Elucidation of the effect of the volatile anesthetics in the mechanism of the cell death by the myocardial ischemia-reperfusion injury

• Project/Area Number: 23792396
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: INAMURA Yoshitaka 大阪歯科大学, 歯学部, 講師(非常勤) (80595794)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 虚血性心疾患 / 心筋保護 / 揮発性麻酔薬 / オートファジー / アポトーシス

Research Abstract

Sevoflurane postconditioning reduced the infarct size by induction of autophagy. expression of LC3-II and the ratio of LC3-II/I were significantly increased in sevoflurane groups. Administration of 3-MA abolished these cardioprotective effects of sevofluranegroups. Expression of LC3-II and the ratio of LC3-II/I were significantly increased in POST and POST-L. Administration of 3-MA abolished these cardioprotective effects of POST. Sevoflurane postconditioning induces autophagy which plays an important role in cardioprotection against IR.

Research Products

• [Journal Article] Sevoflurane confers additive cardioprotection to ethanol preconditioning associated with enhanced phosphorylation of glycogen synthase kinase-3s and inhibition of mitochondrial permeability transitionpore opening (2012)
  • Author(s): Onishi A, Miyamae M, Inoue H, Kaneda K, Okusa C, Inamura Y, Shiomi M, Koshinuma S, Momota Y, Figueredo VM
  • Journal Title: J Cardiothorac Vasc Anesth Volume: - Issue: 5 Pages: 916-924
  • DOI: 10.1053/j.jvca.2012.10.002
  • Peer Reviewed
• [Journal Article] Sevoflurane Confers Additive Cardioprotection to Ethanol Preconditioning Associated With Enhanced Phosphorylation of Glycogen Synthase Kinase-3β and Inhibition of Mitochondrial Permeability Transition Pore Opening. (2012)
  • Author(s): Onishi A.
  • Journal Title: J Cardiothorac Vasc Anesth. Volume: -
  • Peer Reviewed
• [Presentation] 心筋虚血再灌流障害におけるnecroptosisの関与 (2012)
  • Author(s): 越沼静
  • Organizer: 第40回日本歯科麻酔学会総会・学術集会
  • Place of Presentation: アクロス福岡(福岡市)
  • Year and Date: 2012-10-06
• [Presentation] セボフルランポストコンディショニングの虚血心筋保護効果にはオートファジーの誘導が必要である (2012)
  • Author(s): 稲村吉高
  • Organizer: 第40回日本歯科麻酔学会総会・学術集会
  • Place of Presentation: アクロス福岡(福岡市)
  • Year and Date: 2012-10-06
• [Presentation] セボフルラン・エタノールによる心筋プレコンディショイングの細胞内メカニズムの解明 (2012)
  • Author(s): 大西杏奈
  • Organizer: 第9回日本歯科麻酔学会中国・四国地方会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2012-07-29
• [Presentation] セボフルランポストコンディショニングの虚血心筋保護効果にはオートファジーの誘導が必要である (2012)
  • Author(s): 稲村吉高
  • Organizer: 日本麻酔科学会第59回学術集会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2012-06-08
• [Presentation] 心筋虚血前のオートファジーの誘導は長時間虚血により消失したセボフルランプレコンディショニング効果を回復させる (2012)
  • Author(s): 塩見真由美
  • Organizer: 日本麻酔科学会第59回学術集会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2012-06-08
• [Presentation] Sevoflurane postconditioning reduces myocardial ischemia-reperfusion injury by induction of autophagy in isolated guinea pig hearts (2011)
  • Author(s): Inamura Y
  • Organizer: 9th International Congress on Coronary Artery Disease (ICCAD) from Prevention to Intervention 2011
  • Place of Presentation: Venive (Italy)
  • Year and Date: 2011-10-25
• [Presentation] Dipyridamole-induced cardiac preconditioning persists for3 days after discontinuing therapy and is mediated through Akt activation (2011)
  • Author(s): Okusa C
  • Organizer: 9th International Congress on Coronary Artery Disease (ICCAD) from Prevention to Intervention 2011
  • Place of Presentation: Venive (Italy)
  • Year and Date: 2011-10-25
• [Presentation] Dipyridamole-induced cardiac preconditioning persists for 3 days after discontinuing therapy and is mediated through Akt activation. (2011)
  • Author(s): Okusa C.
  • Organizer: 9th International Congress on Coronary Artery Disease (ICCAD) from Prevention to Intervention 2011
  • Place of Presentation: Venice (Italy)
• [Presentation] Sevoflurane postconditioning reduces myocardial ischemia-reperfusion injury by induction of autophagy in isolated guinea pig hearts. (2011)
  • Author(s): Inamura Y.
  • Organizer: 9th International Congress on Coronary Artery Disease (ICCAD) from Prevention to Intervention 2011
  • Place of Presentation: Venice (Italy)
• [Presentation] 心筋虚血前のオートファジーの誘導は長時間虚血により消失したセボフルランプレコンディショニング効果を回復させる．
  • Author(s): 塩見真由美
  • Organizer: 日本麻酔科学会第59回学術集会
  • Place of Presentation: 神戸国際会議場（神戸市）
• [Presentation] セボフルランポストコンディショニングの虚血心筋保護効果にはオートファジーの誘導が必要である．
  • Author(s): 稲村吉高
  • Organizer: 日本麻酔科学会第59回学術集会
  • Place of Presentation: 神戸国際会議場（神戸市）
• [Presentation] セボフルラン・エタノールによる心筋プレコンディショイングの細胞内メカニズムの解明．
  • Author(s): 大西杏奈
  • Organizer: 第9回日本歯科麻酔学会中国・四国地方会
  • Place of Presentation: 岡山大学（岡山市）
• [Presentation] セボフルランポストコンディショニングの虚血心筋保護効果にはオートファジーの誘導が必要である．
  • Author(s): 稲村吉高
  • Organizer: 第40回日本歯科麻酔学会総会・学術集会
  • Place of Presentation: アクロス福岡（福岡市）
• [Presentation] 心筋虚血再灌流障害におけるnecroptosisの関与．
  • Author(s): 越沼 静
  • Organizer: 第40回日本歯科麻酔学会総会・学術集会
  • Place of Presentation: アクロス福岡（福岡市）