| Project/Area Number |
23791211
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | National Research Institute for Child Health and Development |
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Principal Investigator |
IIJIMA Kazutoshi 独立行政法人国立成育医療研究センター, 小児血液・腫瘍研究部, 共同研究員 (30468508)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | バーキットリンパ腫 / アポトーシス / 胚中心 / リンパ腫 / B細胞 |
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| Research Abstract |
ZNF385B is a zinc finger protein that we previously identified as a molecule specifically expressed in BL using gene expression analyses. The biological significance of this protein has not been clarified at all. Therefore, we intended to elucidate diagnostic prospects and isoform-dependent functions of ZNF385B in B cells. Ectopic expression of ZNF385B IF-1 induced up-regulation of PERP (p53 apoptosis effector related to PMP-22) and activation of caspase-3 and -8, resulting in apoptosis induction, whereas IF-1/DEL did not. Furthermore, IF-1/DEL inhibited apoptosis induced by CD20 and BCR stimulation. Immunoprecipitation and yeast two-hybrid analysis indicated the direct binding of ZNF385B with p53. Since PERP is known to be a p53 transcriptional target, these results suggest the involvement of ZNF385B in B-cell apoptosis by modulating p53 transactivation. ZNF385B is considered to be involved in the regulation of death and survival that specifically occurs in GC B cells.
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