Generation of pancreatic beta cell from human pluripotent stem cell by use of the purification with flow cytometry

Research Project

Project/Area Number	23791025
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Metabolomics
Research Institution	Kyoto University
Principal Investigator	TOYODA Taro 京都大学, iPS 細胞研究所, 特定拠点助教 (60593530)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000) Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	再生医学 / エネルギー / 糖質代謝異常 / PDX1 / 膵臓β細胞 / インスリン / 糖尿病 / iPS 細胞 / 膵臓 / β細胞 / iPS細胞
Research Abstract	Cell therapy using pancreatic ・cells generated from human pluripotent stem cells (hPSCs) is one of the possible solutionsof diabetes problems. Since the generation of target cell types from PSCs with high purity is currently difficult, it is necessary to isolate differentiated pancreatic cells for accurate analysis. In this study, we generated a human iPS cell line in which the fluorescent protein reporter gene is expressed under the control of the promoter of PDX1 gene, a key transcriptional factor for pancreatic development and function. This cell line is useful to analyze pancreatic cells derived from hPSCs

Report

(3 results)