Molecular analysis of SH3P2 function during cell migration

• Project/Area Number: 23790090
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Nagasaki University
• Principal Investigator: TANIMURA Susumu 長崎大学, 大学院・医歯薬学総合研究科, 助教 (90343342)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 癌 / シグナル伝達 / 細胞運動 / リン酸化 / MAPキナーゼ / スプライシング

Research Abstract

SH3P2, a novel regulator of cell migration, binds to hnRNP-K and Myosin1E. Overexpression of SH3P2 inhibited the splicing of MMP-3/-9 mRNA. GST pull down/LC-MS/MS assay revealed that SH3 domain of Myosin1E interacts with Caveolin1-Cavin complex, which plays important roles in caveolae formation. These results indicate that SH3P2 regulates cell migration via hnRNP-K-mediated mRNA splicing of MMPs and Myosin1E-mediated caveolae formation

Research Products

• [Journal Article] Blockade of the ERK pathway enhances the therapeutic efficacy of the histone deacetylase inhibitor MS-275 in human tumor xenograft models. (2013)
  • Author(s): Sakamoto, T.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 433 Issue: 4 Pages: 456-462
  • DOI: 10.1016/j.bbrc.2013.03.009
  • Peer Reviewed
• [Journal Article] Central role of the exchange factor GEF-H1 in TNF-α–induced sequential activation of Rac, ADAM17/TACE, and RhoA in tubular epithelial cells (2013)
  • Author(s): Waheed, F.
  • Journal Title: Mol. Biol. Cell Volume: 24 Issue: 7 Pages: 1068-1082
  • DOI: 10.1091/mbc.e12-09-0661
  • Peer Reviewed
• [Journal Article] Functional characterization of the novel BRAF complex mutation, BRAF(V600delinsYM), identified in papillary thyroid carcinoma. (2013)
  • Author(s): Matsuse, M.
  • Journal Title: Int. J. Cancer Volume: 132 Issue: 3 Pages: 738-743
  • DOI: 10.1002/ijc.27709
  • Peer Reviewed
• [Journal Article] Up-regulation of pro-apoptotic protein Bim and down-regulation of anti-apoptotic protein Mcl-1 cooperatively mediate enhanced tumor cell death induced by the combination of ERK kinase (MEK) inhibitor and microtubule inhibitor (2012)
  • Author(s): Kawabata, T., Kohno, M., et al
  • Journal Title: J.Biol.Chem. Volume: 287 Issue: 13 Pages: 10289-10300
  • DOI: 10.1074/jbc.m111.319426
  • Peer Reviewed
• [Journal Article] Up-regulation of Pro-apoptotic Protein Bim and Down-regulation of Anti-apoptotic protein Mcl-1 Cooperatively Mediate Enhanced Tumor Cell Death Induced by the Combination of ERK Kinase (MEK) Inhibitor and Microtubule inhibitor. (2012)
  • Author(s): Takumi Kawabata et al.
  • Journal Title: J. Biol. Chem. Volume: 289 Pages: 10289-10300
  • Peer Reviewed
• [Journal Article] New microtubule polymerization inhibitors comprising a nitrooxymethylphenyl group (2011)
  • Author(s): Y.Kawaratani, T.Harada, Y.Hirata, Y.Nagaoka, S.Uesato, 他5名
  • Journal Title: Bioorg.Med.Chem. Volume: 19 Issue: 13 Pages: 3995-4003
  • DOI: 10.1016/j.bmc.2011.05.031
  • Peer Reviewed
• [Journal Article] Targeting the extracellular signal-regulated kinase pathway in cancer therapy (2011)
  • Author(s): Kohno, M., Tanimura, S., Ozaki, K
  • Journal Title: Biol. Pharm. Bull Volume: 34 Pages: 1781-1784
  • NAID: 130001872595
• [Journal Article] New microtubule polymerization inhibitors comprising a nitrooxymethylphenyl group. (2011)
  • Author(s): Yasuyuki Kawaratani et al.
  • Journal Title: Bioorg. Med. Chem. Volume: 19 Pages: 3995-4003
  • Peer Reviewed
• [Journal Article] Targeting the extracellular signal-regulated kinase pathway in cancer therapy. (2011)
  • Author(s): Michiaki Kohno et al.
  • Journal Title: Biol. Pharm. Bull. Volume: 34 Pages: 1781-1784
• [Presentation] Myosin1Eの細胞運動亢進作用はUNC45-Hsp90複合体による細胞内局在制御によって調節される (2012)
  • Author(s): 谷村進
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 2012-12-15
• [Presentation] UNC45によるMyosin1Eの細胞内局在制御 (2012)
  • Author(s): 平田弦也
  • Organizer: 第29回日本薬学会九州支部大会
  • Place of Presentation: 熊本大学薬学部
  • Year and Date: 2012-12-09
• [Presentation] Myosin1Eによるカベオラ形成制御と細胞運動 (2012)
  • Author(s): 中原康子
  • Organizer: 第29回 日本薬学会九州支部大会
  • Place of Presentation: 熊本大学薬学部
  • Year and Date: 2012-12-09
• [Presentation] MEK and microtubule inhibitors together kill tumor cells through up-regulation of Bim and down-regulation of Mcl-1 (2012)
  • Author(s): 谷村進
  • Organizer: 第71回日本癌学会学術大会
  • Place of Presentation: ロイトン札幌
  • Year and Date: 2012-09-20
• [Presentation] Myosin1Eによる細胞運動制御の分子メカニズム (2011)
  • Author(s): 森田和幹
  • Organizer: 第28回日本薬学会九州支部大会
  • Place of Presentation: 福岡大学
  • Year and Date: 2011-12-10
• [Presentation] Myosin1E enhances cell motility via the induction of filopodia formation: essential role of phosphorylation on Ser736 (2011)
  • Author(s): 谷村進
  • Organizer: 第70回日本癌学会学術大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-05
• [Presentation] Myosin 1E enhances cell motility via the induction of filopodia formation: essential role of phosphorylation on Ser736 (2011)
  • Author(s): 谷村 進
  • Organizer: 第70回 日本癌学会学術大会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] Myosin 1E による細胞運動制御の分子メカニズム (2011)
  • Author(s): 森田 和幹
  • Organizer: 第28回 日本薬学会九州支部大会
  • Place of Presentation: 福岡大学
• [Presentation] MEK and microtubule inhibitors together kill tumor cells through up-regulation of Bim and down-regulation of Mcl-1.
  • Author(s): 谷村 進
  • Organizer: 第71回 日本癌学会学術大会
  • Place of Presentation: ロイトン札幌
• [Presentation] Myosin1Eの細胞運動亢進作用はUNC45-Hsp90複合体による細胞内局在制御によって調節される
  • Author(s): 谷村 進
  • Organizer: 第85回 日本生化学会大会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] UNC45によるMyosin1Eの細胞内局在制御
  • Author(s): 平田弦也
  • Organizer: 第29回 日本薬学会九州支部大会
  • Place of Presentation: 熊本大学薬学部