The isolation and characterization of cancer associated fibroblasts for clinical therapeutic application.
Project/Area Number |
23791549
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Digestive surgery
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Research Institution | Kumamoto University |
Principal Investigator |
OKABE Hirohisa 熊本大学, 医学部附属病院, 非常勤診療医師 (40573621)
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Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | CAF(癌関連線維芽細胞) / 胆管癌 / 肝星細胞 / myofibroblast / 組織消化 / 癌関連線維芽細胞 / 肝内胆管癌 / 線維芽細胞 |
Research Abstract |
The aim of this study is to isolate cancer-associated fibroblasts (CAFs) and characterize them in cholangiocarcinoma. In addition, we aimed to isolate normal fibroblasts to find the difference from CAFs considering therapeutic application. Since the tissue of human cholanigocarcinoma was quite difficult to digest and the number of patients was limited, we decided to get myofibroblasts form chronic inflammatory liver and normal fibroblasts from normal liver using mouse model. We performed liver perfusion and optimized the digestion buffer. We made the protocol of cell separation for both populations.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Role of leukocyte cell-derived chemotaxin 2 as a biomarker in hepatocellular carcinoma2014
Author(s)
Okabe H, Delgado E, Lee JM, Yang J, Kinoshita H, Hayashi H, Tsung A, Behari J, Beppu T, Baba H, Monga SP.
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Journal Title
Plos One
Volume: (in press)
Related Report
Peer Reviewed
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[Journal Article] Rank classification based on the combination of indocyanine green retention rate at 15 min and (99m)Tc-DTPA-galactosyl human serum albumin scintigraphy predicts the safety of hepatic resection2014
Author(s)
Okabe H, Beppu T, Hayashi H, Masuda T, Mima K, Nakagawa S, Kuroki H, Imai K, Nitta H, Masuda T, Hashimoto D, Chikamoto A, Wanatanabe M, Ishiko T, Yoshida M, Yamashita Y, Baba H.
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Journal Title
Nucl Med Commun
Volume: 35
Issue: 5
Pages: 478-483
DOI
Related Report
Peer Reviewed
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[Journal Article] Macrophage-derived reactive oxygen species suppress miR-328 targeting CD44 in cancer cells and promote redox adaptation2014
Author(s)
Ishimoto T, Sugihara H, Watanabe M, Sawayama H, Iwatsuki M, Baba Y, Okabe H, Hidaka K, Yokoyama N, Miyake K, Yoshikawa M, Nagano O, Komohara Y, Takeya M, Saya H, Baba H.
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Journal Title
Carcinogenesis
Volume: 35(5)
Issue: 5
Pages: 1003-11
DOI
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Peer Reviewed / Open Access
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[Journal Article] CD44s signals the acquisition of the mesenchymal phenotype required for anchorage-independent cell survival in hepatocellular carcinoma.2014
Author(s)
Okabe H, Ishimoto T, Mima K, Nakagawa S, Hayashi H, Kuroki H, Imai K, Nitta H, Saito S, Hashimoto D, Chikamoto A, Ishiko T, Watanabe M, Nagano O, Beppu T, Saya H and Baba H
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Journal Title
Br J Cancer
Volume: 印刷中
Issue: 4
Pages: 958-66
DOI
Related Report
Peer Reviewed
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[Journal Article] Macrophage-derived reactive oxygen species suppress miR-328 targeting CD44 in cancer cells and promote redox adaptation.2014
Author(s)
Ishimoto T, Sugihara H, Watanabe M, Sawayama H, Iwatsuki M, Baba Y, Okabe H, Hidaka K, Yokoyama N, Miyake K, Yoshikawa M, Nagano O, Komohara Y, Takeya M, Saya H, Baba H.
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Journal Title
Carcinogenesis
Volume: In press
Related Report
Peer Reviewed
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[Journal Article] Epigenetic therapy with the histone methyltransferase EZH2 inhibitor 3-deazaneplanocin A inhibits the growth of cholangiocarcinoma cells.2014
Author(s)
Nakagawa S, Sakamoto Y, Okabe H, Hayashi H, Hashimoto D, Yokoyama N, Tokunaga R, Sakamoto K, Kuroki H, Mima K, Beppu T, Baba H.
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Journal Title
Oncology Report
Volume: 31
Issue: 2
Pages: 983-8
DOI
Related Report
Peer Reviewed
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[Journal Article] Remnant liver volume-based predictors of postoperative liver dysfunction after hepatectomy : Analysis of 625 consecutive patients from a single institution2013
Author(s)
Okabe H, Beppu T, Chikamoto A, Hayashi H, Yoshida M, Masuda T, Imai K, Mima K, Nakagawa S, Kuroki H, Nitta H, Hashimoto D, Ishiko T, Yamashita Y, Baba H.
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Journal Title
Int J Clin Oncol
Volume: (Epub ahead of print)
Issue: 4
Pages: 614-21
DOI
Related Report
Peer Reviewed
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[Journal Article] Percentage of future liver remnant volume before portal vein embolization influences the degree of liver regeneration after hepatectomy. J Gastrointest Surg2013
Author(s)
Okabe H, Beppu T, Nakagawa S, Yoshida M, Hayashi H, Masuda T, Imai K, Mima K, Kuroki H, Nitta H, Hashimoto D, Chikamoto A, Ishiko T, Watanabe M, Yamashita Y, Baba H.
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Journal Title
J Gastrointest Surg
Volume: 17(8)
Issue: 8
Pages: 1447-51
DOI
Related Report
Peer Reviewed
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[Journal Article] Mima K, Hayashi H, Kuroki H, NakagawaS, Okabe H, Chikamoto A, Watanabe M,Beppu T, Baba H2013
Author(s)
Mima K, Hayashi H, Kuroki H, NakagawaS, Okabe H, Chikamoto A, Watanabe M,Beppu T, Baba H
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Journal Title
Oncology Lett
Volume: 5
Issue: 1
Pages: 149-154
DOI
Related Report
Peer Reviewed
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[Journal Article] High CD44s expression is associatedwith the EMT expression profile andintrahepatic dissemination ofhepatocellular carcinoma after localablation therapy.2013
Author(s)
Mima K, Hayashi H,Imai K, Kuroki H,Shigaki N, Nakagawa S, Okabe H,Chikamoto A, Watanabe M, Beppu T, BabaH
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Journal Title
J Hepatobiliary Pancreatic Surg
Volume: 20
Issue: 4
Pages: 429-34
DOI
Related Report
Peer Reviewed
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[Journal Article] CYLD downregulation is correlated with tumor development in patients with hepatocellular carcinoma2013
Author(s)
Kinoshita H, Okabe H, Beppu T, Chikamoto A, Hayashi H, Imai K, Mima K, Nakagawa S, Yokoyama N, Ishiko T, Shinriki S, Jono H, Ando Y, Baba H.
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Journal Title
Mol Clin Oncol
Volume: 1
Pages: 309-314
URL
Related Report
Peer Reviewed
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[Journal Article] Kinoshita H, Okabe H, Beppu T,Chikamoto A, Hayashi H, Imai K, MimaK, Nakagawa S, Ishimoto T, Miyake K,Yokoyama N, Ishiko T, Baba2012
Author(s)
Kinoshita H, Okabe H, Beppu T,Chikamoto A, Hayashi H, Imai K, MimaK, Nakagawa S, Ishimoto T, Miyake K,Yokoyama N, Ishiko T, Baba
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Journal Title
Oncol Rep
Volume: 29
Issue: 2
Pages: 685-9
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of CXCL5/ENA-78 as afactor involved in the interactionbetween cholangiocarcinoma cells andcancer-associated fibroblasts.2012
Author(s)
Okabe H, Beppu T, Ueda M, Hayashi H,Ishiko T, Masuda T, Otao R, Horlad H,Mima K, Miyake K, Iwatsuki M, Baba Y,Takamori H, Jono H, Shinriki S, AndoY, Baba H
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Journal Title
Int J Cancer 131
Volume: in press
Issue: 10
Pages: 2234-41
DOI
Related Report
Peer Reviewed
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[Journal Article] Hepatic stellate cells acceleratethe malignant behavior ofcholangiocarcinoma.2011
Author(s)
DOI:10.5772/51793kabe H, Beppu T, Hayashi H, Ishiko T,Masuda T, Otao R, Shinriki S, HorladH, Jono H, Ueda M, Ando Y, Baba H
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Journal Title
Ann Surg Oncol
Volume: 18
Issue: 4
Pages: 1175-84
DOI
Related Report
Peer Reviewed
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[Presentation] Identification of CXCL5/ENA-78 regulating the interaction of cholangiocarcinoma and cancer-associated fibroblasts2012
Author(s)
Okabe H, Beppu T, Ueda M, Hayashi H, Masuda T, Mima K, Nakagawa S, Miyake K, Yokoyama N, Baba Y, Takamori H, Ishiko T, Jono H, Shinriki S, Ando Y, Baba H.
Organizer
AACR103th Annual Meeting 2012
Place of Presentation
Chicago convention center, IL, USA
Related Report
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[Presentation] Side population cells have some stem-like properties in hepatocellular carcinoma2011
Author(s)
OkabeH, Beppu T, Ishiko T, Chikamoto A, Masuda T, Otao R, Hasita H, Mima K, Takamori H, Baba H.
Organizer
AACR102nd Annual Meeting 2011
Place of Presentation
Orland convention center, FL, USA.
Year and Date
2011-04-02
Related Report