New practical technique for Protein Crystallization with Mebiol Gel

• Project/Area Number: 23860028
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied materials science/Crystal engineering
• Research Institution: Osaka University
• Principal Investigator: SUGIYAMA Shigeru 大阪大学, 理学(系)研究科(研究院), 特任准教授 (90615428)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: メビオールゲル / アガロースゲル / 固相ゲル中結晶化 / タンパク質結晶化 / X線構造解析 / 創薬 / 分子設計 / 固相ゲル中結晶化法 / Ｘ線構造解析 / 相転移

Research Abstract

In this study, we successfully crystallized three proteins in Mebiol gel. Our analysis indicates that Mebiol gel provides new possibilities for overcoming the inherent problem of high-temperature damage to the proteins. We also investigated the nucleation rate of protein crystals in the presence of Mebiol gel and found that Mebiol gel increased the number of protein crystals. Furthermore, the Mebiol gel surrounding the crystals reduces the influence of osmotic shock in high-ionic-strength solutions. This technique will open up a new dimension for the crystallization of biological macromolecules in hydrogels.

Research Products

• [Journal Article] A novel approach for protein crystallization by a synthetic hydrogel with thermoreversible gelation polymer (2013)
  • Author(s): S. Sugiyama, N. Shimizu, G. Sazaki, M. Hirose, Y. Takahashi, M. Maruyama, H. Matsumura, H. Adachi, K. Takano, S. Murakami, T. Inoue, and Y. Mori
  • Journal Title: Cryst. Growth Des. Volume: 13 Pages: 1899-1904
  • Peer Reviewed
• [Journal Article] Effects of Gel-Solution Interfaces on Femtosecond Laser-Induced Nucleation of Protein (2013)
  • Author(s): S. Nakayama, H. Yoshikawa, R. Murai, M. Kurata, M. Maruyama, S. Sugiyama, Y. Aoki, Y. Takahashi, M. Yoshimura, S. Nakabayashi, H. Adachi, H. Matsumura, T. Inoue, K. Takano, S. Murakami, Y. Mori
  • Journal Title: Cryst. Growth Des. Volume: 13 Pages: 1491-1496
  • Peer Reviewed
• [Journal Article] 固相ハイドロゲル中でのタンパク質結晶化 (2012)
  • Author(s): 杉山成、松村浩由、丸山美帆、佐崎元、廣瀬未果、安達宏昭、高野和文、村上聡、井上豪、森勇介
  • Journal Title: 日本結晶学会誌 Volume: 54巻 Pages: 300-303
  • NAID: 10031129966
  • Peer Reviewed
• [Journal Article] Growth of protein crystals in hydrogels prevents osmotic shocks (2012)
  • Author(s): S. Sugiyama, M. Maruyama, G. Sazaki, M. Hirose, H. Adachi, K. Takano, S. Murakami, T. Inoue, Y. Mori, and H. Matsumura
  • Journal Title: J. Am. Chem. Soc. Volume: 134 (13) Pages: 5786-5789
• [Journal Article] Three-dimensional, non- invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography (2012)
  • Author(s): N. Nishizawa, S. Ishida, M. Hirose, S.Sugiyama, T. Inoue, Y. Mori, K. Itoh, and H. Matsumura
  • Journal Title: Biomedical Optics Express Volume: 3 Pages: 735-740
• [Journal Article] Growth of protein crystals in hydrogels prevents osmotic shocks (2012)
  • Author(s): Shigeru Sugiyama
  • Journal Title: J. Am. Chem. Soc. Volume: 134 Pages: 5786-5789
  • Peer Reviewed
• [Journal Article] 固相ハイドロゲル中でのタンパク質結晶化 (2012)
  • Author(s): 杉山成
  • Journal Title: 日本結晶学会誌 Volume: 54 Pages: 300-303
  • NAID: 10031129966
  • Peer Reviewed
• [Journal Article] Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography (2012)
  • Author(s): Norihiko Nishizawa
  • Journal Title: Biomedical Optics Express Volume: 3 Pages: 735-740
  • Peer Reviewed
• [Presentation] 創薬スクリーニングを容易にするタンパク質結晶の機械的強化技術 (2013)
  • Author(s): 杉山成、松村浩由、丸山美帆子、佐崎元、廣瀬未果、安達宏昭、高野和文、村上聡、井上豪、森勇介
  • Organizer: 第24回高分子ゲル研究討論会
  • Place of Presentation: 東京大学 山上会館
  • Year and Date: 2013-01-16
• [Presentation] 蛋白質の固相ゲル結晶育成技術の開発とその機能解析 (2012)
  • Author(s): 杉山成、安達宏昭、高野和文、村上聡、井上豪、森勇介、松村浩由
  • Organizer: 第42回結晶成長国内会議
  • Place of Presentation: 九州大学筑紫キャンパス
  • Year and Date: 2012-11-09
• [Presentation] 超高分解能OCTによるタンパク質結晶の3次元・高機能イメージング (2012)
  • Author(s): 石田周太郎、西澤典彦、廣瀬未果、杉山成、井上豪、森勇介、伊東一良、松村浩由
  • Organizer: 日本光学会年次学術講演会
  • Place of Presentation: 東京 タワーホール船堀
  • Year and Date: 2012-10-23
• [Presentation] Hydrogel Protects Protein Crystals From Osmotic Shock (2012)
  • Author(s): S. Sugiyama, M. Maruyama, G. Sazaki, M. Hirose, H. Adachi, K. Takano, S. Murakami, T. Inoue, Y. Mori, and H. Matsumura
  • Organizer: 14th International Conference on the Crystallization of Biological Macromolecules (ICCBM14)
  • Place of Presentation: Huntsville, Arabama USA
  • Year and Date: 2012-09-27
• [Presentation] Effect of solution stirring on protein crystal growth on high concentrated hydrogel (2012)
  • Author(s): Y. Aoki, M. Maruyama, Y. Takahashi, M. Yo sh imu r a, H. Yo sh ik awa, S. Sug iyama, H. Adachi, K. Takano, S. Murakami, H. Matsumura, T. Inoue and Y. Mori
  • Organizer: 14th International Conference on the Crystallization of Biological Macromolecules (ICCBM14)
  • Place of Presentation: Huntsville, Arabama USA
  • Year and Date: 2012-09-27
• [Presentation] 超高分解能OCTによるタンパク質結晶の3次元・非侵襲断層イメージング (2012)
  • Author(s): 石田周太郎、西澤典彦、廣瀬未果、杉山成、井上豪、森勇介、伊東一良、松村浩由
  • Organizer: 第73回応用物理学会学術講演会
  • Place of Presentation: 愛媛 松山大学文京キャンパス
  • Year and Date: 2012-09-12
• [Presentation] Growth of Protein Crystals in Semi-Solid Hydrogel (2012)
  • Author(s): S. Sugiyama
  • Organizer: Institute for Protein Research Seminar
  • Place of Presentation: Osaka University
  • Year and Date: 2012-06-18
• [Presentation] Growth of Protein Crystals in Semi-Solid Hydrogel (2012)
  • Author(s): Shigeru Sugiyama
  • Organizer: Institute for Protein Research Seminar
  • Place of Presentation: 大阪大学蛋白質研究所
  • Invited
• [Presentation] Hydrogel Protects Protein Crystals From Osmotic Shock (2012)
  • Author(s): Shigeru Sugiyama
  • Organizer: 14th International Conference on the Crystallization of Biological Macromolecules (ICCBM14)
  • Place of Presentation: 米国アラバマ州ハンツビル
• [Presentation] Effect of solution stirring on protein crystal growth on high concentrated hydrogel (2012)
  • Author(s): Yusuke Aoki
  • Organizer: 14th International Conference on the Crystallization of Biological Macromolecules (ICCBM14)
  • Place of Presentation: 米国アラバマ州ハンツビル
• [Presentation] 薬剤スクリーニングを容易にするタンパク質結晶の機械的強化技術 (2012)
  • Author(s): 杉山成
  • Organizer: 第12回日本蛋白質科学会年会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] Towards an understanding of lipid-protein interactions, new approaches for producing high-quality protein crystals (2011)
  • Author(s): S. Sugiyama
  • Organizer: International ERATO Symposium on Lipid Structures in and around Proteins
  • Place of Presentation: Hotel Hankyu Expo Park
  • Year and Date: 2011-11-14
• [Presentation] Towards an understanding of lipid-protein interactions, new approaches for producing high-quality protein crystals (2011)
  • Author(s): 杉山成
  • Organizer: International ERATO Symposium on Lipid Structures in and around Proteins
  • Place of Presentation: ホテル阪急エキスポパーク(大阪府)
  • Year and Date: 2011-11-14
• [Remarks] 平成24年度 日本結晶成長学会賞受賞者紹介
  • URL: http://www.jacg.jp/jacg/japanese/frame_main/16/2012_JACGAWARD.html
• [Remarks] 固相ハイドロゲル中でのタンパク質結晶化
  • URL: https://www.jstage.jst.go.jp/article/jcrsj/54/5/54_300/_pdf
• [Remarks] ２０１２年度 プレスリリース
  • URL: http://www.sci.osaka-u.ac.jp/press_release/index-jp.html