| Budget Amount *help |
¥403,650,000 (Direct Cost: ¥310,500,000、Indirect Cost: ¥93,150,000)
Fiscal Year 2016: ¥65,000,000 (Direct Cost: ¥50,000,000、Indirect Cost: ¥15,000,000)
Fiscal Year 2015: ¥89,700,000 (Direct Cost: ¥69,000,000、Indirect Cost: ¥20,700,000)
Fiscal Year 2014: ¥94,250,000 (Direct Cost: ¥72,500,000、Indirect Cost: ¥21,750,000)
Fiscal Year 2013: ¥86,450,000 (Direct Cost: ¥66,500,000、Indirect Cost: ¥19,950,000)
Fiscal Year 2012: ¥68,250,000 (Direct Cost: ¥52,500,000、Indirect Cost: ¥15,750,000)
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| Outline of Final Research Achievements |
Circadian clock fit metabolisms of living cells with the day-night cycle of the Earth. The basic mechanism of the circadian clock has been assumed to the autoregulation of clock gene expression in the cell. However, three Kai proteins of cyanobacteria generate precise and stable 24 h rhythm by mixing three proteins with ATP in the test tube. In this project, we focused on biochemical and structural analyses of KaiC and its clock mutants to elucidate the timing mechanism installed in the clock protein KaiC. We found that KaiC ticks time by coupling harmonic pacemaker with driver oscillator by the escapement mechanism, which can be found in the mechanical pendulum clock or classical watches. We are proposing the new clock model for circadian clock and surveying similar mechanisms in eukaryotic clock system.
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