Innovative regenerative medicine of hearing loss after cochlear ischemia by embryonic stem cells
| Project/Area Number |
23791903
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Ehime University |
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Principal Investigator |
TAKAGI Taro 愛媛大学, 医学部附属病院, 医員 (20601024)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 内耳再生 / ES細胞 / 突発性難聴 / 虚血性内耳障害 / 骨髄幹細胞 / 静脈内投与 / 神経前駆細胞 / 再生療法 |
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| Research Abstract |
We investigated the influence of HSCs on progressive hair cell degeneration after transient cochlear ischemia in gerbils. Transient cochlear ischemia was produced by extracranial occlusion of the bilateral vertebral arteries. HSCs were administrated via femoral vein. Transplanted cells were confirmed in the spiral modiolar artery but was not found in the organ of Corti. Sequential changes in hearing were assessed by ABR. HSCs group was significantly decreased ABR threshold shift compared to the control group. The average ratio of dead/intact cells of inner hair cells was significantly smaller in the HSCs group than control group. The increasing of trophic factors protein level in the organ of Corti in the HSCs group indicated that HSCs upregulated the trophic factors. The intravenous injected HSCs could prevent hair cell degeneration and ameliorate hearing impairment. These results suggested that HSCs had therapeutic potential possibly through paracrine effects.
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Report
(4 results)
Research Products
(7 results)
