acid, respectively.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (57 results)

All 2015 2014 2013 2012 2011 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (41 results) (of which Invited: 2 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Facile preparation of streptavidin-coated sephadex beads and their application to chemiluminescence detection of a target DNA2015

    • Author(s)
      El-Mahdy AF, Ejupi V, Shibata T, Kabashima T, Lu J, Kai M
    • Journal Title

      Mcrochimica Acta

      Volume: 182 Issue: 3-4 Pages: 495-503

    • DOI

      10.1007/s00604-014-1348-9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A Novel Fluorometric Method for the Selective Determination of Pro-Gly and Pro-Gly-Pro2014

    • Author(s)
      Yasmin H, Rahman MS, Shibata T, Kabashima T, Kai M
    • Journal Title

      International Journal of Peptide Research and Therapeutics

      Volume: 20 Issue: 4 Pages: 441-446

    • DOI

      10.1007/s10989-014-9406-z

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Amplified and selective assay of collagens by enzymatic and fluorescent reactions2014

    • Author(s)
      Yasmin H, Kabashima T, Rahman MS, Shibata T, Kai M
    • Journal Title

      Scientific Reports

      Volume: 4 Issue: 1 Pages: 4950-4950

    • DOI

      10.1038/srep04950

    • NAID

      120006986568

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Carbon Nanofiber-based Luminol-biotin Probe for Sensitive Chemiluminescence Detection of Protein2014

    • Author(s)
      Baj S, Krawczyk T, Pradel N, Azam MG, Shibata T, Dragusha S, Skutil K, Pawlyta M, Kai M
    • Journal Title

      Analytical Sciences

      Volume: 30 Issue: 11 Pages: 1051-1056

    • DOI

      10.2116/analsci.30.1051

    • NAID

      130004704337

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dendrimer-like polymeric DNAs as chemiluminescence probes for amplified detection of telomere DNA on a solid-phase membrane2014

    • Author(s)
      El-MandyAF, Shibata T, Kabashima T, Kai M
    • Journal Title

      Chem. Commun.

      Volume: 50 Issue: 7 Pages: 859-861

    • DOI

      10.1039/c3cc47454b

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Selective, sensitive and fluorometric determination of urinary cytosine with 4-trifluoromethylbenzamidoxime and N, N-dimethylformamide2014

    • Author(s)
      Dragusha S, Shibata T, Yin S, Fujita JY, Kabashima T, Kai M
    • Journal Title

      Clin. Chim. Acta

      Volume: 429 Pages: 123-128

    • DOI

      10.1016/j.cca.2013.11.027

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Selective FL quenching or enhancing of diimine ligands by guanine2013

    • Author(s)
      Smanmoo S, Kawasaki S, Tangboriboonrat P, Shibata T, Kabashima T, Kai M
    • Journal Title

      J. Fluoresc.

      Volume: 23 Issue: 5 Pages: 853-857

    • DOI

      10.1007/s10895-013-1216-8

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A novel fluorescence reaction for N-terminal Ser-containing peptides and its application to assay caspase activity2013

    • Author(s)
      Rahman MS, Kabashima T, Yasmin H, Shibata T, Kai M
    • Journal Title

      Anal. Biochem.

      Volume: 433 Issue: 2 Pages: 79-85

    • DOI

      10.1016/j.ab.2012.10.018

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Inhibition of HIV-1 protease expression in T cells owing to DNA aptamer-mediated specific delivery of s1RNA2012

    • Author(s)
      Zhu Q, Shibata T, Kabashima T, Kai M
    • Journal Title

      Eur. J. Med. Chem.

      Volume: 56 Pages: 396-399

    • DOI

      10.1016/j.ejmech.2012.07.045

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Selective and sensitive determination of peptides using 3, 4-dihydroxyphenylacetic acid as a fluorogenic reagent2012

    • Author(s)
      Yasmin H, Shibata T, Rahman MS, Kabashima T, Kai M
    • Journal Title

      Anal. Chim. Acta

      Volume: 721 Pages: 162-166

    • DOI

      10.1016/j.aca.2012.01.035

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Alkaline phosphatase-labeled macromolecular probe for sensitive chemiluminescence detection of proteins on a solid-phase membrane2011

    • Author(s)
      Azam MG, Shibata T, Kabashima T, Kai M
    • Journal Title

      Anal. Bioanal. Chem.

      Volume: 401 Issue: 4 Pages: 1211-1217

    • DOI

      10.1007/s00216-011-5196-8

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sensitive Chemiluminescence Detection of Prion Protein on a Membrane by Using a Peroxidase-Labeled Dextran Probe2011

    • Author(s)
      MG.Azam
    • Journal Title

      Analytical Sciences

      Volume: 27 Issue: 7 Pages: 715

    • DOI

      10.2116/analsci.27.715

    • NAID

      10029332555

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A manual sequence method of peptides and phosphopeptides using 4-(1'-cyanoisoindolyl)phenyliso thiocyanate2011

    • Author(s)
      Shibata T, Wainaina MN, Miyoshi T, Kabashima T, Kai M
    • Journal Title

      J. Chromatogr. A

      Volume: 1218 Issue: 24 Pages: 3757-3762

    • DOI

      10.1016/j.chroma.2011.04.040

    • NAID

      120006985100

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chemiluminescence detection of telomere DNA in human cells on a membrane by using fluorescein-5-isothiocyanate-labeled primers2011

    • Author(s)
      Yamasuji M, Shibata T, Kabashima T, Kai M
    • Journal Title

      Anal. Biochem.

      Volume: 413 Issue: 1 Pages: 50-54

    • DOI

      10.1016/j.ab.2011.01.047

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 3,4-DHPAA 蛍光反応の新規プロリダーゼ活性測定法への応用2015

    • Author(s)
      椛島 力, 曽宮実和子, 柴田孝之, 甲斐雅亮
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸学院大学, 神戸サンボーホール, 兵庫医療大学, デザイン・クリエイティブセンター神戸(神戸市)
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] ペプチドに特異的な蛍光反応を用いたHIV/HCV識別法の開発2014

    • Author(s)
      川島歌織, 椛島 力, 柴田孝之, 甲斐雅亮
    • Organizer
      第31回日本薬学会九州支部大会
    • Place of Presentation
      第一薬科大学(福岡市)
    • Year and Date
      2014-12-06 – 2014-12-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 3,4-DHPAA を用いた新規プロリダーゼ活性測定法の開発2014

    • Author(s)
      曽宮実和子, 椛島 力, 柴田孝之, 甲斐雅亮
    • Organizer
      第31回日本薬学会九州支部大会
    • Place of Presentation
      第一薬科大学(福岡市)
    • Year and Date
      2014-12-06 – 2014-12-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dihydroorotate dehydrogenase assay using a specific fluorescence reaction2014

    • Author(s)
      尹 晟, 椛島 力, 柴田孝之, 甲斐雅亮
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学(東広島市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Assay of collagenase activity in human cells using a novel fluorescence reaction2014

    • Author(s)
      Valon Ejupi, 椛島 力, 柴田孝之, 甲斐雅亮
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学(東広島市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Chemiluminescence detection of telomere DNA by using dendrimer-like polymeric DNAs2013

    • Author(s)
      Ahmed El-Mahdy, Takayuki Shibata, Tsutomu Kabashima, Masaaki Kai
    • Organizer
      第26回バイオメディカル分析科学シンポジウム(BMAS2013)
    • Place of Presentation
      昭和大学(東京都)
    • Related Report
      2013 Research-status Report
  • [Presentation] A novel and specific fluorescence reaction for orotic acid2013

    • Author(s)
      Sheng Yin, Takayuki Shibata, Shpend Dragusha, Jun-ya Fujita, Tsutomu Kabashima, Masaaki Kai
    • Organizer
      The Twelfth Asian Conference on Analytical Sciences (ASIANALYSIS XII)
    • Place of Presentation
      九州大学(福岡市)
    • Related Report
      2013 Research-status Report
  • [Presentation] A multi-substrate fluorometric assay for HIV-1 protease activity and its application in drug resistance detection2013

    • Author(s)
      Qinchang Zhu, Tsutomu Kabashima, Takayuki Shibata, Masaaki Kai
    • Organizer
      2013 China-Japan-Korea Symposium on Analytical Chemistry (CJK 2013)
    • Place of Presentation
      九州大学および長崎国際大学(福岡市および佐世保市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Fundamental Conditions for assay of collagenase activity using a novel fluorescence reaction2013

    • Author(s)
      Valon Ejupi, Tsutomu Kabashima, Hasina Yasmin, Shpend Dragusha, Masaaki Kai
    • Organizer
      日本分析化学会第62年会
    • Place of Presentation
      近畿大学(東大阪市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 薬剤耐性を示すHIV-1プロテアーゼの新規蛍光識別法の開発2013

    • Author(s)
      椛島力、朱欽昌、川島歌織、矢ヶ部良太、柴田孝之、甲斐雅亮
    • Organizer
      第54回日本熱帯医学会大会
    • Place of Presentation
      長崎大学(長崎市)
    • Related Report
      2013 Research-status Report
  • [Presentation] プロテアーゼ抵抗性プリオンタンパク質の酵素分解を促進する薬剤の探索2013

    • Author(s)
      永本佳菜子、椛島力、陣内伸俊、 森薗文恵、柴田孝之、甲斐雅亮
    • Organizer
      第30回日本薬学会九州支部大会
    • Place of Presentation
      長崎国際大学(佐世保市)
    • Related Report
      2013 Research-status Report
  • [Presentation] FITC修飾を施した二本鎖核酸の細胞膜透過性2013

    • Author(s)
      和田怜、坂元謙太、椛島力、柴田孝之、甲斐雅亮
    • Organizer
      第30回日本薬学会九州支部大会
    • Place of Presentation
      長崎国際大学(佐世保市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Dendrimer-like polymeric DNAs as a chemiluminescence probe2013

    • Author(s)
      Ahmed El-Mahdy, Takayuki Shibata, Tsutomu Kabashima, Masaaki Kai
    • Organizer
      第30回日本薬学会九州支部大会
    • Place of Presentation
      長崎国際大学(佐世保市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Inhibition of the expression of HIV-1 protease in CD4+ T cells owing to DNA aptamer-mediated specific delivery of siRNA2012

    • Author(s)
      Qinchang Zhu
    • Organizer
      The 6th Nagasaki Symposium on Tropical and Emerging Infectious Diseases and The 11th Nagasaki-Singapore Medical Symposium
    • Place of Presentation
      長崎大学(長崎市)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 真核細胞発現系を用いたプリオンタンパク質の発現と精製2012

    • Author(s)
      中村 祐介
    • Organizer
      第29回日本薬学会九州支部大会
    • Place of Presentation
      熊本大学(熊本市)
    • Related Report
      2012 Research-status Report
  • [Presentation] マイクロチップ電気泳動を用いた迅速なペプチド検出法の開発2012

    • Author(s)
      松木 勝仁
    • Organizer
      第29回日本薬学会九州支部大会
    • Place of Presentation
      熊本大学(熊本市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Highly selective and sensitive method for the determination of collagen in mammalian tissue by a novel fluorescence reaction2012

    • Author(s)
      Masaaki Kai
    • Organizer
      The 6th Shanghai international symposium on analytical chemistry
    • Place of Presentation
      上海市(中国)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] DNA aptamer-mediated siRNA delivery inhibits the expression of HIV-1 protease in T cells2012

    • Author(s)
      Qinchang Zhu
    • Organizer
      2012 China-Japan-Korea symposium on analytical chemistry
    • Place of Presentation
      上海市(中国)
    • Related Report
      2012 Research-status Report
  • [Presentation] A novel fluorescence derivatization reaction specific for cytosine2012

    • Author(s)
      Dragusha Shpend
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      金沢大学(金沢市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 真核細胞における組換えHIV-1プロテアーゼの発現と検出法の開発2012

    • Author(s)
      永本 佳菜子
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      金沢大学(金沢市)
    • Related Report
      2012 Research-status Report
  • [Presentation] プロテアーゼ抵抗性プリオンタンパク質の酵素分解の促進2012

    • Author(s)
      室田 紗由美
    • Organizer
      第25回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      慶応義塾大学(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] The potential application of DNA aptamer-mediated specific delivery of siRNA in anti-HIV therapy2012

    • Author(s)
      Qinchang Zhu
    • Organizer
      第25回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      慶応義塾大学(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] HIV-1プロテアーゼの発現プラスミドの作製と真核細胞での発現2012

    • Author(s)
      永本 佳菜子
    • Organizer
      第30回九州分析化学若手の会夏季セミナー
    • Place of Presentation
      休暇村指宿(指宿市)
    • Related Report
      2012 Research-status Report
  • [Presentation] A fluorescence reaction for the sensitive quantification of orotic acid2012

    • Author(s)
      尹 晟
    • Organizer
      第30回九州分析化学若手の会夏季セミナー
    • Place of Presentation
      休暇村指宿(指宿市)
    • Related Report
      2012 Research-status Report
  • [Presentation] A highly sensitive and quantitative assay for collagen in biological sample by a novel fluorescence reaction2012

    • Author(s)
      Hasina Yasmin
    • Organizer
      第72回分析化学討論会
    • Place of Presentation
      鹿児島大学(鹿児島市)
    • Related Report
      2012 Research-status Report
  • [Presentation] プロテアーゼ抵抗性プリオンタンパク質のプロテアーゼ分解における界面活性剤の効果2012

    • Author(s)
      室田 紗由美
    • Organizer
      第72回分析化学討論会
    • Place of Presentation
      鹿児島大学(鹿児島市)
    • Related Report
      2012 Research-status Report
  • [Presentation] A novel fluorescence reaction for N-terminal serine-containing peptides and its application to assay of caspase activity2012

    • Author(s)
      Mohammed Shafikur Rahman
    • Organizer
      第72回分析化学討論会
    • Place of Presentation
      鹿児島大学(鹿児島市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 核酸輸送のための蛍光物質の細胞膜透過性

    • Author(s)
      坂元謙太、椛島力、柴田孝之、甲斐雅亮
    • Organizer
      第50 回化学関連支部合同九州大会
    • Place of Presentation
      北九州国際会議場およびAIMビル(北九州市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 新規蛍光反応を用いたウイルス識別法の開発

    • Author(s)
      川島歌織、椛島力、朱欽昌、永本佳菜子、矢ヶ部良太、柴田孝之、甲斐雅亮
    • Organizer
      第50 回化学関連支部合同九州大会
    • Place of Presentation
      北九州国際会議場およびAIMビル(北九州市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 4-Trifluorobenzamidoxime as a specific fluorogenic reagent for cytosine

    • Author(s)
      Shpendo Dragusha、柴田 孝之、藤田 順也、尹 晟、椛島 力、甲斐 雅亮
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2011 Research-status Report
  • [Presentation] A fluorescence reaction for the sensitive quantification of orotic acid

    • Author(s)
      尹 晟、柴田 孝之、藤田 順也、Shpendo Dragusha、椛島 力、甲斐 雅亮
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2011 Research-status Report
  • [Presentation] Inhibition of HIV-1 protease expression in T cells using CD4 aptamer-siRNA chimeras

    • Author(s)
      朱 欽昌、柴田 孝之、椛島 力、甲斐 雅亮
    • Organizer
      第28回日本薬学会九州支部大会
    • Place of Presentation
      福岡大学(福岡市)
    • Related Report
      2011 Research-status Report
  • [Presentation] タンパク質マルチラベル化高分子の合成及び化学発光検出プローブとしての応用

    • Author(s)
      西村 沙也加、柴田 孝之、Md. Golam Azam、椛島 力、甲斐 雅亮
    • Organizer
      生物発光化学発光研究会第28回学術講演会
    • Place of Presentation
      長崎大学(長崎市)
    • Related Report
      2011 Research-status Report
  • [Presentation] カスパーゼ3および8活性の新規蛍光測定法の開発

    • Author(s)
      Rahman Mohammed Shafikur、椛島 力、Yasmin Hasina、柴田 孝之、甲斐 雅亮
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Related Report
      2011 Research-status Report
  • [Presentation] Determination of collagen in mammalian tissue by a novel fluorescence reaction

    • Author(s)
      Yasmin Hasina、柴田 孝之、Eahman Mohammed Shafikur、椛島 力、甲斐 雅亮
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 3-メチルベンズアミドオキシムを用いた尿中ウラシル定量法およびジヒドロピリミジン脱水素酵素欠損症診断への応用

    • Author(s)
      藤田 順也、柴田 孝之、Akhoondi Reza、椛島 力、甲斐 雅亮
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 蛍光誘導体化反応と電気泳動を組み合わせたペプチドの迅速な分離

    • Author(s)
      松木 勝仁、朱 欽昌、椛島 力、柴田 孝之、甲斐 雅亮
    • Organizer
      第24回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      大山ロイヤルホテル(鳥取県西伯郡伯耆町)
    • Related Report
      2011 Research-status Report
  • [Presentation] 酵素分解に抵抗性を示すプリオンタンパク質(PrP)の作製と真核細胞でのPrPの発現

    • Author(s)
      中村 祐介、室田 紗由美、近藤 緑、白木 里沙、椛島 力、柴田 孝之、甲斐 雅亮
    • Organizer
      第24回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      大山ロイヤルホテル(鳥取県西伯郡伯耆町)
    • Related Report
      2011 Research-status Report
  • [Presentation] siRNAの分解及び核酸塩基の修飾による分解抵抗性の獲得

    • Author(s)
      小代 昌平、柴田 孝之、椛島 力、甲斐 雅亮
    • Organizer
      第29回九州分析化学若手の会 夏季セミナー
    • Place of Presentation
      国民宿舎めかり山荘(北九州市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 2環状デオキシシチジンを有する人工siRNAの合成研究

    • Author(s)
      和田 怜、黒川 史子、柴田 孝之、椛島 力、甲斐 雅亮
    • Organizer
      第29回九州分析化学若手の会 夏季セミナー
    • Place of Presentation
      国民宿舎めかり山荘(北九州市)
    • Related Report
      2011 Research-status Report
  • [Presentation] ウラシル特異的な蛍光反応によるDPD欠損の簡易診断

    • Author(s)
      柴田 孝之
    • Organizer
      第10回国際バイオEXPO
    • Place of Presentation
      東京ビッグサイト(東京都)
    • Related Report
      2011 Research-status Report
  • [Patent(Industrial Property Rights)] URACIL-SPECIFIC FLUORESCENCE DETECTION REACTION AND METHOD FOR EXAMINING DIHYDROPYRIMIDINE DEHYDROGENASE DEFICIENCY2013

    • Inventor(s)
      Masaaki Kai, Takayuki Shibata
    • Industrial Property Rights Holder
      Masaaki Kai, Takayuki Shibata
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-02-28
    • Acquisition Date
      2014-10-28
    • Related Report
      2014 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] ペプチドの検出方法2011

    • Inventor(s)
      甲斐雅亮、椛島力、柴田孝之
    • Industrial Property Rights Holder
      甲斐雅亮、椛島力、柴田孝之
    • Filing Date
      2011
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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