Budget Amount *help |
¥198,380,000 (Direct Cost: ¥152,600,000、Indirect Cost: ¥45,780,000)
Fiscal Year 2016: ¥27,820,000 (Direct Cost: ¥21,400,000、Indirect Cost: ¥6,420,000)
Fiscal Year 2015: ¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
Fiscal Year 2014: ¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
Fiscal Year 2013: ¥47,710,000 (Direct Cost: ¥36,700,000、Indirect Cost: ¥11,010,000)
Fiscal Year 2012: ¥66,690,000 (Direct Cost: ¥51,300,000、Indirect Cost: ¥15,390,000)
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Outline of Final Research Achievements |
By using physiological molecular information, environmental conditions can be adjusted optimally to grow crops in plant growth factories. Such a control method uses physiological information to optimize energy efficiency and product quality control in plant growth factories, and is known as the “speaking cell approach” (SCA). In the present study, methods for on-site/real time cellular and molecular measurement techniques have been developed by using a cell pressure probe and probe electrospray ionization mass spectrometry for SCA. By mixing ionic liquid with the silicone oil in the pressure probe, pico pressure probe electrospray ionization mass spectrometry was developed to measure metabolite concentrations of single cells of plants. Hence, the water status and metabolite concentrations of single cells of plants can be measured simultaneously to evaluate the physiological conditions in real time to control environmental conditions in greenhouses.
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