Elucidation of the dynamic interaction with the cell - cell adhesion and the cell - extracellular matrix adhesion in the epidermal keratinocytes.

• Project/Area Number: 23792050
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Osaka City University
• Principal Investigator: TATEISHI CHIHARU 大阪市立大学, 医学(系)研究科(研究院), 講師 (40597308)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 細胞―細胞接着 / 細胞―細胞外マトリックス接着 / 細胞―細胞外マトリクス接着 / 細胞-細胞接着細 / 細胞-細胞外マトリクス接着 / live cell imaging / ヘミデスモゾーム / 細胞-細胞接着

Research Abstract

The purpose of our study is to examine the dynamic interaction of cell - cell adhesion and cell - extracellular matrix adhesion in the epidermal keratinocytes. We used live cell imaging method with confocal laser microscope. We made dual transfection alpha - actinin of focal adhesion protein (CFP) plasmid and beta4 integrin of hemidesmosome (YFP) plasmid in normal human epidermal keratinocytes to visualize targets of extracellular matrix adhesion. We investigated methods how to inhibit cell - cell adhesion.

Research Products

• [Journal Article] A rare case of male bullous lupus erythematosus complicated with subsequent annular hypopigmentation (2014)
  • Author(s): Itoi S, Tanemura A, Tsuji C, Kitaba S, Yokomi A, Katayama I, Tateishi C, Tsuruta D
  • Journal Title: Case Rep Dermatol Volume: 6(1) Pages: 91-97
  • Peer Reviewed
• [Journal Article] Successful treatment of betamethasone syrup on autoimmune esophagitis (2014)
  • Author(s): Kohata Y, Fujiwara Y, Kato K, Tanaka F, Yamagami H, Tanigawa T, Shiba M, Watanabe K, Watanabe T, Tominaga K, Tateishi C, Tsuruta D, Arakawa T
  • Journal Title: Am J Gastroenterol Volume: 109(3) Pages: 451-453
  • Peer Reviewed
• [Journal Article] Spatial and temporal control of laminin-332 and -511 expressions during hair morphogenesis (2014)
  • Author(s): Imanishi H, Tsuruta D, Tateishi C, Sugawara K, Kobayashi H, Ishii M, Kishi K
  • Journal Title: Med Mol Morphol. Med Mol Morphol Volume: 47(1) Pages: 38-42
  • Peer Reviewed
• [Journal Article] Three cases of linear IgA/IgG bullous dermatosis showing IgA and IgG reactivity with multiple antigens, particularly laminin-332 (2013)
  • Author(s): Sakaguchi M, Bito T, Oda Y, Kikusawa A, Nishigori C, Munetsugu T, Yokozeki H, Itotani Y, Niguma T, Tsuruta D, Tateishi C, Ishii N, Koga H, Hashimoto T
  • Journal Title: JAMA Dermatol Volume: 149(11) Pages: 1308-1313
  • Peer Reviewed
• [Journal Article] A successful treatment with ustekinumab in a case of antilaminin-γ1 pemphigoid associated with psoriasis (2013)
  • Author(s): Majima Y, Yagi H, Tateishi C, Groth S, Schmidt E, Zillikens D, Koga H, Hashimoto T, Tokura Y
  • Journal Title: Br J Dermatol Volume: 168(6) Pages: 1367-1369
  • Peer Reviewed
• [Journal Article] Two cases of infantile linear immunoglobulin A/immunoglobulin G bullous dermatosis (2012)
  • Author(s): Kanayama Y, Tsuruta D, Tateishi C, Hasegawa Y, Amo K, Fukai K, Kobayashi H, Ishii M
  • Journal Title: J Dermatol Volume: 39(2) Pages: 176-178
  • NAID: 10031184856
  • Peer Reviewed
• [Journal Article] Linear immunoglobulin A/immunoglobulin G bullous dermatosis associated with Vogt-Koyanagi-Harada disease (2011)
  • Author(s): Yanagihara S, Mizuno N, Naruse A, Tateishi C, Tsuruta D, Ishii M
  • Journal Title: J Dermatol Volume: 38(8) Pages: 798-801
  • NAID: 10031147091
  • Peer Reviewed
• [Presentation] 角層下膿疱症(Subcorneal pustular dermatosis/SPD)型IgA天疱瘡の一例 (2013)
  • Author(s): 立石千晴,小川晋司,木村友香,鶴田大輔
  • Organizer: 第64回日本皮膚科学会中部支部学術大会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 抗ラミニンγ1(p200)類天疱瘡の1例 (2013)
  • Author(s): 三和拓人,西村景子,有馬豪,矢上晶子,黒田誠,古賀浩嗣,立石千晴,橋本隆,松永佳世子
  • Organizer: 第112回日本皮膚科学会総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 尋常性乾癬に合併したBP180C末端抗体を有する抗ラミニンγ1類天疱瘡の1例 (2013)
  • Author(s): 橋爪志保,安齋眞一,山本三幸,松岡保子,武市幸子,小坂素子,川名誠司,立石千晴,古賀浩嗣,橋本隆
  • Organizer: 第76回東京支部学術大会
  • Place of Presentation: 京王プラザホテル
• [Presentation] 7型コラーゲンとラミニン332を含む多様な自己抗体を形成した線状IgA/IgG水疱性皮膚症の1例 (2011)
  • Author(s): 織田好子,末廣尚美,菊澤亜夕子,坂口正展,尾藤利憲,立石千晴,古賀浩嗣,橋本隆,錦織千佳子
  • Organizer: 第111回日本皮膚科学会総会
  • Place of Presentation: 国立京都国際会館