Amelioration of Acute Tubular Necrosis in Ischemic Acute Renal Failure was Impaired in Mice Lucking Hypoxia Inducible Factor-1α Gene
| Project/Area Number |
23791759
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
YAMAGUCHI Kunihisa 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (90464346)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | HIF-1 / 再生 / IRI / ＩＲＩ |
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| Research Abstract |
As a result of DNA microarrays were performed using kidney sample of the IRI model of HIF-1αkd mice and WILD type mice, and having analyzed exhaustive gene expression analysis, some genes which were thought to be associated with a stress reply and an immune response, cellular tissue repair were found to be present. We paid its attention to "CHAC1" gene of those and pushed forward an analysis. By the in vitro confirmation experiment using HK-2 cells, it could be confirmed that the expression of CHAC1 gene rose in real time PCR conspicuously and was able to confirm similar expression ata protein level in Western blot. Based on these results, the inductivity by HIF-1α of CHAC1 is confirmed using CHIP assay, but is progressing now.
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Report
(3 results)
Research Products
(10 results)
